Objective:To observe the effect of chest pain center management model and emergency green channel on pre-hospital rescue rate of patients with acute ST-segment elevation myocardial infarction (STEMI).Methods:Patients with STEMI admitted in Yunnan Provincial Emergency Center between January 2017 and June 2019 were selected as the study subjects. 522 patients with STEMI sent by emergency center were included in the observation group (chest pain center management model of first-aid), while 326 patients with STEMI who came to the hospital by themselves were included in the control group (emergency green channel for first-aid). The pre-hospital and nosocomial first-aid related indexes, cardiac function (assessed by Killip grade), rescue rate, hospital stay and incidence of complications were compared between the two groups.Results:The response time of visit, time of initial electrocardiogram (ECG) completion and total time of first-aid in observation group [(1.04±0.11)min, (1.56±0.25)min, (10.63±2.26)min] were significantly shorter than those in control group [(2.82±0.26)min, (5.99±1.06)min, (18.65±2.98)min, P<0.05]. The grade of cardiac function in observation group was significantly better than that in control group ( P<0.05). Compared with the control group, the observation group had higher successful rescue rate, shorter hospital stay , lower total incidence of complications [94.25% vs 42.02%, (6.09±1.02)d vs (8.92±1.65)d, 13.01% vs 32.12%, P<0.05]. Conclusions:Compared with emergency green channel, chest pain center management model can not only shorten first-aid related time of STEMI patients, but also improve their successful rescue rate, reduce incidence of complications and improve prognosis.

Objective To explore the mechanism by which urotensin Ⅱ induces hypertrophy of the cultured rat cardiomyocytes. Methods The cultured cardiomyocytes from neonatal SD rats were treated with urotensin Ⅱ, also with cyclosporine A for its blocking effect on urotensin Ⅱ induced cardiomyocytes hypertrophy. The mRNA and protein levels of ?-MHC and CaN were evaluated by real-time PCR and Western blotting, respectively. Results In the cells treated with 10-8 and 10-7 mol/L urotensin Ⅱ, the mRNA and protein levels of ?-MHC and CaN were significantly higher than that of control (P

[ ABSTRACT] Tyrosine kinase inhibitors ( TKIs) are now advocated as the first-line treatment for chronic myeloid leukemia ( CML) , but facing resistance and relapse .Leukemia stem cells ( LSCs ) are leukemia-initiating cells as the source of resistance and relapse .It is therefore important to discover the molecular biomarker of LSCs for developing anti -LSC strategies in leukemic therapy .15-Lipoxygenase (15-LO) is a key enzyme in the pathway of arachidonic acid and plays an important role in the occurrence and development of CML , which is specifically required for chronic myeloid LSCs . This review summarizes the influence of 15-LO on the chronic myeloid LSC characteristics of marked survival , self-renewal, proliferation , differentiation and apoptosis .

Objective To evaluate the serum organochlorine pesticides residues levels in breast cancer pa-tients with different estrogen receptor(ER). Methods 93 patients of breast cancer from Fengnan districtand 65 pa-tients from Tanghai county in Tangshan were selected,whose ER were detected by pathology and serum organochlo-fine pesticides (DDTs and HCHs) levels were detected and compared with gas chromatography/electron capture. Results The orders of detection rate of HCH isomer in breast cancer patients in two areas were β-HCH >δ-HCH > α-HCH >γ-HCH. The orders of detection rate of DDT isomer in breast cancer patients in two areas were PP'-DDE > PP'-DDT > OP'-DDT > PP'-DDD. The serum levels of β-HCH, PP'-DDE in breast cancer patients in two are-as were positively correlated with age (r=0.272 ,0.330 , P<0.01) or BMI (r=0.312,0.187, P <0.01, <0.05). There were statistical differences in the serum β-HCH,δ-HCH,PP'-DDE levels between the estrogen receptor posi-tive breast cancer patients and the estrogen receptor negative breast cancer patients in two areas (P<0.05 for each). Conclusion The serum organochlorine pesticides residues levels in breast cancer patients with positive es-trogen receptor are higher than that with negative estrogen receptor, implicating that breast cancer is dependent on es-trogen. The cause of breast cancer is likely due to estrogen-like effect of serum organochlorine pesticides residues.

