The art of warm disease of TCM not only has abundant clinical experience,but also has a profound theoretical thinking,and it starts with its unique concept category and logical system,and riches and enriches,updates and develops on the basic of this,and goes along the road of independent development,has a distinct characteristics.This paper preliminarily studies on wei qi ying and xue of the art of warm disease of TCM from meaning,development of history,nature and action,et al.And it provides references of ideas and methodologies for the development and innovation of the art of warm disease of TCM.

OBJECTIVE To investigate the distribution of familial hepatitis B virus (HBV) genotypes and analyze the relationship between genotypes and clinical outcome. METHODS A total of 136 cases of familial hepatitis B were involved in this study,including 25 cases of asymptomatiec HBV carriers (ASC),85 cases of chronic hepatits B (CHB),24 cases of liver cirrhosis (LC) and 4 cases of hepatocellular carcinoma (HCC). Nested polymerase chain reaction-restriction fragment length polymorphism (ntPCR-RFLP)was used to identify the genotypes of HBV. RESULTS There were 124 cases with hepatitis genotype C,7 cases with genotypes B+C mixed,3 cases with genotype B and 2 cases that did not conformed to be any of genotypes from A to H. Genotype C in the HCC was predominant genotype (100%),next in the LC(96%),CHB (95.3%) and ASC(66.7%).Compared with genotype C,genotypes B and B+C only existed in ASC and CHB,the proportion was 9.5% vs 23.8% and 1.2% vs 2.3%,respectively. There were no other HBV genotypes from A to H seen in familial hepatitis B in Shanxi province. CONCLUSIONS HBV genotype C is the major one in familial hepatitis B in Shanxi. There are a few genotypes B and B+C existed. Genotype C induces a relatively bad prognosis.

Objective To explore a modality of ambulatory nursing care applied to the elderly patients with severe trigeminal neuralgia treated by radiofrequency thermocoagulation.Methods42 patients were equally assigned into 2 groups: group A(n=21) aged from 40～60, and group B(n=21) aged from 80～92. Pertinent nursing measures were assessed for risks of ambulatory surgery, including psychological care before surgery and intensive observation during surgery. The pain was assessed with Visual Analogue Scale (VAS) between the groups and before and after surgery, as well as complications.ResultsNo significant difference of the VAS scores was found between the groups before and 2 weeks after surgery (P>0.05), while the score of VAS decreased in two groups 2 weeks after surgery(P<0.01). No severe complication occured in the groups after surgery.ConclusionIt is safe and effective to apply the modality of ambulatory nursing care for the elderly patients with severe trigeminal neuralgia treated by radiofrequency thermocoagulation.

Molecularly imprinted polymers for dimethoate recognition were synthesized by the precipitation polymerization technique using methyl methacrylate (MMA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker. The morphology, adsorption and recognition properties were investigated by scanning electron microscopy (SEM), static adsorption test, and competitive adsorption test. To obtain the best selectivity and binding performance, the synthesis and adsorption conditions of MIPs were optimized through single factor experiments. Under the optimized conditions, the resultant polymers exhibited uniform size, satisfactory binding capacity and significant selectivity. Furthermore, the imprinted polymers were successfully applied as a specific solid-phase extractants combined with high performance liquid chromatography (HPLC) for determination of dimethoate residues in the cucumber samples. The average recoveries of three spiked samples ranged from 78.5% to 87.9% with the relative standard deviations (RSDs) less than 4.4% and the limit of detection (LOD) obtained for dimethoate as low as 2.3μg/mL.

ObjectiveTo investigate the status and explore methods to improve the quality of diabetic education for young and middle-aged patients.MethodsOutpatients took part in diabetic education program and young and middle-aged diabetic patients that visited the eastern district of affiliated hospital of Qingdao university medical college from May 1st,2010 to Oct.30th,2010 were investigated with questionnaire for their diabetic education status and demands.ResultsA total of 381 patients were enrolled in this study.59.1％ clinical diabetic education program participants were aged 60 or older and only 40.9％ were aged 20 to 59 years,of whom 66.7％ had been diagnosed with diabetes more than 5 years and only 33.3％ had been diagnosed with diabetes less than 5 years.According to the survey of 101 young and middle-aged diabetic patients,only 16.8％ patients younger than 60 took part in different kinds of diabetic education programs but about 95％ young and middle aged patients wished to participate in diabetic education programs.ConclusionYoung and middle-aged diabetic patients have received much less diabetic education than elderly patients.Diabetic education for young and middle-aged patients needs to be strengthened urgently.

Objective To analyze and compare the clinical application values of procalcitonin (PCT),leukocyte count (WBC) and C-reactive protein(CRP) in elder patients with infection.Methods In patients(age≥ 65 yrs,axillary temperature ＞38.0℃)with infection or suspected infection,PCT,WBC,CRP and other bacteriological examination were performed.The electronic medical records from the HIS system of our hospital were analyzed retrospectively in combination with medical history.Results Of the enrolled 219 patients,65 ones were in control group,48 ones SIRS,51 ones sepsis and 55 ones MODS.There was a positive correlation between the level of serum PCT and the infection degree.The Spearman correlation coefficient was 0.706 (95％CI:0.616-0.797,P=0.000).Based on the highest Youden index (sensitivity+specificity-1),the best cutoff point of diagnosis for PCT was ＞0.341 μg/L (sensitivity 84.5％,specificity 55.8％),a analysis of receiver operating characteristic(ROC) curve about PCT,WBC and CRP was carried.Area under the curve (AUC) of PCT to controlled infection was 0.916 (95％CI:0.864-0.967,P=0.000).Based on the highest Youden index (sensitivity+ specificity-1),the judging threshold of PCT to infection controlled or not was 0.73 μg/L (sensitivity 84.6％,specificity 88.0％).PCT level after treatment ＞0.73 μg/L showed the uncontrolled infection,＜ 0.73 μg/L controlled.Conclusions PCT has a higher specificity for elder patients with infection.The variation of PCT level can guide the application of antibiotics,avoid abuse and decrease the occurrence of drug-resistant bacteria.

