Capsules ; Drugs, Chinese Herbal ; Hepatitis B, Chronic ; Humans ; Liver Cirrhosis

Objective To validate the analytical performance of four LP(a)reagents with Immunoturbidimetry method used on the automatic biochemistry analyzer for preliminary clinical application.Methods The performance of four LP(a)reagents (labeled as A,B,C,D)with method from RANDOX,Zhejiang Kuake Co.,Beijing Leadman Co.and Beijing Jiuqiang Co.on Olympus AU5800 automatic biochemistry analyzer were assessed.The precision,linearity range,accuracy,disturbance (vita-min C,bilirubin,hemoglobin,TG)were assessed.Results The within-run CVs of the four reagents (A,B,C and D)were 0.64%~1.18%,3.59%~4.75%,1.33%~3.05% and 1.43%~2.01% respectively.The between-run CVs in A,B,C and D were 1.04%~1.7%,3.81%~4.93%,2.16%~4.76% and 2.33%~3.21% respectively,lower than the stated.The lin-earity range was 82~923 mg/L (r2 =0.997),130~935 mg/L (r 2 =0.996 4),120~1025 mg/L (r 2 =0.992 1)and 117~943 mg/L (r2 =0.999 5)in the four reagents,which demonstrated a sound linear correlation.For interference tests,no re-markable interferences (<±10%)of reagent A and reagent D were detected when Vitamin C≤10 mg/dl,hemoglobin≤200 mg/dl,bilirubin≤40 mg/dl and TG≤500 mg/dl.Interference of reagent B was found when VC≥5 mg/dl,TG≥250 mg/dl and when TG≥250 mg/dl reagent C was interfered significantly.The four LP(a)reagents were used to detect the lipid con-trol,and the deviations of the target value were - 8.07%,1.34%,- 8.05% and 7.38% respectively.Conclusion When used in automatic biochemical analyzer,the four LP(a)reagents showed high precision.The four reagents are all able to meet clinical test requirements,nevertheless,anti-interference capability were different.

Objective To verify the performance of three kinds of ischemia‐modified albumin(IMA) reagents .Methods The performance of three IMA reagents(labeled as reagent A ,B ,C) using colorimetric method from Shanghai Aikang Biotechnology Co .,Ltd .,Zhejiang Kuake Bioscience Technology Co .ltd .and Beijing Jiuqiang Biotechnology Co .,Ltd .were assessed by using O‐lympus AU5800 automatic biochemistry analyzer .According to the National Committee for Clinical Laboratory(NCCLS) EP6‐A , EP15‐A and EP7‐A documents and WS/T 420‐2013 verificationof analytical performance of quantitative kits by clinical labo‐ratory ,the precision ,linearity range ,accuracy and anti‐interference capability were assessed .Results The within‐run coefficient of variations(CVs) of reagent A ,B and C were 0 .59% -0 .82% ,0 .27% -0 .54% and 0 .62% -0 .69% respectively .The between‐run CVs of reagent A ,B and C were 0 .98% -1 .74% ,0 .99% -1 .01% and 0 .71% -0 .78% ,respectively ,which were lower than decla‐rations of these reagent kits .The linearity range of reagent A ,B and C were 11 -142 U/mL(r2 = 0 .993) ,10 -120 U/mL(r2 =0 .996) ,14-123 U/mL(r2 =0 .992) respectively ,which showed good linearities .About interference tests ,no remarkable interfer‐ences(all Bias were less than ± 10% ) of reagent A ,B and C were detected when Vitamin C≤10 mg/dL ,hemoglobin≤200 mg/dL , bilirubin≤40 mg/dL and triglyceride≤500 mg/dL .Conclusion The three IMA reagents show high precision ,which could meet clinical requirements ,nevertheless ,differences of anti‐interference capabilities are observed in these three reagents .

