Objective To investigate the application value of 16S rDNA-based detection technique in the rapid screening for and confirmation of gonococcemia. Methods A 41-year-old male patient was hospitalized for recurrent regular fever and chills for 1 month. Several days before the admission, he developed urgent micturition, frequent micturition and pain in urination, anemia with emaciation appearance, slightly pale-looking skin and mucous membranes. No petechia, skin eruption or superficial lymphadenectasis was observed, but routine blood test and urine test results were abnormal. No abnormality was found in stool test or hepatic and renal function. DNA was extracted from the blood of the patient and subjected to PCR for the amplification of 16S rDNA followed by sequence analysis and homology analysis. At the same time, bacterial culture of blood and drug sensitivity test for the bacterial isolate were performed. Results Homology analysis indicated that the amplicon sequence was consistent with the known sequence of 16S rDNA of Neisseria gonorrhoeae in GeneBank, which agreed well with the culture result of peripheral blood. Conclusion The detection of 16s rDNA with PCR from peripheral blood is highly efficient, specific, sensitive, rapid and accurate for the screening for and confirmation of gonococcemia.

Objective To study the relationship of T, B and NK lymphocytes with the pathogenesis of chronic urticaria. Methods Flow cytometry was applied to assess the proportion of T, B and NK lymphocyte subgroups in the peripheral blood of 51 patients with chronic urticaria and 30 sex and age-matched human controls. The CD4:CD8 ratio was calculated. Moreover, the symptoms, disease course and response to antihistamines of these patients were evaluated by one physician. Results The percentage of CD8+ T and NK cells, CD4:CD8 ratio were (27.20±8.22)%, (21.20±10.84)% and 1.48±0.62, respectively, in these patients,(29.9±3.74)%, (17.5±3.56)%, 1.24±0.27, respectively, in the controls; the differences were significant between the two groups (all P＜0.05). Decreased levels of CD3+ T cells, CD8+ T cells and B cells were noted in patients resistant to antihistamines compared with those responsive to antihistamines[(61.81±11.70)% vs (75.74±2.36)%, (24.00±7.79)% vs (34.22±9.30)%, (10.78±2.07)% vs (15.25±4.10)%, P＜0.05, 0.01, 0.05, respectively)], while the CD4:CD8 ratio and percentage of NK cells were increased in antihistamine-resistant patients compared to those in antihistamine-sensitive patients [1.67±0.76 vs 1.17±0.41, (28.61±12.62)% vs (12.78±6.02)%, both P＜0.01 ]. In these patients with chronic urticaria, the percentages of CD3+ T and CD8+ T cells were negatively correlated with the symptom scores (R = -0.31, -0.28, respectively, both P＜0.05 ), while the percentage of B cells was positively correlated with the symptom scores and disease course (R = 0.53, 0.55, respectively, both P＜0.01 ). Conclusions There is an abnormality in the proportion of T, B and NK lymphocyte subgroups in patients with chronic urticaria,which indicates that humoral immunity may be involved in the pathogenesis of chronic urticaria and the mechanism for responsiveness to antihistamine.

Objective To characterize the prevalence and occurrence of respiratory viruses in chil-dren in Southern China. Methods Respiratory virus were identified from nasopharyngeal aspirates and throat swabs collected from children with bronchiolitis, bronchopneumonia and asthmatic bronchitis who visi-ted the Pediatrics of Affiliated Hospital to Shantou University, Shenzhen Fourth People's Hospital, Jieyang People's Hospital, during the period of June 2006 to June 2008. Respiratory virus was detected by multiplex PCR. Results Viruses were detected in 362 patients(52.77% ), among them, RSV infection was the most frequent, 31.22% of 113 patients. RHV was found in 16.85% (61 patients), IVA in 14.36% (52 pa-tients), ADV in 9.67% (35 patients), PIV in 16. 02% (58 patients), hBOV in 6. 08% (22 patients), hMPV in 4.97% ( 18 patients) and IVB in 0. 83% (3 patients). Conclusion The data indicate that RSV, RHV and IVA is an important etiological agent for respiratory infections in children during the survey period. RSV, IVA combined other virus are the most virus for combined infection, and the manages are worked out by the doctor for the diagnosis and treatment depended on the detected results of the pathogen.

