Objective To study the efficacy of stageⅡ/Ⅲcolon cancer postoperative adjuvant chemotherapy and survival time.Methods 108 stage Ⅱ/Ⅲ colon cancer patients with postoperative adjuvant chemotherapy were selected as the study group,and in the same period 52 stage Ⅱ/Ⅲ colon cancer patients with only simple surgery treatment were selected as the control group.The adjuvant curative effect of the two groups were analyzed and the sur-vival time was followed up.Results The patients of the two groups were followed up for 3 years.The median disease-free survival time of the study group was 18 months,disease-free survival rate was 69.44%,26 cases in recurrence and transfer,accounted for 24.07%,and 7 cases(6.48%) died;The median disease-free survival time of the control group was 12 months and the disease-free survival rate was 46.15%,18 cases in recurrence and transfer, accounted for 34.62%,and 10 cases(19.23%) died.The median disease-free survival,recurrence and transfer rate of the control group were statistically significant better than those of the control group(χ2 =8.07,4.74,all P<0.05).Postoperative adjuvant chemotherapy in patients with adverse reaction was mainly for nausea and vomiting,loss of appetite,pigmentation and hair loss up to see, but were comparatively light, and very little serious most patients could be tolerated.Conclusion The postoperative adjuvant chemotherapy for patients with stageⅡ/Ⅲcolon cancer can reduce postoperative recurrence,metastasis,improve patients'survival time,prolong patient life.

Two new single chain ribosome—inactivating proteins,polygonin2 from the roots ofPolygonatum odoratum and momordin2,from the seeds of Momordica charantia,inhinited pro-teib synthesis potentially in rabbit reticulocyte lysate,but were relatively low toxic to Molt—4 cells.polygonin2 and momordin2 were conjugated to H65 monoclonal antibody that recognizedhuman T lymphocyte CD5 surface antigen using a heterobifunctional crosslinking reagent 2—iminothiolane.The resulting immunotoxins,referred to as H65—PGN2 and H65—MOR2,showed potent specific cytotoxicity to target cell Molt—4 and human peripheral blood T lympho-cyte,but had no effect on human hematopoietic cells.Injection of Molt—4 cells s.c.into Balb/cnu/nu mice developed into a solid tumor.Administration of the H65—PGN2 or H65—MOR2immunotoxins i.p.10 ug per mice at 48—h intervals suppressed tumor growth specifically.Theresulting showed that clinical studied were warranted to use these conjugates for the therapy ofautoimmune diseases as well as the ex vivo purging of T lymphocyte in ABMT.

Objective:Offer practical experience and theoretical basis for the popularization of Country Feasible Medical Technology(CFMT)in our nation,and explore the best mold and mechanism for popularization of CFMT at the actual situation of our nation,especially to the western rural areas.Methods:We launch the popularization and demonstration of CFMT in two countries of Chongqing City.Results:In 2005 and 2006,we adopted different modes of popularization about CFMT,and obtained different effects;besides,the CFMT reduces the difficult and expensive condition of seeing a doctor to some extent,and wins wide welcome and praise from health workers and masses.Conclusions:For the popularization of CFMT in western rural areas,the best mold is 'government-control & market-guidance' Mold.And the most suitable mechanism is a synergistic effect of many mechanisms,in which Consultation & Combined Action mechanism,Social Mobilization mechanism,Proper Financial Compensation mechanism,and Restriction & Inspiritment mechanism is the four main mechanisms.Besides,to summarize experiences the participation of college and scientific research institute is needed in the whole process of research project.

Objective Analysis of K.pneumoniae infection in the Pediatrics and the Neonatology clinical characteristics of the resistance,that can guide and use medicine clinically rationally.Methods The sensitiveness test and ESBL detection to the 20 kinds of antibiotics were examined by Automatic Instrument bacterial identification and susceptibility testing ;ESBL positive strains with the standards disk diffusion method phenotypic confirmatory test.Results The 207 strains of K.pneumoniae from fecal specimens were 77.3% and from respiratory specimens were 18.8%.Imipenem and meropenem sensitivity 100%,followed by compound piperacillin,ciprofloxacin,compound amoxicillin and cefoxitin,that sensitive rates were 98%,97%,96% and 96%,;ceftazidime,cefepime,cefotaxime lower sensitivity were 75%,75%,78%.50 ESBL-producing strains,accounting for 24.2%.Conclusions K.pneumoniae have different levels of the drug resistance expcept Imipenem and meropenem,and the multi-drug resistant strains ratio and the positive rate are high.The sensitiveness of penicillin compound containing enzyme inhibitor is second only to the two described above,and they are economy and effective.Considering by medicine economics,the kinds of medicine is preferred on according to the drug sensitivity.

Objective To investigate the relationship between expression of CDK1,TFF2 and HPV16/18 infection in uterine cervical carcinomas.Methods Formalin-fixed,paraffin-embedded samples including 50 cases of squamous cell carcinomas,20 cases of cervical intraepithelial neoplasia CINⅡ-Ⅲand 20 cases of CINI and 20 cases of normal cervical tissues were studied.Using Polymer DetectionSystem For Immuno-Histological Staining(PV-9000)as immunohisto-chemical detecting method.the expression of TFF2 and CDK1 in these samples were examined.The infections of HPV type 16,18 DNA were determined by PCR.Results There were significant differences for CDK1 between carcinomas,CIN and normal cervices,respectively(all P＜0.05).Expression of CDKl were correlated with tumor grades(P＜0.05).The positive expression of TFF2 in carcinomas was significantly lower than that in normal cervical tissue and CIN.There was significant differences amongthem(P＜0.05).Expression of TFF2 were correlatedwith age(P＜0.05).Thepositive rate of HPV16/18 was significant difference between cervical carcinomas,CIN and normal tissues(P＜0.05),and it was not associated with age,tumor grades,clinical stages and lymph node metastasis(all P＞0.05).The expression of CDK1 was not associated with TFF2 and HPV16/18(all P＞0.05).The expression of HPV16/18 was associated with TFF2(rs=-0.500,P＜0.05).Conclusions The results suggest that in tissues of CIN and cervical carcinomas in TFF2 and CDK1 expression changes and HPVl6/18 infection interact with each other,which can effect the progression of CIN and carcinogenesis of cervical carcinomas.