ObjectiveTo evaluate the therapeutic effect of endoscopic full-thickness resection (EFTR) for gastric stromal tumors.MethodsA total of 33 patients with gastric stromal tumor orgination from deep muscularis propria layer received EFTR from January 2010 to July 2011.The effectiveness and safety of EFTR were compared with those of other 34 patients with gastric stromal tumor origination from muscularis propria layer who underwent endoscopic submucosal dissection (ESD).ResultsExcept in 2 patients with lesions larger than 3.0 × 3.0 cm,EFTR was successful in others 31 patients,who recovered well and had no recurrence during the follow-up within 12 months.There were no significant differences in resection rate,incidence of complications,body temperature,white blood cell counts or recovery time between 2 procedures (P ＞ 0.05 ).However,the number of clips used in EFTR ( 7.0 ± 3.5 vs.4.9 ± 3.1,P =0.013 ) and postoperative fasting days (3.4 ± 1.5 vs.2.0 ± 1.0,P =0.001 ) were significantly higher than those of ESD procedures.ConclusionEFTR is effective and safe for gastric stromal tumors with no higher risk than ESD,but it is more complex technically.EFTR can be used as an expanding method of ESD in endoscopic treatment of gastric stromal tumors.

Objective To discuss MRI features of bone marrow edema in avascular necrosis of femoral head(ANFH),and to evaluatethe relationship between bone marrow edema and the stage of ANFH.Methods MRI findings of 99 hips in 73 patients with ANFH wereretrospectively analyzed.Using SE T_1WI,T_2WI and STIR scaning,both coronal and axid were performed in all patients.The bone marrow edema in ANFH was graded into 0~3,which correlated with the stage of ANFH was concerned.Results Bone marrow edema of the femur was best demonstrated on T_2WI and STIR coronal images.Grade 1~3 bone marrow edema were seen in 61% diseased femoral heads.Bone marrow edema of the femur was increasing with progressing of the disease.Bone marrow edema was not commonly seen and localized in stageⅠof ANFH,but it was more commonly seen and extensive in stage Ⅱ,Ⅲ of ANFH.Conclusion Bone marrow edema is one sign that isaccompanied with ANFH.The probability and extent of bone marrow edema correlated well with the stage of ANFH.MRI is the mostsensitive and non-invasive means for demonstrating bone marrow edema.

Objective To investigate the relation of pelvic three-dimensional conformal radiotherapy(3D-CRT) target with rectal dose distribution and irradiation volume in cervical cancer.Methods In 45 patients with cervical cancer(Ⅱa-Ⅲb) treated in our department from September to December 2006,we compared rectal dose distribution in different treatment plans: conventional radiation,4-field box radiation,4-field oblique radiation and 5-field radiation,and studied the relationship between rectal irradiation volume and dose in the target area.Results Compared with that of the other models,the dose distribution of CTV in 3D-CRT was relatively poor although it could satisfy the demand of treatment;dose distribution of D90,D80 and D50 in the radiated rectum differed significantly(P

The average daily selenium intake of staff of the Institute of Health in Beijng was surveyed to be 66.4?g and that of children in nursery and kindergarten was 34.7?g, which could meet the Recommended Dietary Allowance as suggested. Cereals were the major source of dietary selenium which accounted for 63.6% of total dietary intake, and animal and plant foods provided about 25.9% and 10.5% respectively. Both cereals and animal foods were the major sources of selenium in children diet, which accounted for 48.9% and 44.3% respectively, and intake of selenium from other sources was negligible.The selenium concentrations in whole blood and hair of the population were 0.146ppm and 0.578ppm respectively, and both correlated well with the daily selenium intake.The daily intake of mercury, arsenic, and cadmium for population in Beijing were estimated to be 3.4, 52.7, and 42.7*g respectively, which were within the normal range of intake and would not significantly interfere with the bioavailability of selenium ingested. It was suggested thal these levels of selenium daily intake surveyed would be adequate for human consumption and could be helpful as a reference for the establishment of dietary allowance.

