Currently refractory and relapsed acute leukemia (AL) continues to develop a poor prognosis,and the treatment remains a significant clinical challenge.The use of priming regimen including granulocyte colony-stimulating factor(G-CSF) in clinic provides a novel method for the treatment of refractory and relapsed AL.This review summarizes the mechanism,method and efficiency of priming regimen in the treatment of all kinds of refractory and relapsed AL.

Objective To analysis the dependence factors of postpartum infection in our hospital trial cases in recent 5 years.To explore the reasonable parturition and improve the quality of obstetric measures.Methods A retrospective analysis of the incidence of infection after the trial was conducted, in which the clinical data of 106 patients were from June 2014 to June 1999,and compared with 212 cases without infection after parturition.Results In the trial of more than 48h,cesarean section,vaginal infection group were 85.8%,87.7% and 67.0%.They were higher than the control group 22.6%,36.8%and 35.4%.The difference between the two groups was statistically sig-nificant (P<0.05).Conclusion The trial parturition time is the leading cause of infection.In order to reduce the rate of cesarean section,to reduce maternal puerperal infection,subsequent complications such as uterine scar,strict control of indications for induction of spawning is needed.

Objective To discuss the security of negative HBsAg blood by correlation analysis between 5 markers of hepatitis B and HBV-DNA among blood donors with HBsAg negative.Methods 5 markers of hepatitis B were detected by ELISA and HBV-DNA by real-time PCR.Results In 3 398 samples of negative HBsAg blood donors,the total positive rate of HBV-DNA was 2.73%.Among them,the type series of HBsAb(+),HBcAb(+)account 6.59%,the positive rate of HBV-DNA was 5.80%;the type series of HBsAb(+),HBeAb(+),HBcAb(+)account 4.74%,the positive rate of HBV-DNA was 6.83%;the type series of HBcAb(+)alone account 6.24%,the positive rate of HBV-DNA was 5.19%;the type series of HBeAb(+),HBcAb(+)account 5.86%,the positive rate of HBV-DNA was 4.52%;the type series of HBsAb(+)alone account 31.99%,the positive rate of HBV-DNA was 1.56%;the type series of entire negative results account 44.59%,the positive rate of HBV-DNA was 2.11%.Conclusions Blood donors with negative HBsAg still have the risk of infecting HBV,especially when HBcAb is positive alone,or combining with other positive markers such as HBsAb,HBeAb,latent transfusion'infection,the risk was remarkably increased.As a result,we should detect the other markers of hepatitis B simultaneously and carry out real-time PCR technique to screen blood in order to reduce infection rate arising from transfusion.

Objective To explore the clinical value of diagnos is value of the dynamic EEG monitoring (AEEG) (24-hour EEG monitoring) in epilepsy diagnosis. Methods 252 cases of clinically diagnosed as epilepsy were received AEEG monitoring, interictal awake and sleep EEG epileptiform discharge detection rates were compared. Results Of 252 cases epileptiform discharges were detected in 191 cases. appear separately in the awake period in 17 cases,sleep alone in 80 cases,both awake and sleep there were 94 cases, 111 cases were found in a sober period(accounting for 58. 1%) ,sleep period was found in 174 cases(91.1%) ,the difference was statistically significant(P ＜0. 01). Epileptiform discharges were seen mainly in NREM sleep Ⅰ -Phase Ⅱ. Conclusion Epileptiform discharges during sleep was significantly higher than those detected in the awakening period, AEEG contribute to the diagnosis of epilepsy,seizures can not be excluded on clinical and conventional EEG in patients without positive findings,can be AEEG monitoring.

