Objective To develop a new method of preparing anti-Rh(D) reagent serum in order to help overcome the shortage of Rh(D) negative red blood cells(RBCs). Methods Anti-Rh(D) containing type A and B sera were mixed. Through dilution and neutralization, the titer of complete antibodies decreased to 2. Anti-Rh(D) saturated Rh(D)positive RBCs were prepared by adding anti-Rh(D) containing serum to Rh(D) positive RBCs so that all the Rh factors on the cells were saturated. The anti-Rh(D) saturated type A and type B RBCs were added to the mixed plasma to absorb the remaining anti-A and anti-B. Results The acquired regent sera had a high anti-Rh(D) titer with a high specificity. Conclusion Anti-Rh(D) saturated Rh(D) positive RBCs absorption after neutralization can help with the production of human origin anti-Rh(D) reagent serum and save a large amount of Rh(D) negative evythvocytes.

Objective To investigate the clinical effect of anterior internal fixation plus vacuum sealing drainage in the treatment of ulnar and radial fractures of Gustilo type Ⅲ.Methods Twenty-eight patients with open ulnar and radial fracture of Gustilo type Ⅲ were managed from April 2007 to March 2014,and were divided into four groups(n =4).Group A were managed with external fixator and conventional changing dressings.Group B were managed with internal fixation and vacuum sealing drainage.Group C were managed with external fixator and vacuum sealing drainage.Group D were managed with internal fixation and conventional changing dressings.Result Twenty-eight cases were adopted telephone follow-up for 6 to 27 months.The soft tissue recovery time of each group respectively was (20.5 ± 2.37) days,(14.7 ±2.16) days,(15.6 ±2.17) days and(19.7 ±2.18) days.The hospital stay of each group respectively was (9.7 ± 2.54) weeks,(4.7 ± 1.46) weeks,(5.2 ± 2.34) weeks and 8.6 ± 2.16) weeks.The fracture healing time of each group respectively was (19.6 ± 2.74) weeks,(13.1±1.84) weeks,(18.1 ±2.54) weeks and (14.7 ± 1.74) weeks.There was significant difference of these data between the two groups(P ＜ 0.05).Conclusions Anterior internal fixation plus vacuum sealing drainage is a better way to treat ulnar and radial fractures of Gustilo type Ⅲ.The technique has short period,less complications,less painful and less expense.

OBJECTIVE: To investigate the influences of staphylococcus aureus in planktonic and biofilm forms on the expression of lysozyme, SLPI and gp340 in the human sinonasal explant model. METHOD: Mucosa samples from ethmoid sinus were collected from ten patients of cerebrospinal fluid leak and were cultured with and without S. aureus biofilms and planktonic cells. After the infection, the explant model was confirmed by CLSM, and the secretion of lysozyme, SLPI and gp340 was detected by enzyme-linked immunosorbent assay (ELISA) at 8, 16, and 24 h after S. aureus challenge. Expressions of lysozyme, SLPI and gp340 in mRNA and protein levels after 24 h S. aureus challenge were detected using RT-PCR, immunohistochemistry and Western bolt assay respectively. RESULT: The secretion of lysozyme, SLPI and gp340 in the explant model was observed with a trend to increase in a time-dependent manner. At 8 and 16 h after S. aureus challenge, the secretion of lysozyme, SLPI and gp340 in biofilms group was significantly higher than these in planktonic cells group and control group (P<0. 05). S. aureus biofilms enhanced the mRNA expressions of lysozyme, SLPI and gp340 significantly compared with planktonic cells and controls, and the mRNA expressions in the explant model challenged by planktonic cells were significantly higher than controls (P < 0.05). Although the Western bolt assay showed no differences between the lysozyme expression in the planktonic cells group and control group (P > 0.05), the biofilms enhanced the expressions of lysozyme, SLPI and gp340 significantly compared with planktonic cells and controls (P < 0.05). CONCLUSION S. aureus biofilm induced the expressions of lysozyme, SLPI and gp340 to a higher level than planktonic cells, indicating that S. aureus biofilm was an influencing factor on the innate immune system.
Biofilms ; Enzyme-Linked Immunosorbent Assay ; Ethmoid Sinus ; metabolism ; microbiology ; Humans ; Immunity, Innate ; Muramidase ; metabolism ; RNA, Messenger ; metabolism ; Receptors, Cell Surface ; metabolism ; Secretory Leukocyte Peptidase Inhibitor ; metabolism ; Staphylococcal Infections ; metabolism ; Tissue Culture Techniques

To identify the gene differentially expressed in female Anopheles anthropophagus and to analyze its gene sequence, this gene amplified by PCR was identified by real-time PCR and its cDNA was then amplified with rapid amplification of cDNA ends (RACE) technology. It was found that the expression ratio of the female differentially expressed gene in female and male mosquitoes was 267.49 according to the formula F=2~(-⊿⊿CT).The size of mRNA of the gene was 364 bp, and the amino acid sequence deduced from the open reading frame (ORF) was found to be similar to the sequence of tectin protein of Culex quinquefasciatus and proteins of other species. The mRNA sequence of this gene was submitted to NCBI with a accession number of FJ907236. This gene may provide a foundation for further studies on the biological functions of mosquitoes.

