Two new single chain ribosome—inactivating proteins,polygonin2 from the roots ofPolygonatum odoratum and momordin2,from the seeds of Momordica charantia,inhinited pro-teib synthesis potentially in rabbit reticulocyte lysate,but were relatively low toxic to Molt—4 cells.polygonin2 and momordin2 were conjugated to H65 monoclonal antibody that recognizedhuman T lymphocyte CD5 surface antigen using a heterobifunctional crosslinking reagent 2—iminothiolane.The resulting immunotoxins,referred to as H65—PGN2 and H65—MOR2,showed potent specific cytotoxicity to target cell Molt—4 and human peripheral blood T lympho-cyte,but had no effect on human hematopoietic cells.Injection of Molt—4 cells s.c.into Balb/cnu/nu mice developed into a solid tumor.Administration of the H65—PGN2 or H65—MOR2immunotoxins i.p.10 ug per mice at 48—h intervals suppressed tumor growth specifically.Theresulting showed that clinical studied were warranted to use these conjugates for the therapy ofautoimmune diseases as well as the ex vivo purging of T lymphocyte in ABMT.

Objective:Offer practical experience and theoretical basis for the popularization of Country Feasible Medical Technology(CFMT)in our nation,and explore the best mold and mechanism for popularization of CFMT at the actual situation of our nation,especially to the western rural areas.Methods:We launch the popularization and demonstration of CFMT in two countries of Chongqing City.Results:In 2005 and 2006,we adopted different modes of popularization about CFMT,and obtained different effects;besides,the CFMT reduces the difficult and expensive condition of seeing a doctor to some extent,and wins wide welcome and praise from health workers and masses.Conclusions:For the popularization of CFMT in western rural areas,the best mold is 'government-control & market-guidance' Mold.And the most suitable mechanism is a synergistic effect of many mechanisms,in which Consultation & Combined Action mechanism,Social Mobilization mechanism,Proper Financial Compensation mechanism,and Restriction & Inspiritment mechanism is the four main mechanisms.Besides,to summarize experiences the participation of college and scientific research institute is needed in the whole process of research project.

Objective To explore the promotion model with the sustainable development of appropriate technologies in rural health. Methods After a large-scale government-led, administrative intervention mode (Ⅰ mode) , the mode (Ⅱ mode) dominated by the third sector and participated in by the whole society was used in some counties, and Ⅲ model, an enterprise-led and market economy controlled mode was adopted in Tongliang County. Results The primary care doctor and patient satisfaction in the third sector participation mode, and the personal experiences of doctors in enterprise-led mode were evaluated as excellence. The differences of experience of both doctors and patients between the third sector-led and government-led were statistically significant (P<0.01) ; he third sector-led mode was better than government-led mode. Conclusion It needs to establish a long-term pattern with the government supervision and policy support, the third sector and business management, full community participation, and regulation of market economy rules for the sustainable promotion model of appropriate technologies in rural health.

We built rats model of endometriosis applying autologus endometrial transplants to compare the graft vol-ume of the modeling rats before and after dosing,serum tumor necrosis factor alpha(TNF-α)levels in rats,and the expressions of vascular endothelial cell growth factor(VEGF)in ectopic endometrium.To compare with the control group,the graft volume of DingKunDan group was reduced and the serum TNF-αlevel declined ,and also the ex-pression of VEGF in ectopic endometrium was reduced.Their differences were statistically significant (P <0.05). DingKunDan can inhibit the growth of ectopic endometrium in endometrosis model rats.The mechanism may be re-lated to the decrease of the serum TNF-αlevel and the VEGF expression.

Objective To investigate the electrophysiological characteristics of the atrium in patients with atrial fibrillation (AF) during electrophysiological study (EPS) Methods Seventy two patients with presence of AF during EPS were selected as Group AF , and the other 78 patients without AF as Group control All the patients had no AF history and structural heart diseases P A, P CSd, S1 A1, S2 A2, Max CD, CD zone and ERP HRA were measured as the electrophysiological parameters Results P A, P CSd, S1 A1, S2 A2, Max CD and CD zone were significantly longer in Group AF than in Group control , ERP HRA was shorter in Group AF Conclusion The decreased atrial conduction and the shorter atrial effective refractory period may be the electrophysiological basis for the occurrence of AF

