AIM: To discuss the effect of Smart Plug canalicular plug on aqueous-deficient dryeye.
METHODS:Forty-eight cases of aqueous-deficient dry eye patients in our hospital from May 2012 to April 2013 were selected for the study. After treated by Smart Plug canalicular plug, postoperative clinical efficacy, foundation Schirmer I test ( SIt) , tear film break-up time ( BUT ) , corneal fluorescein staining ( FL ) changes were observed.
RESULTS: Forty-eight patients were cured, 31 cases were markedly effective(65%), effective 14 cases (29%), invalid in 3 cases (6%), the total effective rate was 94%. Before treatment, SⅠt, BUT, and FL was ( 3. 49±1. 24 ) mm/5min, ( 3. 15±1. 07 ) s, and ( 2. 52±0. 11 ) points, respectively. After treatment, SIt, BUT, FL were significantly improved compared with before treatment, the difference was statistically significant (P<0. 05). One patient had postoperative foreign body sensation, 8h after shedding emboli; One patient granulation tissue after surgery 8mo, canalicular plug off. The remaining cases had no inferior lacrimal duct infection or granuloma.
CONCLUSION: Smart Plug canalicular plug is an effective treatment for aqueous-deficient dry eye, can effectively relieve symptoms, worthy of promotion.

AIM: To observe the clinical effect of the neotype lacrimal drainage tube(RS model)for the treatment of lacrimal duct obstruction.

METHODS: It was a retrospective case series study. Totally 75 cases(93 eyes)of lacrimal duct obstruction were enrolled in the study and randomly divided into two groups: treatment group(50 eyes)and control group(43 eyes). The treatment group was implanted the neotype lacrimal drainage tube(RS model). The control group underwent the implantation of annular silicone tube and their efficacy were analyzed.

RESULTS: Followed up for 3-12mo after extubation, the cure rate and total effective rate of the treatment group were 90%, 96% respectively, and the control group of it were 72%, 84%.The difference between the two groups was statistically significant(P<0.05). The total rate of intraoperative and postoperative complications was 8% in treatment group, 35% in control group respectively, the rate of the control group was obviously higher than that of the treatment group, there was significant statistical difference between the two groups(P<0.05).

CONCLUSION: The implantation of the neotype lacrimal drainage tube(RS model)is safe,fast and simple, easy to operate and extubate easily with good clinical effect in the treatment of lacrimal duct occlusion.

AIM: To observe the clinical effects of modified endoscopic dacryocystorhinostomy(EDCR)for chronic dacryocystitis and try to find out an effective adjuvant method to improve the long-term effect of the modified surgery.

METHODS: Totally 136 cases(158 eyes)of chronic dacryocystitis were enrolled in the study and randomly divided into two groups:treatment group(80 eyes)and control group(78 eyes).The treatment group underwent the modified EDCR(intraoperative mytomycin C combined with silicone tube implatation and the use of tobramycin and dexamethasone eye ointment).The control group was only applyed mytomycin C during EDCR.The postoperative follow-up period was for 3-12mo.

RESULTS: At 6mo postoperatively,the cure rate and total effective rate of the treatment group were 95.0%, 98.8% respectively,and the control group of it were 75.6%, 93.6%.The effective rate of the treatment group was higher than that of the control group significantly(χ²=11.90,P<0.05).

CONCLUSION: The modified EDCR is a new surgical method which can prevent postoperative cicatricial adhesion and elevate surgical effective rate, and it has clear field, minimal invasion, quick recovery, exact effect and less recurrence.

Based on the deletion information of high virulent PRRSV genome, 3 oligonucleotide primer were designed and synthesized. Specific and sensitive reverse transcription-PCR (RT-PCR) assays were de-veloped for the detection of high virulent PRRSV. The sensitivity and specificity of RT-PCR assays were evaluated, the results showing that the detection limit of the assay was found to be 0.265 pg of tissue total RNA, and the protocol have no cross-reaction with classical swine fever virus, porcine circovirus type 2,pseudorabies virus, streptococcus, haemophilus parasuis and Escherichia coli. Then 36 cell cultures, two PRRSV live vaccine strains and 184 clinical specimens from 52 farms were tested. Five PRRSV field iso-lates were the high virulent PRRSV; two PRRSV live vaccine strains from normal PRRSV, and 123 speci-mens from 42 farmer were positive (only 1 specimen was normal PRRSV). This RT-PCR method proved to be accurate differential diagnosis of the high virulent PRRSV and normal PRRSV with the characteristics of rapidity, sensitivity and specificity, and has a strong clinical relevance.