Optical molecular imaging call non-invasively, quantitatively, real-time and dynamically monitor the biological processes in vivo, and enhance the understanding of disease and drug activity in the drug development process, which has been effectively used for target identification, compounds screening. pharmacodynamic action, pharmacokinetics research and evaluation of drug effect.

Objective To study the structure and the use of the information sharing platform for long-distance medical information network in integrating long -distance medical information network, HIS, PACS system and other resources, thereby enhancing the quality of long -distance medical consultation. Methods The study was conducted by integrating hospital internal data resources, structuring information platform and standardizing consultation process. Results The information sharing platform was achieved based on long-distance medical information network, thus achieving the greatest degree of information sharing and raising the level of long-distance medical consultation.

BACKGROUND: The target gene cannot be deleted at specific time by using conditional gene knockout system. Most gene knockout animals cannot survive during embryonic development and after birth, which limits the study of gene function.
OBJECTIVE: To review the recent advances about time-specific gene knockout technology.
METHODS: An online search in PubMed was performed from the initial stage of database establishment to August 2014. The key words were gene knockout, inducer and Cre/loxP system. The repeated experiment and unrelated articles were excluded during primary search. A summary was made by analyzing time-specific gene knockout technology.
RESULTS AND CONCLUTION: Most important gene knockout animals could not survive after birth, or die at early embryonic stage due to serious physical defects induced by an important gene deletion. Therefore, the progress in the functionality of a gene at a particular time, the genetic characteristics of an individual organism in a particular developmental period, and the treatment of certain acquired disease at a given time have been limited because of the limitations of research methods. However, the occurrence of conditional gene knockout animals, especial y time-specific knockout animals, provides a research model for the study of these areas. This study reviewed the recent advances, development and application about time-specific gene knockout animals, and discussed the significance and broad prospects of time-specific gene knockout technology of specific genes in the field of scientific research.

Objective:To purify recombinant human interleukin 18(rhIL 18) and perform activity assay of renatured rhIL 18 Methods:The rhIL 18 was purified by molecular sieve chromatography MTT assay and RT PCR method were used in activity assay of rhIL 18 Results:The purity of rhIL 18 was up to 96% The renatured rhIL 18 could enhance the cytotoxicity of NK cells and induce IFN ? gene expression in PBMC Conclusion:The rhIL 18 prepared exerts functional activity and can be applied for further studies

Objective To compare sodium supplement and sodium restriction to the effect of the extinction and prognosis of liver cirrhosis ascites, and research the relationship between live cirrhosis ascites and plasma sodium.Methods 119 liver cirrhosis patients were randomly divided into 2 groups :60 cases of sodium supplement and 59 cases of sodium restriction. 60 cases of sodium supplement was kept low-salt diet and intravenous sodium chloride supplement(3 ～5 g/d) ,59 cases of sodium restriction was only kept low-salt diet. Plasma sodium,plasma chloride,urine sodium, urine sodium chloride were detected before treatment, and 6 days, 10 days after treatment respectively. And the urine quantity,extinction of ascites and prognosis were compared. Results There were significant differences on the increase of plasma sodium,plasma chloride,urine sodium,urine chloride and urine quantity after treatment in two groups. Extinction time of ascites was shorter in group of sodium supplement. The morbidity and fatality rate of hepatic encephalopathy, and hepatorenal syndrome in the group of sodium supplement were lower than that in the group of sodium restriction. Conclusion Sodium supplement should be adapted when using diuretic agent to help the extinction of ascites, and to improve recovery.

Objective To the value of ultrasonic imaging in diagnosis of appendiceal mucinous neoplasms in patients.Methods A retrospective review was performed for the 26 cases undergoing pre-operative ultrasonography and finally diagnosed as appendiceal mucinous neoplasms by pathology.The 26 patients were included in the study,11 elderly patients aged ≥60 years as group A,and 15 younger patients aged ＜ 60 years as group B.The characteristics of the ultrasonic imaging were analyzed.Results Features of ultrasound images leading to diagnosis were summarized as following.The lesion transverse diameter was over 2 cm,with anechoic or hypoechoic area of lesion or onion skin sign inside the lesions.There were distinct borderlines between lesions and surrounding tissues,with no or little blood in the color doppler flow imaging.Conclusions There are same u1trasonography characteristics of appendiceal mucinous neoplasms in two age groups.Improving the knowledge of this disease and grasping the clinical and sonography characteristics are stringent to enhance the accuracy rate of diagnosis.

Objective To analyze the characteristics of hospitalized patients with systemic lupus erythematosus (SLE) in Ningxia Hui Autonomous Region,and to characterize the epidemiology of SLE.Methods The data of 184 patients admitted to Ningxia Medical College Hospital during January 2008 to December 2013 were retrospectively analyzed.The data analysis included the initial symptoms,system involvement and clinical situations.Chi-square test,t test,Mann-Whitney U test and Logistic analysis were used for statistical analysis.Results ① The ratio of male and female was 1∶9.8.The most common clinical symptoms was rash in 72 cases (39％),and followed by joint pain in 61 cases (33％),swelling in 28 cases (15％) and fatigue in 21 cases (11％).② Eighteen percent (33/184) patients were late-onset SLE patients.Compared with regular SLE patients,the interval was longer in late-onset SLE patients between clinical symptoms onset and the time when they saw the doctor.③ Blood and kidney were the most commonly affected organs.④ The highest diagnosis rate occurred in rheumatology department,the highest diagnosis rate occurred in the kidney department for patients with dropsy as starting symptom.Conclusion SLE primarily occurs in childbearing aged women.The reason of misdiagnosis of SLE is mainly due to the incorrect hospital selection for the first visit.Delayed diagnosis will lead to delay in the treatment.