Objective To explore operating efficiency of county-level public hospital by using data envelopment analysis ,provide reference ,and basis for health decision-making .Methods The health resources data of 16 county-level public hospitals were ran-domly selected from 2008 to 2011 ,its relative efficiency were studied by data envelopment analysis method of CCR-CRS and BCC-VRS model ,the change trend from 2008 to 2011 were analyzed through the Malmquist productivity index .Results The average ef-ficiency value was 0 .938 in 16 county-level hospitals in 2011 ,7 hospitals was DEA efficient and 9 hospitals was non-DEA efficient (8 hospitals with Scale efficiency decline gradually ,another one with increasing gradually ) .The total factor productivity increased 2 .5% from 2008 to 2011 average annual ,the productivity first decreased from 2008 to 2009 ,and then increased from 2009 to 2011 . Conclusion The overall efficiency 16 county-level hospitals is not high ,it is necessary to develope hospital scale reasonable , strength hospitals′management level and improve technical ability of doctors .

Objective To find out a new teaching pattern of Medical Statistics for medical postgraduate students.Methods To manifest the longitudinal thought of the medical research process from the teaching idea,the teaching content arrangement and the teaching method utilization.Results To discuss the superiority of the new pattern which provides the content frame and the teaching method based on the integrating theory and the practice.Conclusion The teaching reformation of Medical Statistics must be advantageous in improving the learner’s power of the medical scientific research,including selection of the scientific research topic,application of project,the quality control of the project,analysis of the material and inference of the conclusion,paper writing,self-appraisement and achievement evaluation.

Objective To combine grey relation analysis and partial least squares regression model to establish the forecasting model of per-patient hospitalization expenses .Methods Gray relational analysis was used to filter out the main factors affecting per-patient hospitalization expenses ,and then collinearity was examined between these factors .Partial least squares regression was used to establish prediction model of per-patient hospitalization expenses ,and the prediction accuracy was proved .Results After filtered by gray relational analysis ,the order of the importance of factors affecting per-patient hospitalization expenses was the west-ern medicine fee ,traditional Chinese medicine fees ,diagnosis and treat fees ,other fees ,inspection fees ,bed fees and operation fees . The established partial least squares regression model had a higher accuracy on fitting and prediction ,with low average relative er-ror ,respectively ,-0 .000 2% and 0 .349 3% .Conclusion The gray relational analysis and partial least squares regression are suit-able for the influencing factors and prediction analysis of hospitalization costs .It provides a reference for data with the small sample size and high collinearity between the variables .

Objective To explore the neurasthenia situation of the population attending the postgraduate exam in medical class and its influencing factors .Methods The random cluster sampling method was adopted .The influencing factors were per‐formed the χ2 test and Logistic regression analysis .Results According to the inclusion criteria and exclusion criteria ,36 positive ca‐ses were screened out ,accounting for 8 .89% of participants ,which was higher than the value of the general population ;the statisti‐cally significant differences were noted in the factors of the confidence of attending the postgraduate exam ,stress ,adjusting ability , interpersonal situation ,reading duration ,sleep ,nutrition and dietary (P<0 .05) .Conclusion Attending the postgraduate exam such highly intensitive mental activity is the important reason causing neurasthenia among the population attending the postgraduate ex‐am ,which mainly display in the aspects of stress size ,bearing ability facing stress and regulation .

Objective To understand the health level of people living in county and the situation between the requirement and utilization of health service for rural residents in Chongqing as well as the main influential factors by investigating the basic condition and hospitalization expenditure. Methods Three county hospitals were included in our study: county general hospital, Chinese traditional medicine hospital, and maternal care hospital. The hospitalized patients from the three hospitals were investigated on a certain day by questionaire and the data of discharged patients in a month were collected and analyzed by SAS8.0 software. Results Factors such as the hospital type, sex, age, the cost for sickbed, diagnosis and treatment fee and so on were significantly associated with the total cost for hospitalization. Expensive hospitalization fee harms the quantity and quality of health service required by rural residents. Conclusion The utilization rate of health service by female was lower than by male in rural area. The total utilization rate of health service has been improved since the new policy about rural health service was carried out. People over 45 years old are the main consumers of health service. Peasants are still main consumers to medical organization at county level. Suitable technic of health service for county hospitals should be carried out in time.