Objective To compare the clinical effects between rivaroxaban and warfarin on preventing deep vein thrombosis (DVT) in elderly hip fracture patients with diabetes mellitus (DM) after operation. Methods One hundred and sixty-eight elderly hip fracture patients with DM were randomly divided into the observation group and the control group. The observation group (88 cases) were treated with rivaroxaban (10mg, po, qd) after surgery, and the control group were treated with warfarin and low molecular weight heparin calcium for 14 days. Respectively after 7, 14, 21 and 28 days, the color doppler ultrasonography was used to examination vein blood flow and the DVT of affected lamb. In addition, the levels of D-dimer and bleeding were observed during treatment. Results The incidence of DVT in observation group (2 cases, 2.23%) was significantly lower than that in control group (7 cases, 8.75%), and DVT of control group was happened on 7th day after surgery. In addition, the level of D-dimer on 7th day post-surgery in observation group significantly decreased, while it significantly decreased on 14th day post-surgery in control group (P<0.05). However, the incidence of bleeding events in observation was less than that in control group (P<0.05). Conclusion Rivaroxaban can be more effective compared with warfarin in preventing diabetes mellitus elderly hip fracture patients with postoperative DVT formation and significantly reduce the bleeding risk, with more safe and convenient.

Objective To provide a express plasmid carrier platform that can be in common use for preparation of RNase-resistant RNA standards and controls. Methods A cDNA fragment of MS 2 phage RNA genome, which encodes coat protein and maturase protein, and expression vector pET28b DNA are ligated together with T 4 DNA ligase after digested with HindⅢ and EcoR Ⅰ restriction nucleases. Then, a new expression plasmid carrier pI NCCL is contructed。 The prokaryotic expression was carried out by transform pI NCCL into BL21-DE3 E。Coli. Results A new expression plasmid carrier pI NCCL is contructed successfully. RNase-resistant virus-like particles were obtained after prokaryotic expression of pI NCCL. Conclusion The expression plasmid carrier pI NCCL contructed and prokaryotic expression system can be used as a express plasmid carrier platform that can be in common use for preparation of RNase-resistant RNA standards and controls.

Objective To observe the effect of Sijunzi Decoction(SD) and Huangqi Sijunzi Decoction(HSD) on mitochondrial succinate dehydrogenase,cytochrome oxidase activity of liver in rats with spleen asthenia,thus to reveal the pathogenesis mechanism of spleen asthenia from energy metabolism,and to clarify the therapeutic mechanism of spleen-invigorating and Qi-tonifying herbs.Methods Forty SD rats were randomly divided into 4 groups:normal control group,spleen-asthenia model group,SD(10.5 g/kg) group and HSD(18 g/kg) group,10 rats in each group.Except that the normal control group,the rats in the other groups were fed with half-full diet once every other day and with gastic gavage of Xiaochengqi Decoction 60 g/kg every day to establish animal model of spleen asthenia.After 15 days,the model rats were administered the corresponding drugs according to the experimental design for 4 continuous weeks.The changes of mitochondrial succinate dehydrogenase,cytochrome oxidase activity of liver were measured,and the general status of the rats were observed.Results Compared with the normal control group,the level of mitochondrial succinate dehydrogenase and cytochrome oxidase activity in liver of spleen asthenia group were decreased obviously(P

Objective To evaluate the impact of preoperative prognostic nutritional index (PNI) on surgical outcomes and long-term survival of elderly patients with gastric cancer.Methods From Jan 2007 to Dec 2012,the clinicopathological data of 165 elderly patients (age ＞ 65 years old) with gastric cancer undergoing radical gastrectomy in the Second Hospital of Tianjin Medical University were analyzed retrospectively.PNI value was calculated by serum albumin (g/L) + 5 × lymphocyte count (× 109/L).The receiver operating characteristic (ROC) curve and Youden index was used to determine the cut-off value of PNI.Patients were then divided into low PNI group and high PNI group based on the cut-off value.The two groups were compared according to clinicopathological characteristics,postoperative complication and longterm survival.Results The mean PNI value was 46.1-± 5.3,When the PN1 was 46.4,the Youden index was maximal,with a sensitivity of 78％ and specificity of 59％.There were 79 patients in the low PNI group and 86 patients in high PNI group.No significant correlations between PNI and gender,age,BMI,pathological type,tumor diameter,extranodal metastasis and postoperative morbidity were observed.There were significant difference in the depth of invasion (x2 =6.732,P =0.009),N stage(x2 =13.191,P =0.004),TNM stage(x2 =12.137,P =0.000) and postoperative hospital stay (t =4.595,P =0.000).By Kaplan-Meier method,PNI (P =0.000),depth of invasion (P =0.002),N stage (P =0.000),TNM stage(P =0.000) and extranodal metastasis (P =0.031) had significant impacts on overall survival.PNI (P =0.016)and N stage (P =0.018) were independent factors for predicting overall survival rate.Conclusions PNI helps in evaluating preoperative nutritional status and postoperative prognosis for elderly patients with gastric cancer.

Objective To study the correlation between status of methylation of DKK3 gene promoter and expression of its mRNA in gastric cancer.Methods Methylation of DKK3 gene promotor and its mRNA expression in 76 gastric cancer specimens and paired adjacent non-cancerous tissues were detected by methylation-specific PCR(MSP) and RT-PCR respectively.Results The expression of DKK3 mRNA in gastric cancer tissues was lower than that of adjacent non-cancerous tissues (43％ vs.60％,x2 =5.663,P =0.017).29 cases had methylation of DKK3 gene promoter for adjacent non-cancerous tissues.In gastric cancer tissues,however,44 cases had methylation of DKK3 gene promoter (38％ vs.58％,x2 =19.405,P =0.000).There were significant difference in depth of invasion (P =0.014),TNM stage (P =0.032),pathological grading (P =0.016) and lymphatic metastasis (P =0.007).There was distinct negative correlation between methylation of DKK3 gene promoter and expression of its mRNA (x2 =64.286,P =0.000).Conclusions Abnormal methylation of DKK3 gene promoter is an important mechanism of its inactivation,and might play an important role in carcinogenesis and progression of gastric cancer.

Objective To study the expression of DKK3 protein and mRNA levels in gastric cancer and its correlation with the clinical prognosis of gastric cancer.Methods The expression of DKK3 protein in 158 gastric cancer specimens and paired adjacent non-cancerous tissues were detected by immunohistochemistry.The relationship between the protein expression and clinicopathological features was analyzed.Immunoblotting and real time quantitative PCR were used to examine the expression of DKK3 protein and mRNA levels in 76 paired fresh gastric cancer and adjacent non-cancerous tissues.Results The expression of DKK3 in gastric cancer tissues was lower than that in adjacent non-cancerous tissues (40.5％ vs.55.1％,x2 =41.442,P =0.000).DKK3 protein and mRNA levels in 76 cases were lower than that in adjacent non-cancerous tissues (1.096 ± 1.110 vs.3.476 ± 1.119,t =3.332,P =0.017;1.136 ± 1.013 vs.3.314 ± 1.105,t =2.673,P =0.022).There were close correlations between DKK3 expression and depth of tumor invasion (P =0.006),TNM stage (P =0.005) and lymphatic metastasis (P =0.009).The expression of DKK3 in gastric cancer was positively related with overall survival rate (47.4％ vs.28.0％,P =0.011),as an independent prognosis predictor (P =0.016).Conclusions DKK3 is an independent prognostic factor of gastric cancer,its decreased expression is significantly related with shortened survival time of gastric cancer patients.