Objective The difference of the equivalent IOL powers before and after LASIK was calculated by using the Haigis-L formula and Sirius ray-tracing respectively.And study the correlation be-tween the intraocular lens power and the myopic before laser in situ keratomileusis.Methods Ninety-one patients undergoing myopic laser in situ keratomileusis were enrolled, they were divided into 3 groups ac-cording to myopic diopter, group I (-1.00D~-3.00D) 13cases, group II( -3.25D and -6.00D)60 cases, group III(-6.25D~-10.0D)18 cases.The equivalent IOL power before and after LASIK will be calculated using Haigis-L formula and Sirius ray-tracing.The data were analyzed using SPSS20.0.Results 80.2%of the cases calculated using Haigis-L formula were within ±0.50D of the predicted refraction , 94.5%were within ±1.00D, and 100%within ±1.50D.also, within ±0.50D of the predicted refrac-tion 13cases(100%),51cases(85%),9cases(50%) in group I, II, III respectively.74.7% of the eyes calculated using Sirius ray-tracing were within ±0.50D of the predicted refraction , 89.0%were within ± 1.00D, and 100%within ±1.50D.within ±0.50D of the predicted refraction 12cases(92.3%),48cases (80%),8cases(44.4%) in group I, II, III respectively.Conclusions Sirius ray-tracing and Haigis-L formula can calculate IOL power accurately in eyes with prior myopic LASIK, with no need for preoperative data.and there is positive correlation between the intraocular lens power aberration and the myopic diopter before LASIK.

Objective:To summarize the diagnosis features of the prenatal genetic diagnosis of fetal renal cystic disease and to explore the clinical feasibility and significance of prenatal genetic diagnosis of congenital cystic nephrosis.Methods:A total of 25 fetuses with congenital renal cystic disease were examined via invasive prenatal diagnosis in Henan Provincial People's Hospital from June 2017 to September 2019. Amniotic fluid samples were extracted by amniocentesis. Chromosomal microarray analysis (CMA) were performed in 17 cases. In addition to CMA, the other 8 cases were analyzed by G-band karyotype. Whole exome sequencing (WES) was performed in 6 cases which got normal results by CMA and karyotype, and highly suspected as hereditary disease.Results:Of the 25 fetuses assessed, 4 cases (16.0%) pathogenic copy number variation (pCNV) were found, including 2 cases of 17q12 deletion, 1 case of 10p15.1p14 deletion and 1 case of 4q21.28q22.1 deletion(including PKD2 gene). There were 8 cases without chromosome abnormality by karyotype analysis. Six clinical WES analysis found NPHS1 gene c.1440+1 G>A and c.925G > T mutations were related to Finnish type congenital nephrotic syndrome in 1 case, PKD1 gene c.6878C>T mutation was related to autosomal dominant polycystic kidney disease (ADPKD) in 1 case, and there was no definitive mutation in 4 cases. Conclusions:CMA and next generation sequencing are powerful tools for accurate diagnosis, treatment and genetic counseling of fetal congenital renal cystic diseases. For congenital cystic nephropathy, genetic detection is helpful to clarify the etiology, and provide more exactly informations for prognosis evaluation, treatment and family genetic counseling.

Objective:To investigate the prognostic significance of D-dimer level in patients with diffuse large B-cell lymphoma (DLBCL).Methods:The clinical data of 70 newly diagnosed DLBCL patients who were admitted to Tianjin People's Hospital from January 2015 to June 2019 were retrospectively analyzed. The optimal cut-off value of D-dimer for survival was determined according to the receiver operating characteristic (ROC) curve, and the patients were grouped. The differences of coagulation related indexes and clinicopathological features between patients with different D-dimer levels were compared. Kaplan-Meier method was used for univariate analysis of overall survival (OS), and Cox regression model was used for multivariate analysis of OS.Results:According to ROC curve, the best cut-off value of D-dimer for survival was 0.75 mg/L. The proportion of patients with different clinical staging, international prognostic index score, lactate dehydrogenase level had statistically significant differences between the D-dimer ≥0.75 mg/L group (36 cases) and <0.75 mg/L group (34 cases) (all P < 0.05). The prothrombin time of D-dimer ≥ 0.75 mg/L group and < 0.75 mg/L group were (13.5±0.9) s and (13.0±0.8) s, respectively, and the activated partial thromboplastin time were (37±5) s and (34±6) s, respectively,and the differences were statistically significant (all P < 0.05). Univariate analysis showed that the 5-year OS rates of DLBCL patients with Ann Arbor stage Ⅲ-Ⅳ, international prognostic index score > 2, lactate dehydrogenase level > 240 U/L, B symptoms, D-dimer level ≥0.75 mg/L were decreased (all P < 0.05). Multivariate Cox regression analysis showed that D-dimer ≥0.75 mg/L was an independent risk factor for OS of DLBCL patients ( HR=0.368, 95% CI 0.144-0.944, P= 0.038). Conclusion:The level of D-dimer can be used as a clinical indicator to judge the prognosis of DLBCL patients, and the prognosis of patients with high D-dimer level is poor.