Objective To screen and identify effective small interfering RNA (siRNAs) targeting the conserved 5’ untrans-lated region (UTR) of the enterovirus 71 (EV71) genome. Methods Double-stranded siRNAs were designed to target the 5’UTR of the EV71 genome. The cytotoxicity effect of siRNAs on rhabdomyosarcoma (RD) cells was evaluated. The cytopathic effect on EV71-infected RD cells was observed under phase-contrast microscopy and the effective siRNAs were screened out by cell viability assay and real-time TaqMan RT-PCR assay. Results All the siRNAs did not exhibit any cytotoxic effects on the viability and growth of RD cells. Transfection of si-1 and si-2 targeting the 5’ UTR in EV71 genome into RD cells signiifcantly delayed and alleviated the cytopathic effects of EV71 infection, increased cell viability and reduced the EV71 RNA transcripts. And the inhibitory effect of si-1/si-2 on EV71 replication was sequence-speciifc. Conclusions The 2 siRNAs (si-1 and si-2) targeting the conserved 5’UTR of the EV71 genome may have broad spectrum antiviral effects on many epidemic EV71 strains.

OBJECTIVE: To analyze the characteristics of respiration events of sleep disordered breathing in children. METHOD: Thirty-two guide polysomnography was monitored in 203 snoring children. According to AHI and AI, they were divided into five groups and the results were analyzed by polysmith. RESULT: The mean value of REM was higher than that of NREM. The mean value between apnea and hypopnea have significant differences (P<0.01). CONCLUSION 1) Respiration events of children mostly happen in REM. 2) The mean value of hypopnea is higher than that of apnea. 3) Diagnosing obstructive sleep apnea hypopnea syndrome in children as AI > or = 1 need research.
Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Polysomnography ; Respiration ; Sleep Apnea Syndromes ; physiopathology ; Sleep, REM ; Snoring ; physiopathology

OBJECTIVE: To investigate the relationship between blood levels of lead and the function states of cochlear outer hair cells (OHC). METHOD: Two hundred and fifty-six children lived in Pb-Zn mine area and Yiliang country, aged from 6 to 7 years were enrolled in this study. Blood lead (B-Pb) levels were measured. Otoacoustic emission (OAE) tests including transient evoked otoacoustic emissions (TEOAE) and distortion products otoacoustic emission (DPOAE) were also measured in these children. RESULT: The difference in B-Pb levels between the school children who lived in mine area and children lived in country was significant (P < 0.01). There were negative correlations between the B-Pb levels and the signal to noise ratio (SNR) on DPOAE test in children who lived in country and those lived in mine area (P < 0.01). The difference in relative coefficients of B-Pb levels between the children lived in mine and children lived in country were significant (P < 0.01). CONCLUSION The B-Pb levels of children who lived in mine area were higher than that of those lived in country. SNR decreased in plumbism cases. Long-term Pb expose may influence the function of cochlear OHC and higher B-Pb levels may lead to worse function of OHC.
Audiometry ; Auditory Threshold ; Child ; Environmental Exposure ; Female ; Humans ; Lead ; blood ; Male ; Otoacoustic Emissions, Spontaneous

Objective:To explore the effect of the transtheoretical model and stages of change (TTM) led by specialized nurses in the symptom cluster management of radiating muscle group in nasopharyngeal carcinoma.Methods:From January to October 2021, 58 nasopharyngeal carcinoma patients hospitalized in the First Affiliated Hospital of Zhengzhou University were selected as the study subject by convenience sampling. According to the random number table method, patients were divided into a control group and an experimental group, with 29 cases each. The control group implemented the routine nursing, while the experimental group used TTM staged nursing led by specialized nurses on the basis of the control group. We collected data before discharge and six months after discharge, and observed the changes in incisor distance, degree of mouth opening restriction, and swallowing difficulty in both groups of patients.Results:Six months after discharge, the distance between the incisors in the experimental group was greater than that in the control group, and the grading of mouth opening restriction was better than that in the control group, and the swallowing difficulty score of the patients was higher than that in the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The TTM staged nursing led by specialized nurses can improve the symptom cluster management of radiating muscle group and the quality of life of nasopharyngeal cancer patients after radiotherapy, which is worthy of clinical practice.

Objective:To explore the effects of solution-focused group therapy nursing intervention on body image and anxiety and depression in young patients with nasopharyngeal carcinoma.Methods:Using a convenience sampling method, 60 young patients with nasopharyngeal carcinoma hospitalized in the First Affiliated Hospital of Zhengzhou University from December 2020 to December 2021 were selected as the study subjects, and were divided into an observation group and a control group using the random number table method, with 30 cases each. The control group received conventional care, and the patients in the observation group implemented a focal solution-oriented group counselling nursing intervention on the basis of the control group. The scores on the Body Image Scale (BIS) and the Hospital Anxiety and Depression Scale (HADS) were compared between the two groups before and after the intervention.Results:Before the intervention, there were no statistically significant differences between the BIS and HADS scores of the two groups ( P>0.05). After the intervention, the total score of BIS and each dimension in the observation group and the score of HADS were lower than those in the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The nursing intervention of solution-focused group therapy can effectively alleviate the body image dysregulation condition and reduce the level of anxiety and depression in young patients with nasopharyngeal carcinoma.