Objective:To establish an HPLC method for the determination gensenoside Rg1 , Rb1 and notogensenoside R1 in Sanqi Jiegu pills and to investigate the stability of saponins in the granulation process. Methods:The separation was performes on a COSMO-SIL 5 C18 MS-II(250 mm × 4. 6 mm,5μm)column with gradient elution using water and acetonitrile as the mobile phase, the flow rate was 1. 0 ml·min-1 , the detection wavelength was 203nm, the column temperature was 30℃, and the injection volume was 10 μl. The content change of triterpene saponins in Sanqi Jiegu pills prepared by the granulation process at different drying temperature was al-so investigated. Results:The linearity of notogenoside R1 , gensenoside Rg1 and gensenoside Rb1 was good within the range of 0. 211 8-2.648 0 μg·ml-1(r=0.999 7),0.566 1-7.076 0 μg·ml-1(r=0.999 7) and 0.317 2-3.964 5μg·ml-1(r=0.999 7) with the average recovery of 98. 2%(RSD=0. 82%,n=6), 98. 1%(RSD=0. 72%,n=6) and 98. 1%(RSD=0. 59%,n=6),respectively. The content of saponins in Saqi Jiegu pills was decreased obviously with the increase of the drying temperature. Conclusion:The meth-od is simple, quick and reliable, and can be applied in the quality control of Sanqi Jiegu pills. It is recommended that the drying tem-perature in the granulation process should be controlled below 60℃.

OBJECTIVE:To study the improvement effect of Celosia cristata n-butanol extracts on dysfunctional uterine bleed-ing of rats,and explore its mechanism. METHODS:60 pregnant SD rats were randomly divided into blank group,model group, Gongxuening capsule group (positive control,0.07 g/kg) and C. cristata n-butanol extracts high-dose,medium-dose,low-dose groups(4.32,2.16,1.08 g/kg),10 in each group. Except for the blank group,rats in other groups were intragastrically given mife-pristone and misoprostol on 7th of pregnancy for resulting incomplete abortion to induce models of dysfunctional uterine bleeding. Then rats in administration groups were intragastrically given relevant medicines,rats in blank group and model group were intra-gastrically given normal saline once every morning and evening,for 7 d. On 8th d of pregnancy,uterine bleeding amount,and thromboxane (TXA2),prostacyclin (PGI2) and tumor necrosis factor α(TNF-α) contents in serum were determined. RESULTS:Compared with blank group,uterine bleeding amount in model group was significantly increased(P<0.01),TXA2 content in se-rum was significantly reduced,PGI2 and TNF-α contents were significantly increased(P<0.01). Compared with model group,uter-ine bleeding amounts in administration groups were significantly reduced,TXA2 content in serum was significantly increased(P<0.01);PGI2 and TNF-α contents in serum in Gongxuening capsule group and C. cristata n-butanol extracts high-dose group and TNF-α content in serum in C. cristata n-butanol extracts medium-dose group were significantly reduced (P<0.01). CONCLU-SIONS:C. cristata n-butanol extracts show obvious improvement effect on incomplete drug abortion-induced dysfunctional uterine bleeding of rats,and the mechanism may be related to the regulation of TXA2/PGI2 dynamic balance and inhibition of TNF-α tran-sient secretion.