Objective To establish a method for the detection of methylene tetrahydrofolate reductase (MTHFR) 677 C→T mutation by real-time fluorescence PCR.Methods MTHFR 677 C→T mutation was detected in 300 healthy people by the technique of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and real time fluorescence PCR. Sequencing was performed to confirm the accuracy of detection results. Results Three genotypes (CC, CT and TT) were found with two methods in all the 300 people. There were no significant differences in genotype frequency and allele frequency between the two groups (P>0.05).Conclusion Real-time fluorescence PCR is an accurate and rapid method for prompt grouping of MTHFR gene locus.

Objective:To explore the clinical application value ofChinese evaluation method of 10-year morbid risk of ischemic cardiovascular diseasesself developed from China.Methods:Chinese evaluation method of 10-year motbid risk ofischemic cardiovascular diseasesdeveloped bv cardiovascular diseaseinstitute of Chinese academy of medical science,was used to evaluate motbid risk of cardiovascular diseases of 2465 community residents in Chaoyang district of Beijing.Relative analysis was done then.Results:(1)Compared with female group,mean values of systolic blood pressure[(122.84±16.42)mmHg vs.(126.83±16.36)mmHg],diastolic blood pressure[(80.67±9.51) mmHg vs.(84.54±9.89)mmHg]and total cholesterol[(4.86±1.06)mmol/L vs.(5.01±0.97)mmol/L]significantly increased in male group,P＜0.001 all;(2)For the ratio of subjects whose 10-year morbid risk of ischemic cardiovascular diseases≥10%(high risk subjects),was of 3.28% in men and significantly more than that of women(1.59%)P ＜0.05.It was consistent withthe data of absolute risk ofischemic cardiovascular diseasesfrom Chinese 9903 cases survey and follow up 15.1 years by the collaborative research group of the Chinese national 10th five years plan project.Conclusion:Chinese evaluation method of 10-year morbid risk of ischemic cardiovascular diseasescan accurately detect distribution condition of morbid risk among population andit makeforto simple screening of high risk population.

Objective: To explore the clinical application value of “Chinese evaluation method of 10-year morbid risk of ischemic cardiovascular diseases”self developed from China. Methods: “Chinese evaluation method of 10-year morbid risk of ischemic cardiovascular diseases”developed by cardiovascular disease institute of Chinese academy of medical science, was used to evaluate morbid risk of cardiovascular diseases of 2465 community residents in Chaoyang district of Beijing. Relative analysis was done then. Results: (1) Compared with female group, mean values of systolic blood pressure [(122.84±16.42) mmHg vs. (126.83±16.36) mmHg], diastolic blood pressure [(80.67±9.51) mmHg vs. (84.54±9.89) mmHg] and total cholesterol [(4.86±1.06) mmol/L vs. (5.01±0.97) mmol/L] significantly increased in male group, P<0.001 all; (2) For the ratio of subjects whose 10-year morbid risk of ischemic cardiovascular diseases≥10% (high risk subjects), was of 3.28% in men and significantly more than that of women（1.59%） P<0.05. It was consistent with the data of absolute risk of ischemic cardiovascular diseases from Chinese 9903 cases survey and follow up 15.1 years by the collaborative research group of the Chinese national 10th five years plan project. Conclusion: “Chinese evaluation method of 10-year morbid risk of ischemic cardiovascular diseases”can accurately detect distribution condition of morbid risk among population and it make for to simple screening of high risk population.

OBJECTIVE:To detect curative mechanism of bushen huoxue huatan formula on polycystic ovarian syndrome (PCOS)model rats. METHODS:50 rats were randomly divided into normal control group,model control group,positive control group [rosiglitazone,3.0 mg/(kg·d)] and Bushen huoxue huatan formula high-dose,low-dose groups [7.5,5.0 g/(kg·d)],10 in each group. Except for normal control group,the PCOS model was induced by Poresky method in other groups. After modeling, normal control group and model control group were received normal saline intragastrically,medicine groups were received corre-sponding liquids intragastrically,once a day,for 22 d. After administration,the serum levels of testosterone (T),anti-mullerian hormone (AMH),insulin-like growth factor Ⅰ(IGF-Ⅰ),tumor necrosis factor-α(TNF-α) and the positive expression of trans-forming growth factor-β1(TGF-β1)in ovarian tissue were determined. RESULTS:Compared with normal control group,T,AMH, IGF-Ⅰ,TNF-αlevels in serum and TGF-β1 positive expression rate in ovarian tissue of rats in model control group were significant-ly increased(P<0.05);compared with model control group,T,AMH,IGF-Ⅰ,TNF-α levels in serum and TGF-β1 positive ex-pression rate of rats in ovarian tissue in other medicine groups were significantly decreased(P<0.05),and the indexes in Bushen huoxue huatan formula different doses groups were superior to positive control group(P<0.05). CONCLUSIONS:Bushen huoxue huatan formula can obviously reduce the T,AMH,IGF-Ⅰ,TNF-α levels in serum and TGF-β1 positive expression rate in ovarian tissue in model rats with PCOS,which may be one of the mechanisms of its treatment for PCOS.