Objective To investigate the drug-resistance of Staphylococcus aureus induced by erythromycin in vitro and its changes in growth and susceptibility of antibiotics.Methods Erythromycin in vitro induction was conducted with the S.au-reus reference strain ATCC25923 on the serial of erythromycin agar plates.The growth of S.aureus,and the susceptibility a-gainst other antibiotics was compared after induced to the parent strain.Results Resistance to erythromycin was successful-ly,and themaximum MIC was over 256 mg/L.The Erythromycin-resistant S.aureus grew much slower than the susceptible parent,and the strains didn’t have cross-resistance to other antibiotics.Conclusion S.aureus could be induced resistance in vitro by erythromycin,and this resistance inherited stably.Some phenotype and biochemical characteristic features of the strain were changed after induced.

Objective:To explore the therapeutic effect of peroral endoscopic myotomy (POEM) for primary achalasia (AC) in patients aged over 60 years.Methods:Data of 146 patients aged ≥60 years (the elderly group) and 146 patients aged 18-59 years (the adult group) who received POEM from November 2010 to September 2019 at the Digestive Endoscopy Center of PLA General Hospital were retrospectively analyzed. Baseline data, surgery data, surgery-related complications and surgery-related efficacy were compared.Results:There was no significant difference in gender, Ling classification, HRM classification or previous treatment between the two groups ( P>0.05). All 292 patients successfully underwent POEM surgery. The clinical success (Eckardt score ≤3) rates in the elderly group and the adult group were 96.33% (105/109) and 96.77% (90/93), respectively with no significant difference between the two groups ( χ2=0.030， P>0.05). There was no significant difference in the length of myotomy between the two groups (7.09±2.49 cm VS 7.12±2.24 cm， t=0.472， P>0.05). Complications occurred in 26 cases (17.81%) in the elderly group and 21 cases (14.38%) in the adult group with no significant difference between the two groups ( χ2=0.634， P>0.05). There was no significant difference in the postoperative hospital stay (12.61±9.69 days VS 11.00±4.43 days, t=1.825, P>0.05) or the incidence of gastroesophageal reflux [43.33% (13/30) VS 51.52% (17/33), χ2=0.422, P>0.05] between the elderly group and the adult group. Conclusion:The efficacy of POEM for AC patients over 60 years old is equivalent to that of adult patients, and the incidence of complications is similar. POEM is safe and effective for AC patients over 60 years old.

Objective:To investigate the related genes, signaling pathways and possible mechanisms of malignant transformation of human papillomavirus type 16 (HPV-16) immortalized cervical epithelial cell line H8.Methods:The malignant transformed H8 cell model was constructed, and the changes of cell invasion ability and cell migration ability of H8 cells after malignant transformation were detected by Transwell assay, and the changes of clone formation ability of H8 cells after malignant transformation were detected by plate clone formation assay. Total RNA was extracted from malignant transformed H8 cells and H8 cells, and the two groups of cells were sequenced by transcriptome using Illumina novaseq 6000 sequencing platform, differentially expressed genes (DEGs) were identified and analyzed, and Gene Ontology (GO) function enrichment analysis, Kyoto Encyclopedia of genes and genomes (KEGG) pathway enrichment analysis and protein-protein interaction were performed.Results:The invasion ability, migration ability and clone formation ability of malignant transformed H8 cells significantly increased as compared to H8 cells. A total of 203 differentially expressed genes were identified in H8 cells before and after malignant transformation, of which 98 were up-regulated and 105 down-regulated. GO enrichment analysis showed that DEGs were mainly involved in biological processes such as cellular processes, biological regulation, and metabolic processes. KEGG pathway enrichment analysis showed that DEGs were mainly enriched in alanine, aspartate and glutamate metabolic pathway, glycine, serine and threonine metabolism pathway, p53 signaling pathway and TGF-β signaling pathway, PI3K-Akt signaling pathway. PPI analysis screened 10 hub genes including DDIT3, TRIB3 and ASNS.Conclusions:Compared with H8 cells, malignant transformed H8 cells have a large number of differentially expressed genes and pathways at the transcriptional level, which could further provide new ideas for the mechanism of malignant transformation and carcinogenesis as well as finding new targets for the prevention of malignant transformation.

