Objective To evaluate the effectiveness of concept maps in developing critical thinking ability in nursing baccalaureates. Methods Divided 60 nursing students into the experimental group and the control group randomly, there were 30 cases in each group. Traditional nursing care plans was used in the control group, while concept maps was used in the experimental group when analysis typical case. Evaluated the critical thinking ability between the two groups by CTDI-CV at the beginning and the end of term. Results The difference of CTDI-CV scores in the experimental group at the beginning and the end of term was significant, while the difference in the control group was not. Conclusions Concept maps can effective improve nursing students' ability of critical thinking.

Objective Based on the educational concept of competency based education,this paper analyzed the present situation and problems of nursing occupational protection education and occupational protection in china,and explored the necessity of setting up the nursing occupational toxicology and nursing occupational protection in the nursing colleges.Methods The whole tentative plan was presented through the analysis of the course description,teaching method,curriculum content and objectives,curriculum evaluation of nursing occupational toxicology and nursing occupational protection in colleges.Results Students will be able to really aware of the health dangers of various toxic and harmful substances,to set up the correct ideas of occupational protection,to actively taken effective protective measures,to avoid personal injury accidents by studying in this program.Conclusions A course of occupational toxicology study and nursing occupational protection in nursing colleges is suggested to enhance the students' ability of occupational protection.It is imperative for nursing students to complete the degree program.

BACKGROUND:Side population(SP)cells,with varied contents,are widely distributed in adult tissues,embryos,and certain tumor cells.Haematological tumor is one of the main pathological conditions,which endangers human life.Thus,it is important to recognize SP cells in haematological tumor cell lines.OBJECTIVE:To identify whether hematologic tumor cell lines contain SP cells,and to explore a stable detection and separation methods.METHODS:Cells with the characteristics of stem cells being capable of fluorescent dye Hoechst33342 were isolated by flow cytometry;whether there were SP cells in logarithmic growth period of NB4,Raji,K562/ADM and K562 or not were detected.After sorting K562 subpopulations,the purity of sorted cells was detected.RESULTS AND CONCLUSION:After Hoechst33342 staining,flow cytometry results showed that the SP cells appeared in the haematological tumor NB4,Raji,K562/ADM and K562 cell lines,which accounted for 0.8%,2.7%,1.3% and 2.7%,respectively.These cells could be blocked by Verapamil.The purity was greater after a second detection of SP and Non SP cells in K562 cells.

95%,WT= 1 271 6 .Lymphoproliferation reaction of the splenocytes derived from BALB/C mice was inhibited by stimulating with the antisense peptide pre treatment of allogeneic splenic cells and cardiac cells of C57 mice.The inhibition rates up to 38 4% and 36 6%.Pre treatment of allogeneic cardiac grafts resulted in prolonging the survival of cardiac transplantation 5 4 days more than the control group(n=6,P

Objective: To observe the preventive effect of taurine on alcoholic liver damage in rat.Methods: The model was induced by filling stomach with alcohol, and the treatment groups were given two different concentrations of taurine, and the control group was given 0.9%NS, then we measured the blood triglycerides (TG), and the pathological changes of liver quantitatively.Results: Two different le-vels of taurine can significantly decrease the level of TG and ameliorate the liver damage of rats (P0.05), and the 5% taurine group can also decrease the mean arterial pressure (P

Objective To explore the diagnosis value of ultrasound-guided COre needle biopsy(CNB)and frozen sectionin breast cancer.Methods There were 41 cases of breast tumor which were suspicious with ultrasonography,and using Bard automatic biopsy device was applied for percutaneous puncture by ultrasound-guided in breast neoplasms.Tissue samples were harvested for frozen soetion pathologic examination.compared with 42 clinical cases of the turnour which suspicious with ultrasonography were excised in operation room(B group)during the same time.Results The outcome of frozen sections were finished in 30～40 miutes.Thirty-nine cases were diagnosed to be malignant,36 cases were infiltrated ductal carcino and 3cases were infiltrated lobular carcinoma,two cases were severely non-type hyperplasia the sensitivity of sonographically guided CNB for the diagnosis of breast cancer was 95.1%.one operation was finished in 60 to 120 minutes,the average is 68.19±12.41 minutes.In group B,sensitivity of diagnosis of breast cancer was 97.6%(P＜0.05).One operation was finished in 120 to 200 minutes,the average was127.88±11.50 minutes(P＜0.05).Conclusion It can achieved higHy diagnostic rate,and have benefit of setting down the operations program and the operating time was curtailed when breast cancer was utrasound-guided by CNB and examinede with frozen section.

