Objective To investigate the relationship between expression of CDK1,TFF2 and HPV16/18 infection in uterine cervical carcinomas.Methods Formalin-fixed,paraffin-embedded samples including 50 cases of squamous cell carcinomas,20 cases of cervical intraepithelial neoplasia CINⅡ-Ⅲand 20 cases of CINI and 20 cases of normal cervical tissues were studied.Using Polymer DetectionSystem For Immuno-Histological Staining(PV-9000)as immunohisto-chemical detecting method.the expression of TFF2 and CDK1 in these samples were examined.The infections of HPV type 16,18 DNA were determined by PCR.Results There were significant differences for CDK1 between carcinomas,CIN and normal cervices,respectively(all P＜0.05).Expression of CDKl were correlated with tumor grades(P＜0.05).The positive expression of TFF2 in carcinomas was significantly lower than that in normal cervical tissue and CIN.There was significant differences amongthem(P＜0.05).Expression of TFF2 were correlatedwith age(P＜0.05).Thepositive rate of HPV16/18 was significant difference between cervical carcinomas,CIN and normal tissues(P＜0.05),and it was not associated with age,tumor grades,clinical stages and lymph node metastasis(all P＞0.05).The expression of CDK1 was not associated with TFF2 and HPV16/18(all P＞0.05).The expression of HPV16/18 was associated with TFF2(rs=-0.500,P＜0.05).Conclusions The results suggest that in tissues of CIN and cervical carcinomas in TFF2 and CDK1 expression changes and HPVl6/18 infection interact with each other,which can effect the progression of CIN and carcinogenesis of cervical carcinomas.

Objective:To explore the expression of Toll-like receptor(4TLR4),nuclear factor -kappaB p65(NF-?Bp65) and tumor necrosis factor-alpha(TNF-?)in placenta of preterm birth of infected mouse model,as well as intervention of N-Acetylcysteine(NAC) in order to develop a novel strategy for prevention from premature birth.Methods:Expression of mRNA and protein of TLR4,NF-?Bp65 and TNF-? in the mouse placenta of premature birth was analyzed by using RT-PCR and immunohistochemistry.LPS injection was taken to develop the infectious mouse mode,and the incidence of premature birth was observed.The regimen of NAC intervention was performed and its modulation for placenta expression of TLR4,NF-?Bp65 and TNF-? was investigated.Results:It was shown that TLR4,NF-?Bp65 and TNF-? were expressed at a low level in control groups and in LPS model groups,their expressions were significantly increased when compared with the control groups(P

Objective:To investigate the expression of minichromosome maintenance proteins 4(MCM4)and cell division cycle 6(CDC6)in uterine cervical carcinomas and its relationship with human papilloma virus(HPV)16/18 infection.Methods:The expression of MCM4 and CDC6 was examined in 50 squamous cell carcinoma specimens,20 cervical intraepithelial neoplasia(CIN)Ⅱ-Ⅲ specimens,20 CINⅠ specimens,and 20 normal cervical tissues by immunohistochemical method.The infections of HPV type 16,18 DNA were determined by PCR.Results:(1)The expression of MCM4 and CDC6 in uterine cervical carcinoma tissues was significantly higher than that in the CIN specimens and normal cervical tissues(Both P0.05).(2)The positive rates of(HPV)16/18 were significant different between cervical carcinomas,CIN and normal tissues(P0.05).(3)MCM4 expression were positively correlated with the expression of CDC6 in uterine cervical carcimonas(r=0.390,P

Objective To screen the hierarchical,mutually supplementary Appropriate Technology in Health suitable for the promotion and application in the county and township two-level medical institutions in Chongqing for gradually establishing and per-fecting the grading and classification of Appropriate Technology in Health database,and expanding Appropriate Technology in Health reserves.Methods Using Lv Yalan[1] assessment index system of appropriate health technology,then the experts′scoring method in the field was adopted for evaluating and screening the Chongqing Municipal Science and Technology Proj ectbased on stratification of diagnosis and treatment of disease complementary appropriate technology research,evaluation and integration dem-onstrationAppropriate Technology in Health group[2].Results The comprehensive scores of various techniques were high,in which the lowest score was 94.61 points,including:the drug seretide inhalation technique 98.24 points,the drug salbutamol inhala-tion technique 98.56 points,inhaled medications to control asthma technique 97.08 points,the drug spiriva(tiotropium)inhalation technique 97.97 points,6-min walk test 96.31 points,pulmonary ventilation function inspection technique 95.61,summary pulmo-nary function testing technique 94.59,children bronchial get up early screening technique 94.59,child bronchial asthma detection technique-C-AT(children′s asthma control test)evaluation form 96.94.Conclusion According to the inclusion criteria for Appro-priate Technology in Health and the experts′scores,9 items of Appropriate Technology in Health included by preliminary screening are selected for putting in storage,combined with the health resources and the prevalence rate in the demonstration counties and townships,these 9 items of technology may be promoted and applied.