Objective To explore the therapeutic efficacy and operation experience of Prolene Hernia System (PHS) in the tension-free repair operation of inguinal saddle hernia. Methods All 41 cases using PHS were selected as the subjects of this study and the results were analyzed. Results All patients were performed operation under the local anesthesia, and lasted from 25 min to 60 min. They had been keeping in ward for observation from 24 h to 72 h. No serotal swelling, hematoma, and incisional wound infection hap-pened after the operation. The follow-up time had been lasting from 6 months to 42 months, and none of pa-tients catehed a relapse or had the sensation of the foreign bodies. Conclusions Using PHS is safe and ef-fective in the tension-free repair operation, and it is more suited to inguinal saddle hernia. The key of opera-tion success is attaching importance to operation skills.

Objective To investigate the level of five auto-antibodies including MCV and GPI in the serum of RA patients and assess the application value of five auto-antibodies in RA diagnosis.Methods The five auto-antibodies were detected by ELISA in serum samples of 150 patients with RA and 40 healthy controls,32 patients of SLE,30 patients of OA,20 patients of AS,20 patients of SS,20 patients of CTD.Results The positive rates of these five auto-antibodies in RA patients were significantly higher than in other group(X2=88.5,76.0,279.2,88.2,94.8,P＜0.05).Except anti-AKA,there was no the differences in the level of other antibodies among groups(X2=21.9,9.4,20.2,43.2,41.6,P＞0.05).Anti-MCV and anti-GPI has the highest sensitivity(78.0% and 83.3%),while anti-CCP has the highest specificity(97.1%)and anti-AKA has good specificity(96.1%)and lowest sensitivity(49.4%).When two antibodies were detected together,the sensitivity and specificity of MCV/CCP were highest(92.7% and 96.9%).When RF/GPI/CCP were detected together,the sensitivity and specificity were 90.7% and 96.9%,respectively.When RF/MCv/CCP were detected together,the sensitivity and specificity were 94.0% and 96.9%.Conclusions Anti-MCV and anti-GPI has the hishest sensitivity in laboratory diagnosis of RA,while anti-CCP has the highest specificity and anti-AKA have good specificity and lowest sensitivity.The combination detection can decrease the amount of missed diagnosis caused by single test. The combination detection of RF/GPI/CCP and RF/MCV/CCP will improve sensitivity and specificity for diagnosis to the RA patients.

by lfow cytometry. According to CAG morphology, the plaques included 3 types as Type I, Type II and TypeⅢ. The patients also received coronary CT angiography (CTA), upon CTA value, the plaques were classiifed by soft plaque, ifbrous plaque and calciifed plaque. Expressions of platelet surface EMMPRIN and GPVI among different groups were compared.
Results:①Compared with Control group, ACS and SAP groups had increased expressions of EMMPRIN (5.82 ± 0.81 and 3.45 ± 0.48) vs (1.35 ± 0.15) and GPVI (16.22 ± 5.27 and 8.20 ± 2.87) vs (4.14 ±1.17); the expressions in ACS group were higher than those in SAP group, allP<0.05.②In ACS group, compared with Type I plaque, the patients with Type II and TypeⅢ plaques had elevated levels of EMMPRIN (6.35 ± 1.05 and 4.09 ± 0.67) vs (2.45 ± 0.27) and GPVI (19.50 ± 4.55 and 10.81 ± 2.33) vs (5.89 ± 1.28); the expressions in patients with Type II plaque was higher than those with Type III plaque, allP<0.05.③In ACS group, compared with calciifed plaque, the patients with soft and ifbrous plaques had the higher levels of EMMPRIN (6.18 ± 1.01 and 3.87 ± 0.56) vs (2.43 ± 0.25) and GPVI (19.14 ± 4.27 and 11.08 ± 1.94) vs (5.96 ± 0.99); the expressions in patients with soft plaque was higher than those with ifbrous plaque, allP<0.05.④In CAD patients, EMMPRIN expression was negatively related to plaque type (95% CI: -0.359 to -0.206, β: -0.211) and positively related to clinical type (95% CI: 0.893-1.034, β: 0.893); GPVI expression was negatively related to plaque type (95% CI: -1.222 to -0.586, β: -0.181) and positively related to clinical type (95% CI: 3.576-4.164, β: 0.960).
Conclusion: Expression levels of platelet surface EMMPRIN and GPVI were closely related to the stability of coronary plaque, both of them were the risk factors for severe coronary lesions. EMMPRIN and GPVI may have certain predictive value for early diagnosis of arteriosclerosis in ACS patients.