The content of selenium and several antagonistic metals such as mercury, arsenic, and cadmium in foods from Beijing market has been determined. Results showed that marine products, Viscera (particularly kidney), eggs, and mushroom were good dietary sources of selenium. The selenium contents of imported cereals and pulse are much higher than those grown in China as far as the sample analysed. Most vegetables and fruits contained selenium below a level of 0.010ppm with exception of garlic and mushroom.The selenium content of human milk was higher than other kinds of milk, milk products and milk-substitutes. Selenium content of Beijing foods was far higher than those in affected area of Keshan disease. The mercury, arsenic, and cadmium content of foods was below the National Allowance Standard except a few marine products.The selenium content of natural plant foods was positively correlated with its protein content (r=0.844, p

Results obtained from previous studies shoved that at low level of dietary selenium(0.03 ppm) weanling rats fed with diets of low protein level usually possessed higher selenium concentration and GSHPx activity in their blood and tissues. The results were reproduced in chickens in this observation. Furthermore, even the original selenium level could not be maintained in blood and tissues of chickens fed with low protein diet (12.4%) because of faster growth rate and the lower selenium content (0.01 ppm) of the diet used in this study.Of the 27 chickens consuming 18.2% protein diet in the 14 days expe- riment, 23 suffered from exudative diathesis and 3 died without any sign of exudative diathesis, while in the 12.4% protein group only one of the 26 chickens suffered from exudative diathesis during the 14 days experiment and 6 of the 12 chickens in this group observed for another two weeks showed exudative diathesis. In the 18.2% protein group the first chicken with signs of exudative diathesis appeared on the 9th day while it appeared on the 14th day in the 12.4% protein group.How to improve the selenium nutritional status of the residents in Ke-shan Disease areas was discussed.

Objective:To evaluate the efficacy and safety of oral prednisone acetate and oral prednisone acetate combined with local injection of triamcinolone acetonide for the prevention of esophageal stricture after endoscopic submucosal dissection (ESD) for early esophageal cancer and precancerous lesions.Methods:Data of 52 patients with early esophageal cancer or precancerous lesions hospitalized from December 2014 to February 2019 in Zhongda Hospital of Southeast University were analyzed retrospectively. They were divided into the control group (group A, n=20), oral hormone group (group B, n=17) and oral medication combined with local injection group (group C, n=15). The rates of stenosis and refractory stenosis, endoscopic dilatation times after ESD, time interval of first dilation after ESD and adverse events related to the operation and administration of glucocorticoids were compared among the three groups. Results:The differences in stenosis rates [85.0% (17/20) VS 47.1% (8/17) VS 46.7% (7/15), P<0.01], rates of refractory stenosis [75.0% (15/20) VS 23.5% (4/17) VS 20.0% (3/15), P<0.01], and endoscopic dilatation times [3.50 (2.25, 6.00) VS 0.00 (0.00, 2.50) VS 0.00 (0.00, 2.00), P<0.01] were statistically significant among the three groups. In pairwise comparison, the above indicators in group B and group C were significantly lower or less than those in group A ( P<0.05), but there were no statistical differences between group B and group C ( P>0.05). The time intervals of the first dilation after ESD were significantly different among the three groups (27.7±9.4 d VS 110.1±46.0 d VS 147.4±9.4 d, P<0.01). In pairwise comparison, the first dilation intervals in group B and group C were longer than that in group A ( P<0.01), and this indicator in group C was longer than that in group B ( P<0.01). Two patients in the control group developed perforation after ESD but they were treated actively and recovered finally. No severe ESD, steroid, or perforation related adverse events occurred in any other patient of the three groups. Conclusion:Both oral prednisone and oral prednisone combined with local injection of triamcinolone acetonide after ESD can effectively and safely prevent esophageal stricture after ESD. Oral administration combined with local injection of corticosteroids can prolong the time interval of the first dilation after ESD, which is beneficial to the psychological recovery and the improvement of patients’ life quality after the operation.