OBJECTIVE: To explore the factors,general pattern and characteristics of adverse drug reactions(ADR) in children.METHODS: A total of 188 pediatric ADR reports collected in our city from Jan.2002 to Sept.2007 were statistically analyzed in respect of children's age and sex,route of administration,ADR severity,category of drugs involved,organs or systems involved and clinical manifestations etc.RESULTS: Of the 188 pediatric ADR cases,95(50.5%) were induced by intravenous route.A total of 112 kinds of drugs were involved in the ADR,with anti-infective agents showing the highest proportion.Digestive system,neural system,urinary system and skin etc were involved in the ADR.CONCLUSION: Clinic should attach great importance to the monitoring and reporting of pediatric ADR to reduce or avoid the occurrence of ADR.

Objective:To study the neuroprotective effect of nobiletin on the symptoms of postoperative cognitive impairment (POCD) induced by sevoflurane inhalation.Methods:Twenty-four aged SD rats (12 female mice and 12 male mice) were divided into three groups randomly: control group ( n=8), surgery group ( n=8) and surgery + nobiletin group ( n=8), with 4 females and 4 males in each group.The rats in surgery group and surgery+ nobiletin group were given normal saline(0.1 ml/10 g, once a day) and nobiletin(100 mg/kg, once a day) intragastrically for 6 weeks.Then the rats were anesthetized by sevoflurane and treated by abdominal exploration surgery, and then continued gavage for 1 week.The rats in control group were given normal saline(0.1 ml/10 g, once a day) intragastrically for 7 weeks without anesthesia or surgery.Sevoflurane inhalation anesthesia and abbreviated laparotomy were not done for control group.Morris water maze and open field experiment were used to measure the memory and cognitive ability and the independent exploration ability respectively.The changes of α-band electroencephalogram (EEG) were recorded by multi-channel physiological signal acquisition and processing system.The concentration of S100β, a marker of neurological impairment was detected by ELISA.Western blot was used to detect the expression level of IBA-1 in microglia.SPSS 20.0 software was used to analyze the data. Results:There were no significant differences in Morris water maze, positioning cruise test and open field test among the groups before operation (all P>0.05). The differences were statistically significant among the groups 7 days after operation (all P<0.05). Compared with the control group (the escape latency, path length and cross platform times were ((20.37±1.11)s, (552.37±14.19)cm, (6.75±0.43)times respectively), the escape latency ((40.87±2.03)s) and path length ((1 258.62±19.53)cm) of rats in surgery group were significantly longer (both P<0.01), and the cross platform times ((2.12±0.33)times) significantly reduced ( P<0.01). The differences between surgery + nobiletin group ((22.37±1.11)s, (584.50±10.90)cm, (6.62±0.48)times) and control group were not significant (all P>0.05). The open field experiment showed that the movement distance, the crossed square number, and activity times in surgery group ((1.78±0.55) m, (4.75±0.50), (14.87±0.33) times) decreased significantly compared with those in control group ((3.73±0.07) m, (11.10±0.78), (51.12±0.78) times, all P<0.01). No significant difference was found between surgery + nobiletin group ((3.76±0.07)m, (10.75±0.66), (50.75±0.43)times) and control group(all P>0.05). Before anesthesia, there was no significant difference in the power ratio of α-band among the three groups ( P>0.05), but the differences during anesthesia and operation were significant ( F=72.58, 101.50, P<0.01). During anesthesia and operation, the power ratio of α-band in anesthesia and in surgery group (2.51±0.04, 2.14±0.03) were significantly lower (both P<0.01) than those in control group (3.49±0.03, 3.49±0.03), while there was no obvious changes (both P>0.05) in the surgery + nobiletin group (3.50±0.04, 3.51±0.04). There were significant differences in Bcl-2 protein expression and caspase 3/7 protein activity among the three groups ( F=5.21, 7.84, P<0.01). Compared with control group (1.00±0.02, 1 557.46±3.63), Bcl-2 of rats in the surgery group(0.40±0.05) were significantly lower and Caspase3/7 expression of surgery group (3 689.58±10.46) was significantly higher (both P<0.01), while the rats in the surgery + nobiletin group had no significant difference in both Bcl-2 level (1.03±0.06) and caspase 3/7 activities (1 805.28±6.17, both P>0.05). The difference of S100 β protein expression was significant among the three groups ( F=490.80, P<0.01). Compared with the control group ((0.18±0.01)μg/L), the concentration of S100β protein in the surgery group ((2.13±0.02)μg/L) decreased ( P<0.01), while there was no significant difference in the surgery + nobiletin group ((0.16±0.01) μg/L, P>0.05). The expression levels of IBA-1 protein ( F=10.83) and TNF-α, IL-1, IL-1β and IL-6 ( F=996.20, 221.40, 73.02, 174.13) were significantly different among the three groups (all P<0.01). The expression level of the neuroglial marker IBA-1 in the surgery group(1.36±0.02) was significantly higher than that in the control group (1.00±0.01, P<0.01), while the surgery + nobiletin group (1.03±0.01) had no significant different compared with control group ( P>0.05). The levels of inflammatory factors, including TNF-α, IL-1, IL-1β and IL-6, in the brain of rats treated with nobiletin ((49.06±3.63)pg/mg, (2.09±0.43)pg/mg, (16.27±0.80)pg/mg, (2.11±0.19)pg/mg) were significantly lower than those in the surgery group((145.10±6.46)pg/mg, (5.67±0.43)pg/mg, (27.88±3.43)pg/mg, (4.74±0.32)pg/mg, all P<0.01). Conclusion:Nobiletin can obviously alleviate POCD symptoms caused by sevoflurane inhalation anesthesia.

