Based on the statistics of the tide and key words of three magazines in 2015,a total of 30 documents and after expert consultation and analysis,the literature was divided into 10 research fields,and four aspects including correlation with teaching,talent cultivation,development of student and assessment were elaborated on to explore the current hot issues of medical education in China.In the ending part,the author summarized and put forward four shortages that covered deficiency of theoretical research,illegibility of talent orientation,teaching reform combining without actual condition and not paying attention to the development of teachers in study of medical education research,and provide suggestions and references for further research on medical education.

OBJECTIVE: To study the humoral factor of acupuncture in improving functional activities of the organism. METHODS: Neurons of the cerebral cortex from the new born rats were taken to be cultured in a strictly controlled medium. Seven to ten days later, fluorescein-molecular probe Fluo-3AM was used for staining intracellular Ca(2+). Ca(2+) concentration and its changes after application of acupuncture serum were measured by using a laser confocal microscope. Normal serum and acupuncture serum were derived from the normal control rats and the rats undergoing electro-acupuncture of "Baihui (GV 20)", "Zusanli (ST 36)", "Quchi (LI 11)" and "Sanyinjiao (SP 6)" for 2 weeks. RESULTS: After application of normal serum, the intracellular Ca(2+) concentration increased strikingly immediately at the beginning, and gradually got stable afterwards. When acupuncture serum was added to the culture fluid, Ca(2+) concentration lowered significantly. CONCLUSION: These results suggest that bioactive substances in serum collected from rats undergoing acupuncture can affect the intracellular Ca(2+) concentration of the cerebral neurons, and the humoral factor may participate in the effect of acupuncture in regulating functional activities of the organism.

Objective:To study the relationship between telomerase activity and the expression of proliferating cell nuclear antigen(PCNA) in oral squamous cell carcinoma (OSCC). Methods:Telomerase activity and expression of PCNA in 68 cases of OSCC,34 cases of epithelium immediately adjacent to carcinomas and 12 cases of normal oral mucosa were detected by TRAP-PCR-ELISA and immunohistochemical SP method. Results:The positive rate of telomerase in OSCC, the epithelium immediately adjacent to carcinomas and the normal oral mucosa was 67.65%(46/68), 8.82%(3/34) and 0(0/12) respectively. Telomerase activity in OSCC was related to the grade of the clinicopathology(P

Objective To study the relationships between tissue damage and the ability of the pancreatic cells to regenerate ,and analyze the alteration of the pancreatic cells regeneration .Methods Sixty rats were divided into two groups :impact group(the pan‐creas was injured by a BIM‐Ⅲ biotical impact machine ,40 rats) and control group(sham operated ,20 rats) .All rats were sacrificed at 6 h ,24 h ,72 h ,7 d after operation .The level of AMS ,LPS in the serum were detected by spectrophotometry ,pancreatic cells re‐generation were examined and analyzed by TUNEL staining and flow cytomertry ,and the Bcl‐2 and Bax expression were measured by Western blot .Results In the impact groups ,LPS was activated later than AMS ,and lasted persistently .The results from TUNEL stain ,flow cytometry and Western blot indicated that pancreatic trauma induces cell death and the compensatory prolifera‐tion of pancreatic cells .The characteristics of pancreatic cells regeneration in the animal model of isolated pancreatic trauma indicate that the proper remedial time is in the first 24h after the pancreatic trauma .Conclusion Detecting AMS and LPS at the same time can help us to determine the exocrine function of pancrease .

AIM: To investigate the significance of signal transducers and activation of transcription 3 (STAT3) in proliferation of rat intrahepatic biliary epithelial cell (BEC) induced by LPS. METHODS: Male Wistar rats were randomly divided into three groups: normal control, endotoxemia (LPS) group and rapamycin (RPM) group. Plasma LPS was detected by kinetic turbidimetric limulus test at 6 h, 12 h, 24 h, 48 h, and 72 h after injury. IL-6 secretion in liver homogenate was determined by ELISA. P-STAT3 expression in BEC was analyzed by laser scan confocal microscopy. Proliferation of BEC was detected by immunohistochemistry. RESULTS: Plasm LPS level was maximum at 6 h [(318?115) EU/L] after injury and descended closely to control level at 48 h [(29?11) EU/L]. IL-6 expression peaked at 12 h (653.4 ng/g?168.8 ng/g protein) and closed to control level at 72 h (0.013 ng/g?0.006 ng/g protein). In LPS group, significant direct correlation between p-STAT3 expression in BEC and IL-6 level in liver homogenate was found. BEC proliferation was induced by LPS at 12 h (5.2?0.5) and reached the maximum level at 24 h, which was higher than the control level even at 72 h, while RPM abrogated STAT3 activation and BEC proliferation induced by LPS. CONCLUSION: LPS induces liver IL-6 expression, which might activate BEC proliferation through STAT3 pathway.

Objective: To explore the application experiences and effects of emergency damage contro(lDC)treatment for severe abdomen trauma. Methods: The clinical data of 192 patients with severe abdomen trauma were selected as the object of this study. A retrospective analysis was done on therapy condition and effects of emergency damage control treatments. Results: Pre-hospital emergency care was performed in all 192 patients, damage control operations (DCO)were done in 186 cases, 184 cases entered surgical intensive care unit (SICU)for resuscitation, and 177 cases were adopted corresponding reoperations after SICU resuscitation. The abnomal DCO indices recovered gradually in the 72 hours after DCO. The cure rate was 90.7% with 174 cases, and the incidence of complication was as low as 2.3% with 4 cases after reoperations, and the 4 cases all healed by conservative treatments. Mortality rate was 6.2% with 12 cases, and it caused by associated organs injuries, severe primary disease and secondary infection of other organs. Conclusion: Emergency damage control treatment modus should be chosen positively and eligibly under indication to severe abdomen trauma patients. According to the difference of location and degree of injury, eligible therapies by stages are the key managements.

OBJECTIVETo provide the basal data for artificial cross breeding of Chinese herb Salvia miltiorrhiza from 7 provinces in China and its 4 relatives.

METHODThe pollen viability was evaluated by TTC (2, 3, 5-triphenylte trazolium chloride) test and the stigma receptivity was evaluated by benzidine-H2O2 method.

RESULTThe pollen viability of S. miltiorrhiza from 6 provinces in China and its 4 relatives deceased during time of pollen shedding. Their highest pollen viability was in 2 or 3 days after blooming. But the pollen viability of S. miltiorrhiza (wild and culture) from Hean province in China declined with time after blooming. The most obvious variation of the pollen viability was in S. miltiorrhiza from Shanxi province (RSD 71.3% ) and the least was in wild S. miltiorrhiza from Henan province (RSD 12.4%). The highest average pollen viability was wild S. miltiorrhiza (72.3%) from Henan province while the lowest was S. yunnanensis (38.8%). The stigmas of all the accessions had receptivity when blooming. The stigma receptivity of S. brevilabra was strong in 2 to 4 days after blooming, while the others had less change after blooming. The life span of pollen grains and stigmas could be maintained from 3 to 5 days.

CONCLUSIONThe optimum artificial pollination time of S. miltiorrhiza and its relatives was 2 to 3 days after blooming.

China ; Christianity ; Chromosomes, Plant ; physiology ; DNA, Plant ; analysis ; Flowers ; growth & development ; physiology ; Genetic Variation ; Genetics, Population ; Hydrogen Peroxide ; pharmacology ; Plant Infertility ; physiology ; Plant Proteins ; genetics ; Pollen ; Pollination ; immunology ; physiology ; Polyploidy ; Salvia miltiorrhiza ; physiology