Objective To reveal the average pass rate of a general hospital's ethic review and common problems.Methods The records generated by the ethics committee of a hospital in 3 consecutive years were reviewed and analysed with ridit of CMH and Bootstrap statistical methods.Results The pass rate for drug clinical trial is 36.09%,medical instruments 18.42%,and subject evaluation 14.29%.Among the problems,those concerning informed consent take up 63.59%,of protocol 14.56%,of CRF 7.77% and of the others 22.820%.The domestic applicants had more problems than those abroad.Besides,the pass rate of drug trials decreased with year from 45.83% in 2006 to 19.44% in 2008,which was statistically significant (P=0.029).In terms of drug clinical trial,the pass rate of revised projects in foreign countries was significantly higher than the domestic ones (P=0.028).ConclusionEthics Committee in a hospital plays a very important role in clinical researches.And informed consent and research protocol are very important in the ethic review.

Objective:To explore the expression of Toll-like receptor(4TLR4),nuclear factor -kappaB p65(NF-?Bp65) and tumor necrosis factor-alpha(TNF-?)in placenta of preterm birth of infected mouse model,as well as intervention of N-Acetylcysteine(NAC) in order to develop a novel strategy for prevention from premature birth.Methods:Expression of mRNA and protein of TLR4,NF-?Bp65 and TNF-? in the mouse placenta of premature birth was analyzed by using RT-PCR and immunohistochemistry.LPS injection was taken to develop the infectious mouse mode,and the incidence of premature birth was observed.The regimen of NAC intervention was performed and its modulation for placenta expression of TLR4,NF-?Bp65 and TNF-? was investigated.Results:It was shown that TLR4,NF-?Bp65 and TNF-? were expressed at a low level in control groups and in LPS model groups,their expressions were significantly increased when compared with the control groups(P

Objective To emphasize the hazardness due to streptococcus agalactiae infecting genitourinary tract of the pregnant women,and to analyze the drug resistance to 7 kinds of antibiotics in streptococcus agalactiaes in order to guide the clinical drug usage.Methods 112 isolates of streptococcus agalactiaes were identified by API 20 STREP.The susceptibility to 7 kinds of antibiotics were performed by K-B method.Results All of the 112 strains were susceptible to penicillin,cefazolin,ceftriaxone,vancomycin.The susceptibility to erythromycin,clindamycin and levofloxacin were 39.3%,56.2%,74.1% respectively.The resistance ratio to erythromycin was 49.1% followed by clindamycin(41.1%).Conclusions Penicillin and ampicillin were the first recommended antibiotics in treating and preventing streptococcus agalactiae infection.Erythromycin and clindamycin could be selected according to antibiotic susceptibility results in case of the patient with severe penicillin allergy.It is important to detect streptococcus agalactiae in preventing its infection among pregnant women.The results of the antibiotic susceptibility test can guide the rational drug usage in clinical treatment of streptococcus agalactiae infection.

Objective To find out a new teaching pattern of Medical Statistics for medical postgraduate students.Methods To manifest the longitudinal thought of the medical research process from the teaching idea,the teaching content arrangement and the teaching method utilization.Results To discuss the superiority of the new pattern which provides the content frame and the teaching method based on the integrating theory and the practice.Conclusion The teaching reformation of Medical Statistics must be advantageous in improving the learner’s power of the medical scientific research,including selection of the scientific research topic,application of project,the quality control of the project,analysis of the material and inference of the conclusion,paper writing,self-appraisement and achievement evaluation.

Objective To established the method of increasihg the proportion of T regulatory cells(Tr) to T effector cells(Te), which could suppresses allogeneic ahtigen reaction, by in vivo of glucocorti-coid (dexamethasone, DXM) combined with IL-2. Methods After combined treatment to male C57BL/6N mice (donor) with DXM(5 mg. kg-1· d-1 ) and IL-2 (300 000 IU · mouse-1·d-1) for 3 d, spleen mono-nuclear cells were made and were carried out by flow cytometry analysis. Using the spleen cells of BALB/c mice as aliogeneic antigen to stimulate the spleen cells of male C57BL/6N mice for 7 d after combined treat-ment of glucoeorticoid and IL-2, the reaction of cell proliferation was detected. Results After the treatrment of DXM and IL-2, CD4+ CD25+ Foxp3+ Tr cells in the spleen of C57BL/6N mice increased abviously. The ratio of CD25+ Foxp3+ Tr to CD4+ T cells was 24.22%±7.60% in the group of DXM combined with IL-2, while the control group was 4.02% ±0. 84% ( P =0. 01 ). Compared with the control group (0. 14±0.01 ), the ratio of Tr to Te increased obviously in the group of DXM combined with IL-2 (0.43±0. 15 ) ( P = 0.01 ). The group of DXM combined with IL-2 also expressed more glucocorticoid-induced tumor necrosis factor receptor(GITR) and alloreaction was suppressed/n vitro obviously ( P < 0. 05 ). Conclusion DXM amplifies IL-2 induced the proportion of Tr to Te, and suppresses the cell proliferation stimulated by alloge-neic antigen.