Objective:To explore the characteristics of executive function and alterations in monoamine neurotransmitters in methamphetamine-dependent adolescents and to analyse the relationship between executive function and monoamine neurotransmitters.Methods:From January to March 2017, totally 50 female methamphetamine-dependent adolescents and 50 male methamphetamine-dependent adolescents were selected as the experimental group in two compulsory isolation drug rehabilitation centres in Sichuan Province, while normal adolescents (50 males and 50 females) matching the age and gender of the experimental group were recruited as the control group in a school.Executive function was tested by the N-back test, colour word interference test and Hanoita test, and serum levels of dopamine, 5-hydroxytryptamine, epinephrine and norepinephrine were measured using high-performance liquid chromatography with fluorescence detection.Statistical analysis was performed by SPSS 21.0 software.The t-test was used to compare the differences of executive function between the experimental group and control group, and Pearson correlation analysis was used to assess the correlation between executive functions and monoamine neurotransmitters in the experimental group. Results:The differences in the number of correct 0-back responses ((105.38±17.00) vs (114.05±5.29) ) and correct response time ((728.82±110.95) ms vs (652.24±89.88) ms), number of correct 2-back responses ((54.78±23.04) vs ( 74.01±12.01)) and correct response time ((585.74±245.35) ms vs (477.44±181.26) ms), the number of correct responses in the Stroop task ((29.68±7.19) vs (33.60±7.36)) and correct response time ((973.73±228.27) ms vs ( 916.11±98.54) ms), and the number of TOH movement steps ((99.42±32.83) vs (87.70±32.55)) were statistically significant in the experimental group compared to the control group(all P<0.05). In the experimental group, serum dopamine ((5.06±1.55) μg/mL vs (3.18±1.97) μg/mL), 5-hydroxytryptamine ((351.94±119.90) ng/mL vs (149.27±69.24) ng/mL), epinephrine ((555.66±225.55) ng/mL vs (129.20± 81.39) ng/mL), and norepinephrine ((3.63±0.96) ng/mL vs (2.03±0.64) ng/mL) were higher than those in the control group, all with statistically significant differences (all P<0.01). Correlation analysis of executive function with monoamine neurotransmitters showed that serum dopamine level in the experimental group was correlated significantly with the number of correct 0-back, 2-back responses, correct response time, and TOH movement steps ( r=-0.194, 0.170, -0.163, 0.198, 0.196, all P<0.05), 5-hydroxytryptamine level was negatively correlated with the number of correct 0-back, 2-back responses( r=-0.267, -0.375), and was positively correlated with correct response time ( r=0.243, 0.177). Adrenaline content was significantly correlated with the number of correct 0-back and 2-back responses, correct response time, and the number of correct Stroop test responses, correct response time ( r=-0.340, 0.212, -0.415, 0.170, -0.212, 0.178, all P<0.05). Norepinephrine level was correlated significantly with the number of correct 0-back responses, correct response times, correct 2-back responses, correct Stroop test responses, and TOH movement steps ( r=-0.245, 0.266, -0.291, -0.193, 0.226, all P<0.05). Conclusion:The executive function of methamphetamine-dependent adolescents is damaged to a certain extent and the content of monoamine neurotransmitter in serum is increased.There is a correlation between impairment in executive function and serum levels of monoamine neurotransmitters.