Objective: To explore the safety and efficacy of lipoprotein apheresis (LA) in treating the patients with familial hypercholesterolemia (FH).
Methods: A total of 12 FH patients treated in our hospital from 2015-02 to 2016-10 were retrospectively studied. Based on intensive cholesterol lowering therapy with rosuvastatin (10-20) mg Qd and Ezetimibe 10 mg Qd, the patients received LA by double ifltration plasma pheresis (DFPP) via bilateral elbow central vein or femoral vein. The changes of lipid level were compared at before and after LA treatment.
Results: For pre- and immediately after LA treatment, the average total cholesterol (TC) was (9.42±3.65) mmol/L vs (2.84±0.83) mmol/L, low density lipoprotein cholesterol (LDL-C) was (7.31±3.46) mmol/L vs (1.95±0.82) mmol/L; at 1, 3, 7 and 30 days after treatment, TC and LDL-C levels showed increasing trend, while they were still lower than they were before treatment, allP<0.01. For pre- and immediately, 1 day, 3 days after treatment, the average HDL-C level was (0.96±0.31) mmol/L, (0.63±0.17) mmol/L, (0.56±0.15) mmol/L and (0.68±0.22) mmol/L respectively,P<0.05-0.01. For pre- and immediately after LA treatment, the average TG level was (1.90±0.86) mmol/L vs (0.88±0.38) mmol/L,P<0.05. Only 1 patient had the symptoms of hypotension, nausea and sweat, the patient was relieved by expectant treatment.
Conclusion: LA therapy may decrease blood levels of TC and LDL-C at short term in FH patients with good tolerance;even TC and LDL-C could slowly increase after treatment, while combining with lipid lowering therapy, it has been a safe and effective method for treating relevant patients.

OBJECTIVE: To investigate the features and correlation of nasal inverted papilloma complicated with polyps. METHOD: Twelve patients diagnosed as nasal inverted papilloma complicated with polyps were studied. The pathological specimens were taken from the surface, core and base of papilloma and sinus in every patient. Through comparing pathological findings, the features and correlation of nasal inverted papilloma complicated with polyps were evaluated. RESULT: In most cases, nasal inverted papilloma was located in the core and base of the mass, while the polyps were located in the surface of the mass. CONCLUSION Nasal inverted papilloma complicated with polyps may result from long-term inflammation that occurred in the surface of the epithelia of superficial papilloma. Therefore, the occurrence of neoplastic disease in core should be alerted in unilateral nasal polyp case.
Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Nasal Polyps ; complications ; pathology ; Nose Neoplasms ; complications ; pathology ; Papilloma, Inverted ; complications ; pathology

Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes. Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01). Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.

Objective To investigate the changes in serum HP and ADMA levels in patients with ACI and the correlation of their levels with recanalization after venous thrombolysis and poor prognosis.Methods A total of 260 ACI patients undergoing venous thrombolysis in our hospital from January 2020 to March 2023 were retrospectively recruited,and were categorized into reper-fusion group(n=196)and non-reperfusion group(n=64)based on the efficacy of thrombolysis.After a 90-day follow-up,they were further divided into good prognosis group(n=159)and poor prognosis group(n=101)according to the results of a modified Rankin scale.Serum levels of HP and ADMA at admission were compared between the two groups.Logistic regression analysis was used to analyze the risk factors for non-reperfusion and poor prognosis in ACI patients.ROC curve analysis was performed to evaluate the predictive value of serum HP and ADMA levels for non-reperfusion and the diagnostic efficiency for poor prognosis in ACI patients.Results The non-reperfusion group exhibited notably elevated serum HP and ADMA levels than the reperfusion group(2.10±0.21 g/Lvs1.29±0.31 g/L,1.68±0.19 μmol/L vs 0.69±0.11 μmol/L,P＜0.01).HP and ADMA were identified as significant risk factors for uncanalization after treatment(P＜0.01).The AUC value of their combination in diagnosing uncanalization after venous thrombolys-is was 0.869(95％CI:0.830-0.908).Furthermore,significantly higher serum levels of HP and ADMA were observed in the poor prognosis group than the good prognosis group(2.27±0.19 g/L vs 1.15±0.34 g/L,1.72±0.21 μmol/L vs 0.64±0.10 μmol/L,P＜0.01).HP and ADMA were also recognized as influencing factors for poor prognosis in 90 d after treatment(P＜0.01).The AUC value was 0.816(95％CI:0.768-0.865)when their combination was used to predict poor prognosis in 90 d after treatment.Conclusion HP and ADMA are highly expressed in the se-rum of ACI patients with failed venous thrombolysis and poor prognosis.Their combined detec-tion can effectively predict both uncanalization and poor prognosis.