1.Assessment of the value of academic libraries
Wenxiu GUO ; Xiaojing SHI ; Ruizhen KONG ; Yongxu YUAN
Chinese Journal of Medical Library and Information Science 2015;(1):33-37
Assessment of the value of academic libraries is an effective means to describe the value of academic libraries themselves. The dimension and factors for the assessment of the value of domestic and foreign academic li-braries were analyzed according to the assessment of the value of Shanxi Medical University Library, and the frame-work for the assessment of the value of academic libraries was proposed in terms of the impact of academic libraries on higher education.
2.Upregulating the renin-angiotensin system in bone marrow mesenchymal stem cells by hypoxia
Rongrong XIAO ; Jinghong GAO ; Yue FAN ; Lu ZHOU ; Ruizhen SHI ; Qingping LI
Journal of Medical Postgraduates 2015;(2):123-126
Objective The renin-angiotensin system ( RAS) is involved in myocardial anoxic injury .This study aimed to in-vestigate the expressions of AT 1-R, AT2-R, and angiotensin-converting enzyme ( ACE ) in bone marrow mesenchymal stem cells (MSCs) under hypoxia. Methods Rat MSCs were isolated, cultured, and identified with CD29 and CD11b/c antibodies.The is-chemic injury model was established by exposing the MSCs to hypoxia and serum deprivation ( Hypoxia/SD) for 24 hours, while the control cells were cultured in L-DMEM with 10%FBS.The vitality and apoptosis of the cells were detected by trypan blue staining , CCK8 assay, and Annexin V-FITC staining.The mRNA and protein expressions of AT 1-R, AT2-R, and ACE were determined by real-time quantitative PCR and Western blot , respectively. Results The positive rate of CD29 was >97%and that of CD11b/c was <1% in the MSCs.Compared with the control group, Hypoxia/SD significantly increased the rate of cell apoptosis ([6.73 ±0.78]%vs [19.93 ±4.92]%, P<0.01), decreased the rate of cell viability ([78.49 ±4.94]%vs [37.33 ±2.91]%, P<0.01), and up-regulated the mRNA and protein expressions of AT 1-R, AT2-R, and ACE. Conclusion Hypoxia/SD activates the RAS in MSCs and improves the protective function of the cells against myocardial anoxic injury .
3.The investigation of high risk type human papillomavirus prevalence and incidence rates of cervical intraepithelial neoplasia in Shenzhen women with different screen
Ruifang WU ; Qingzhi ZHOU ; Zhihua LIU ; Ruizhen LI ; Chunyan HAN ; Jufang SHI ; Junhua LI ; Lijie ZHANG ; Ni LI ; Youlin QIAO
China Oncology 2006;0(09):-
Background and purpose:HPV infection is known as the primary cause of cervical cancer worldwide To investigate high risk type human papillomavirus (HPV) prevalence and incidence rates of cervical intraepithelial neoplasia (CIN) and their screen risk factors in women with different methods of screening.Methods:1137 residents, workers and service women aged 15-59 from Shenzhen city were investigated for cervical cancer in an epidemiology screening study.The high risk types of human papillomavirus of liquid-based cytology samples were tested by hybrid capture 2 (HC-Ⅱ) and liquid-based cytology test (LCT) was also performed at the same time. Women for HPV-positive with LCT ≥ atypical squamous cells of undetermined sign (ASCUS) or HPV-negative with LCT ≥ low grade squamous intraepithelial lesion (LSIL) were biopsied in colposcopy and then were examined by pathology. All data was managed by Foxbase. ?2 test and unconditional Logistic regression model were used for data analysis by SPSS 10.0.Results:1137 women were eligible in our research, the overall rates of HPV infection was 14.0%. HPV detection rates in residents, workers and service women were 14.1%,9.2%,18.9% respectively. HPV detection rates in workers group was significantly lower than that of service women and residents (P