Objective : To investigate the status of occupational stress, burnout, and coping styles, and to explore the moderating effects of coping styles on the relationship between occupational stress and burnout among medical staff from one tertiary first-class hospital in Hubei Province, in order to provide the basis for mental health promotion of medical staff. Methods: From June to October in 2020, using stratified cluster sampling, doctors, nurses, and medical or pharmaceutical technicians on the ratio of 2∶2∶1 were selected from the tertiary first-class hospital. Chinese version of Burnout Questionnaire, Core Occupational Stress Scale (COSS) and Trait Coping Style Questionnaire were used to investigate the levels of burnout, occupational stress and coping styles. A linear stratified regression model was used to analyze the moderating effect of coping styles on the relationship between occupational stress and burnout. Results: Among 735 questionnaires issued, 679 valid ones were collected, with the response rate of 92.38%. The age of the respondents was ( 34.29±8.20 ) years old. There were 165 ( 24.30% ) males and 514 ( 75.70% ) females, including 241 ( 35.49% ) doctors, 358 ( 52.72% ) nurses and 80 (11.78%) medical or pharmaceutical technicians. The burnout score was 2.29±1.11 , and the positive rate of burnout was 44.33%; the COSS score was 47.32±8.31; the positive and negative coping style scores were 30.64±6.06 and 26.81±6.26, respectively. The linear stratified regression analysis suggested that the interactions between social support ( β=0.590, P<0.05 ), organization and reward ( β=0.523, P<0.05 ), and positive coping style were positively associated with burnout, and the interaction between organization and reward (β=-0.666, P<0.05) and negative coping style was negatively associated with burnout. Conclusions About 44.33% of medical staff investigated have burnout. Coping styles have a moderating effect on the relationship between occupational stress and burnout. Positive coping style helps to perceive social support and organizational support, to alleviate the adverse effects of occupational stress, and to reduce the risk of burnout.

Based on the limitation of the segmental activityand signs of local tenderness,location diagnosis andtreatment for cervical pain and referred pain was per-formed with elevating—rotation reduction.Clinically, attention should be given to the combination of X—rayfilm and signs and symptom.The total effective ratefor 3687 eases thus treated was rather high.

Objective To observe the effect of Huoxue Jiedu Recipe(Herbal recipe for activating blood and removing toxins)on proliferation and cell cycle status of keratinocytes cultured in vitro in psoriasis.Methods Using the serum pharmacologic technique,the clean SD rats were adopted to make the Huoxue Jiedu Recipe medicated serum(high,moderate,low dose),Dexamethasone medicated serum,and blank serum,which were added into the keratinocytes culture system respectively.After incubated for 48 hours,the cell proliferation of keratinocytes was measured by MTT assay,and the cell cycle distribution estimated with flow cytometer(FCM).Results The different density of Huoxue Jiedu Recipe could all inhibit significantly the generation of COLO-16 keratinocyte line and change the distribution proportion of each phase in the cell division cycle.Conclusion Huoxue Jiedu Recipe treats psoriasis through inhibiting the proliferation of keratinocytes.

Total 1 500 subjects aged over 60 years were selected from 10 community health service centers with cluster sampling method.A questionnaire survey and physical examination were performed in all subjects.Sixty five patients with chronic heart failure were detected.The questionnaire showed that 77.6％ (38/49)patients were diagnosed in tertiary hospitals,and 12.2％ (6/49) were diagnosed in community hospitals.When heart failure occurred,most patients (37.7％,20/53) chose to take a taxi to the tertiary hospital directly,and less patients (17.0％,9/53)contacted community doctors.When a heart attack happened,68.8％ (33/48)of the patients could reach the hospital within 30 min,and 55.3％ (26/47) within 60 min.45.7％ (21/46) elderly patients were hospitalized for heart failure once a year;31.1％ (14/45) were in hospital for less than 10 d each time.46.0％ (23/50) heart failure patients had received health education and 40.0％ (16/40) had taken drugs under supervision;the majority health educators were community doctors and majority supervisors were family members.The results suggest that the confirmed diagnosis and treatment of heart failure patients still rely on tertiary hospitals,community health institutions play an important role in health education and management of heart failure patients outside hospitals.But the utilization of community health services is still not enough in community health service centers.