Antigenic analysis of 10 strains of Coxsackievirus type A 24 (CA24) was carried out by monoclonal antibodies (McAbs) prepared with a China isolate of Coxsackievirus A 24 variant (CA24v).8 McAbs which were positive in indirect immunofluorescent test reacted to 9 CA 24 v detected. Of them, 4 McAbs could react to both CA 24v and CA 24 prototype (Joseph strain),and the others did not react to CA 24 prototype. 5 McAbs possessing virus-neutralizing activity did not neutralized CA 24 prototype (Joseph strain)and only one of them could neutralize EH 24/70 strain weakly, three of them neutralize 2 isolate from Japan in 1985-86 (J140/85 and J 60/86) and one isolate Fujian of China in 1986. However, all 5 McAbs above could neutralize isolate from Henan and Shanghai of China in 1986 as well as Beijing, Liaoning and Fujian of China in 1988.

Objective To study the clinical effect and surgical treatment of intestinal obstruction.Methods From January 2015 to December 2016,45 cases with intestinal obstruction who admitted to the Department of General Surgery of Taizhou First People's Hospital were selected in the research.According to the single blind randomized grouping method,the patients were randomly divided into two groups.22 cases in the control group were given conservative treatment,23 cases in the observation group received surgery.The clinical total effective rate ,abdominal pain relief time,recovery time,gas-liquid plane disappeared time ,hospitalization time and the incidence of complications were compared between the two groups.Results The clinical total effective rates of the two groups were 90.91%, 95.65%,respectively,the difference was not statistically significant (χ2＝0.407,P ＞0.05).The abdominal pain relief time,recovery time, gas -liquid plane disappeared time , hospitalization time in the observation group were significantly shorter than those in the control group (t＝4.373,4.332,3.372,3.529,all P＜0.05).The incidence rate of complications of the observation group was significantly higher than that of the control group (χ2＝4.294,P＜0.05).Conclusion Surgical treatment has significant clinical effect for patients with intestinal obstruction ,which can effectively promote the remission of symptoms ,shorten the treatment time,but with more complications ,so it need to be cautious in the choice of treatment.

OBJECTIVETo observe the effects of electroacupuncture (EA) with different frequencies based on the western conventional treatment and rehabilita tion training for stroke dysphagia.

METHODSSixty patients with dysphagia after stroke were randomized assigned into a low frequency (2 Hz) group and a high frequency (100 Hz) group, 30 cases in each one. Basic treatment was applied in the two groups. The acupoints were Fengfu (GV 16) and Lianquan (CV 23). EA with continuous wave and tolerant intensity were connected for 30 min, once a day for 14 days. The main index was video fluoroscopic swallowing study (VFSS) to detect passing time at oral stage, pharynx delay time at pharyngeal stage, passing time at pharynx stage. The second indexes were water swallow test rating and standardized swallowing assessment (SSA). The clinical effect was evaluated.

RESULTSAfter treatment, the passing time at oral stage, pharynx delay time at pharyngeal stage, passing time at pharynx stage were lower than those before treatment in the two groups (all<0.05), and the results in the low frequency group were better (all<0.05). After treatment, the water swallow test rating improved in the two groups (both<0.05), and that in the low frequency group was better (<0.05). The SSA score decreased in the two groups (both<0.05), and the improvement in the lower frequency group was superior to that in the high frequency group (<0.05). The total effective rate in the low frequency group was 93.3% (28/30), which was better than 66.7% (20/30) in the high frequency group (<0.05). .

CONCLUSIONThe effect of EA with lower frequency is better than that of EA with higher frequency for stroke dysphagia.