Objectives:The time of Carotid intervention for recently symptomatic,severe carotid stenosis which cause a transient ischemic attack or minor stroke is still a controversial issue.Early studies showed that carotid endarterectomy (CEA) caused a high risk if performed within days follow an acute ischemic stroke.However,The National Stroke Strategy posted by UK Department of Health advocated that this situation should be regarded as an emergency procedure,and carotid intervention should ideally be performed within 48 hours.We designed this study to discuss the effect of urgent CEA on operative risk and benefit.Methods: we analyze 12 urgent CEA with primary closure performed during 1996 to 1998.All 12 patients were underwent CEA within 2 weeks,and 2 of them within 2 days.Operative risks and overall benefit from surgery were discussed in relation to the time from the last symptomatic event to CEA.Results: 2 urgent CEA performed in 2 days are recovery uneventful.1 of 12 patients,who underwent urgent CEA within 2 weeks,occurred restenosis after 3 months follow up.No 30-day perioperative recurrent TIA,stroke and death.Conclusions:CEA performed within 2 weeks is feasible and reliable procedure in preventing recurrent TIA and stroke after presenting manifestations.The future aim is to perform CEA within 48 hours after TIA or stroke symptoms.

An investigation of inappropriate feeding behavior and its impact was conducted in patents of 875 infants and toddlers aged 1-24 months from March 2004 to February 2008.The survey revealed that inappropriate feeding behavior included feeding sugar solution or sweet fruits daily in 72.1％ parents (631/875) ; adding yolk as the first supplementary food in 51.3％ (394/768) ; not allowing handgrabbing food or coarse food for infants over 7 months in 50.4％ (270/536) and 44.0％ (236/536) ; no meat eating for infants over 12 months in 36.0％ (54/150).And also revealed that 34.0％ (270/795) and 24.8％ (184/743) parents mixed rice flour or yolk with formula milk,respectively; 17.6％ (48/273) infant over 10 months only had cereal; insufficient or excessive water were used for reconstituting milk powder in 11.8％ (87/735) and 9.3％ (68/735),respectively.The results of investigation indicate that inappropriate feeding behavior among infants and toddlers are quite common and the reasons are various.

Objective To investigate the changes of hypoxia-inducible factor(HIF)-1α and hexokinase-Ⅱ(HK-Ⅱ)expression in human esophageal squamous cell carcinoma and its effect in glycolysis.Methods TE13 cells and Eca109 cells were cultured under hypoxic condition(1 ％O2)for different hypoxic time(6,12,24 and 48 hours).Cells cultured under normal oxygen condition(20％O2)were set as control.The changes of HIF-1α and HK-Ⅱ expressions at protein level were detected by Western blot.HIF-1α genes were specifically silenced with RNA interference technology(RNAi),and then the changes of HIF-1α and HK-Ⅱ expression were determined by realtime PCR and Western blot.Under normal oxygen and hypoxic condition,the changes of lactic acid concentration in cell culture medium were detected by spectrophotometric method.Results Under hypoxic condition,the expression of HIF-1α and HK-Ⅱ gradually increased as hypoxic time extended(P＜0.05),reached a peak at 12h and then gradually decreased as time extended.Compared with that under normal oxygen condition,the expression of HK-Ⅱ in TE13 cells and Eca109 cells significantly increased under hypoxic condition(P＜0.05),which was more significant after 12 hours hypoxia.The result of realtime PCR indicated that under normal oxygen condition the expression of HIF-1α at RNA level in TE13/shRNA cells and Eca109/shRNA cells significantly decreased compared with TE13 cells and Eca109 cells without interference(P＜0.05).The expression of HK-Ⅱ at RNA level was consistent with the result of HIF-1α.Under normal and hypoxia condition,the expression of HK-Ⅱ at protein level in TE13/shRNA cells and Eca109/shRNA cells significantly decreased compared with TE13 cells and Eca109 cells without interference,and the difference was statistic significant(P＜0.05).The lactic acid secretion of TE13 cells and Eca109 cells under hypoxia condition(14.707 ± 3.594 and 15.062 ±3.901)was higher than that under normal oxygen condition(6.070±1.839 and 6.891±1.592,P＜0.05).The lactic acid secretion of TE13/shRNA cells and Eca109/shRNA cells significantly decreased compared with TE13 cells and Eca109 cells without interference,and the difference was statistic significant(P＜0.05).Conclusion The expressions of HIF-1α and HK-Ⅱ in human esophageal squamous cell carcinoma significantly increased under hypoxia conditions.The expression of HK-Ⅱ is closely correlated with lactic acid concentration and HIF-1α expression.HIF-1α may affect cell glycolysis through HK-Ⅱ.