Objective To explore the neurasthenia situation of the population attending the postgraduate exam in medical class and its influencing factors .Methods The random cluster sampling method was adopted .The influencing factors were per‐formed the χ2 test and Logistic regression analysis .Results According to the inclusion criteria and exclusion criteria ,36 positive ca‐ses were screened out ,accounting for 8 .89% of participants ,which was higher than the value of the general population ;the statisti‐cally significant differences were noted in the factors of the confidence of attending the postgraduate exam ,stress ,adjusting ability , interpersonal situation ,reading duration ,sleep ,nutrition and dietary (P<0 .05) .Conclusion Attending the postgraduate exam such highly intensitive mental activity is the important reason causing neurasthenia among the population attending the postgraduate ex‐am ,which mainly display in the aspects of stress size ,bearing ability facing stress and regulation .

Objective To investigate the relationship between expression of survivin and CD44v6 and HPV16/18 infection in uterine cervical carcinogenesis.Methods Using Streptavidin-Peroxidase(S-P) immunohistochemical technique,the authors examined the expression of survivin and CD44v6 in samples.The infection of HPV type 16,18 DNA was determined by PCR.Results There were significant differences for survivin and CD44v6 between carcinomas,CIN and normal cervices(P

Objective To evaluate the association between hemoglobin concentration and stroke severity on admission in ischemic stroke without diabetes. Methods Based on the China National Stroke Registry,the information of acute stroke patients were collected by trained research coordinators and investigators with the methods of review clinical records or interview. Demography, disease history, behavior and habits, hemoglobin concentration,and NIHSS score on admission were collected in this study. The iIncluded patients with the integri?ty of the information of non diabetes,3 h to the hospital,no gastrointestinal bleeding and Hb concentration and NIHSS score at admission. Hemoglobin concentration was classed according to quintiles and the outcome was grouped into ≤3 and >3 groups. The method of logistic regression was used to explore the association between hemoglobin and NIHSS. Results A total of 1 419 individuals was included in this study,including 883 males and 536 females. The mean age was 67. 24±12. 46 years old and the proportion of NIHSS>3 was 67. 51% (958/1419). With respect to non?minor stroke (NIHSS>3),the odds rations and 95% confident intervals of patients with hemoglobin ≤121. 0 g/L(Q1),>122. 1-≤132. 0 g/L(Q2),>141. 0-≤152. 0 g/L(Q4),≥152. 1 g/L (Q5) were 1. 84(95%CI 1. 21-2. 79,P=0. 004),1. 24(95%CI 0. 83-1. 86,P=0. 294),1. 32(95%CI 0. 88-1. 96,P=0. 178) ,1. 52( 95%CI 1. 01-2. 28,P=0. 044) respectively,compared with hemoglobin between 132. 0 and 141. 0 g/L( Q3) . Conclusion Stroke severity is associated with lower and higher hemoglobin concentration in acute ischemic stroke without diabetes.

Objective To establish a scientific and practical principle , grading standard and reasonable statistical method for evaluating the protective effect of health food to gastric mucosal injury , based on general pathology and histopathological diagnosis .Methods A methodological study was conducted on rat model of acute gastric mucosa injury induced by alcoholic through comprehensive analysis and comparing shortcomings of the current standard evaluation method, and methods of semi-quantitative analysis and corresponding information statistic processing were based on characteristics of the lesion and principles of pathology .Results Gross pathological evaluation of gastric mucosa lesion was based on the area occupied and proportion in the whole gastric mucosa .Histopathological diagnosis was based on the mucous layer depth of lesion as main determination point and other lesions as reference factors .Grades of lesion were divided into no abnormality (0), mild lesions (1 point), moderate lesions (2 points), and severe lesions (3 points). Ridit statistical method was used for pathological analysis of semi-quantitative results .Conclusion A scientific and feasible evaluation method for the protective effect of health food to gastric mucosal injury was provided from the aspects of gross pathology , histopathological evaluation method , data processing method and result determination .

Objective To explore the relationship between life events and depression of patients with primary sjgren's syndrome (PSS).Methods 80 patients with PSS complicating depressive disorder were tested by self-rating depression scale(SDS) and life event scale (LES),and compared with the control group including 80 PSS patients with non-depressive disorder matched with age and sex.Then the correlation between SDS and LES were analyzed.Results The total score of life events (32.72 ± 13.93),the score of negative life events (24.36 ±11.24),the score of SDS(58.60 ± 16.78) and the frequency of life events(91.3％) in the research group were higher than those in control group(P ＜ 0.05),but the positive life events score (7.15 ± 5.84) was lower than that in control group (9.76 ± 7.29),and there was statistically significant difference between the two groups (P ＜ 0.05),there were more negative life events,especially on the aspects of family,work and study in the research group.There was positive correlation among the negative life events score,total score of life events and SDS score in the research group(P＜ 0.01),meanwhile,negative correlation was revealed between the score of positive life events and SDS score in the research group(P ＜ 0.05).Conclusion Patients with PSS complicating depressive disorder experienced more negative life events which play an important role in depression of patients with PSS.