Objective To investigate whether cyclooxygenase-2(COX-2)expression are related to the clinicopathological features,and to microvessel density(MVD).Methods COX-2 expressions were detected in 45colorectal carcinoma samples by immunostaining.MVD was evaluated with the specific antibody CD105.Quantitationof COX-2 mRNA was performed by RT-PCR in 45 specimens of colorectal carcinoma.Results The expressions of COX-2 mRNA and protein were significant variation among lymphatic metastasis,high Dukes stages and grade of differation(P＜0.05);Increased value of MVD were significant variation among lymphatic metastasis,high Dukes stage and depth of tumor invasion(P＜0.05).Both the overexpression of COX-2 protein and mRNA was positively correlated with the value of MVD(P＜0.05).Conclusion COX-2 plays an important role in the development of colorectal carcinoma.COX-2 may up-regulate the value of MVD,which induces angiogenesis and accordingly contributes to metastasis.

Objective:To investigate the clinical features, influencing factors and outcome of cholestasis in preterm infants.Methods:The clinical data of premature infants(case group)admitted to the Neonatal Department of Shengjing Hospital of China Medical University and diagnosed with cholestasis from January 2016 to December 2018 were analyzed retrospectively.Non-cholestatic preterm infants who were hospitalized at the same time, with the same number, gestational age and birth weight were selected as the control group.The clinical data of the two groups were compared to understand the clinical features and risk factors of premature infant cholestasis, and to explore its influence on the development of premature infants.Results:The total cases were 112(the ratio of male to female is 2.02). The gestational age ranged from 25 to 36 weeks, the median gestational age(interquartile interval)was 30(28, 31)weeks, and the gestational age <32 weeks accounted for 78%.The birth weight was 440 to 3 840 g, the median birth weight(interquartile interval)was 1 142(979, 1 300)g, and the birth weight <1 500 g accounted for 83%.The duration of parenteral nutrition, hospital stay, beginning enteral feeding, and reaching total enteral feeding in the case group were 37 d, 62 d, 5 d, and 37 d, respectively, while those in the control group were 23 d, 45 d, 4 d, and 22 d, respectively, with statistical significance( P<0.05). The incidence of necrotizing enterocolitis, patent ductus arteriosus and extrauterine growth retardation in the case group was 16.1%, 15.2% and 91.2%, respectively, while the incidence of corresponding diseases in the control group was 4.5%, 6.1% and 83%, with statistical significance between the two groups( P<0.05). The rate of weight gain after cholestasis was 8 g/(kg·d)in the case group and 13 g/(kg·d)in the control group, and the difference between the two groups was statistically significant( P<0.05). Logistic regression analysis showed that prolonged parenteral nutrition was an independent risk factor for cholestasis in preterm infants. Conclusion:Cholestasis in preterm infants is more common in ultrafast preterm infants and early preterm infants or in infants with extremly low or very low birth weight.The incidence of was increased with cholestasis, and the length of hospital stay was prolonged.Prolonged parenteral nutrition is an independent risk factor for cholestasis in preterm infants.

OBJECTIVE:To study in vivo tissue distribution of genistein-loaded MePEG-PLGA copolymer micelle in mice. METHODS:HPLC method was used to determine the concentrations of genistein in plasma and tissues (heart,liver,spleen, lung, kidney) of mice. The determination was performed on Dikma Diamonsil C18 colunm with mobile phase consisted of methanol-water (60:40,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 260 nm,and the column temperature was 35 ℃. The sample size was 10 μL. 48 mice were divided into emulsion group and micelle group,with 24 mice in each group. They were given 40 mg/kg genistein emulsion or genistein-loaded MePEG-PLGA copolymer micelle via tail vein. The contents of genistein in plasma and tissues (heart,liver,spleen,lung,kidney) were detected 5,30,90 min after medication. AUC0-90 min and mean residence time(MRT)were compared. RESULTS:The content of genistein in micelle group was higher than emulsion group 5,30,90 min after medication,and the content of genistein in liver tissue was lower than emulsion group 5,30 min after medication. Compared with emulsion group,AUC0-90 min and MRT of genistein in plasma of mice in micelle group were increased significantly(P<0.01);AUC0-90 min and MRT of genistein in liver tissue were decreased significantly(P<0.05). AUC0-90 min of genistein in renal tissue was increased significantly (P<0.05),without statistical significance of other differences (P>0.05). CONCLUSIONS:genistein-loaded MePEG-PLGA copolymer micelle can increase the distribution of genistein in plasma,reduce the distribution of it in liver tissue so as to improve therapeutic efficacy and reduce drug hepatotoxicity.