Objective To investigate the expression and prognostic value of serum antimicrobial peptide LL37 and human soluble scavenger receptor cysteine-rich domain-containing protein(SSC5D)in elderly patients with chronic heart failure(CHF).Methods A total of 100 CHF patients admitted to Suzhou Kowloon Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2017 to January 2022 were selected as the CHF group.According to the prognosis,these patients were divided into good group(n=60)and poor prognosis group(n=40).Another 100 healthy examinees were selected as the control group.Enzyme-linked immunosorbent assay was used to detect serum levels of LL37 and SSC5D.The differences in serum LL37 and SSC5D levels between CHF group and control group were compared.Spearman correlation analysis was used to analyze the correlation between serum LL37 and SSC5D.Kaplan-Meier survival curve was used to analyze the relationship between serum LL37 and SSC5D expression and the prognosis of CHF patients.COX regression analysis was used to analyze the factors affecting the prognosis of CHF patients.ROC curve was used to analyze the predictive value of serum LL37 and SSC5D for poor prognosis in elderly CHF patients.Result The levels of serum LL37(771.38±158.25 ng/ml)and SSC5D(15 789.35±1 306.25 pg/ml)in CHF group were higher than those in control group(526.23±115.58 ng/ml,8 938.72±858.29 pg/ml),and the differences were significant(t=12.510,43.830,all P＜0.001).Spearman correlation analysis showed that there was a significant positive correlation between serum LL37 and SSC5D in CHF patients(r=0.629,P＜0.001).The serum LL37 and SSC5D levels in CHF patients were positively correlated with NYHA cardiac function classification(r=0.776,0.751,all P＜0.001).The survival rate of the high-level LL37 group was lower than that of the low-level LL37 group(38.18％vs 86.67％),and the difference was significant(Log rankx2=24.242,P＜0.001).The survival rate of the high level SSC5D group was lower than that of the low level SSC5D group(37.74％vs 85.10％),and the difference was significant(Log rank x2=23.291,P＜0.001).Compared with the good prognosis group,the poor prognosis group had a higher proportion of patients over 70 years old,proportion of patients with NYHA cardiac function class Ⅲ+Ⅳ,and serum LL37 and SSC5D levels,and the differences were significant(x2=10.774,4.118,t=4.723,14.059,all P＜0.05).Multivariate COX analysis showed that age＞70 years(OR=1.515,95％CI:1.224～1.858),NYHA cardiac function class Ⅲ+Ⅳ(OR=1.236,95％CI:1.198～1.963),high level of LL37(OR=1.705,95％CI:1.163～2.582)and high level of SSC5D(OR=1.591,95％C1:1.052～1.916)were independent risk factors for the prognosis of CHF patients.ROC curve analysis showed that the area under the curve of serum LL37 combined with SSC5D for predicting poor prognosis of CHF in the elderly was larger than that of serum LL37 and SSC5D alone,and the differences were significant(Z=2.834,2.168,P=0.005,0.030).Conclusion The serum LL37 and SSC5D levels are increased in patients with CHF,and both are risk factors for poor prognosis in patients with CHF,which can be used as clinical indicators to evaluate the prognosis of CHF.