Objective To investigate the related factors and prevention measures of recurrent intracerebral hemorrhage in cerebral infarction patients with cerebral microbleeds.Methods 124 patients with acute cerebral infarction were divided into two groups according to the GRE -T2 * W1 examination,cerebral microbleeds as control group(n =42),non cerebral microbleeds as observation group(n =82);The recurrence of cerebrahemorrhage and related factors of the two groups were compared.Results In the control group,the distribution of the CMB in intracranial:thalamus -basal ganglia area was 23,accounting for 54.7%;cortical -subcortical area was 12,accounting for 28.5%,under the curtain area was 7,accounting for 16.7%.The incidence of recurrent intracerebral hemorrhage was 1 1 .3 % ,7.3% and 0.8% respectively .The incidence of recurrent intracerebral hemorrhage in the group with CMB(40.4%)was obviously higher than that of without the CMB group(10.9%),the difference was statistically significant(χ2 =11.263,P <0.05 ).Conclusion The cerebral infarction patients combined with CMB has high -risk in the aspect of recurrent intracerebral hemorrhage.Through the GRE -T2 * W1 to find CMB,which can guide clinical doctors choose reasonable treatment effectively,and then reduce the occurrence of cerebral hemorrhage and improve the prognosis of patients.

Telocytes (TCs) are a kind of interstitial cells recently identified,which distribute in the heart,lung,liver,kidney,brain and some other organs.These cells are characterized by a small cell body and extremely long and thin telopodes (Tp).The TPs arranged in moniliform aspect with alternation of thin segments and small dilations,forming a complex 3D network with the surrounding cells.More and more researches show that TPs have a role in injured tissue repairement directly or indirectly.This review focus on its function of tissue repair and the possible mechanism.

Objective The current study was aimed to establish an electrically amygdala kindling model of refractory epilepsy in Sprague-Dawley rats, and to study their changes of electrocorticagram ( ECoG ) .Materials and methods Male Sprague-Dawley rats were used in the experiment.Two-polar electrode was implanted into the right amygdala stereotactically.Two weeks after the surgery, electrical stimulations were given to elicit the grade 4 seizure in all animals with the fast kindling protocol.Rekindling was administrated after the first kindling.The after discharge threshold ( ADT) and the number of stimulus were reassessed in the two kindling protocols.ECoG and the behavior of the animals were recorded during the whole experiment.Result The changes of ECoG and behavior: Animals showed stage 1 -3 seizure when the ADT was assessed.While during the kindling period, the animals showed generalized convulsion with stage 4-5 seizure.ECoG showed sharp waves, spike waves, sharp-slow waves and spike-slow waves.Statistical analysis showed that the ADT was not significantly increased at two weeks after kindling ( from 83.33 ±22.29μA to 84.17 ±16.76μA, P=0.923 ) .The number of stimulus given to elicit the stage 4 convulsion was not significantly increased as well ( from 4.41 ±2.27 to 5.58 ±3.96, P=0.231 ) .Conclusions Rapid kindling model of epilepsy in rats is an effective epilepsy model, which is stable for 2 weeks.

Spasticity often results in motor dysfunction, lowering individual abilities of daily living, and limitation of social particitation of the patients, prolonging function recovery, deteriorating quality of life. Drug is helpful to improve spasiticity and reduce complications. But there are some adverse effects in cognition function. Individual medication should be recommended as proposal.

Objective:To explore therapeutic effect of rehabilitation intervention on patients with diabetes mellitus (DM)complicated tuberculosis (TB).Methods:A total of 108 DM + TB patients treated in our hospital from Jan 2008 to Aug 2013 were enrolled.According to random number method,they were randomly and equally divided into rehabilitation intervention group and routine treatment group.The intervention course was eight weeks,and then rehabilitation effect was compared between two groups.Results:Compared with before treatment,after treatment, there were significant reductions in fasting blood glucose (FBG)and 2h postprandial blood glucose (2hPBG)in both groups,P <0.01 all;compared with routine treatment group,after treatment,there were significant reductions in levels of FBG [(6.77±1.82)mmol/L vs.(5.54±1.46)mmol/L]and 2hPBG [(7.94±1.83)mmol/L vs.(6.87 ±1.42)mmol/L],P <0.01 both,and significant rise in percentages of significant reduced or disappeared pleural effusion (51.85% vs.83.33%)and significant reduced or closed TB focus (44.44% vs.72.22%)in rehabilitation intervention group,P <0.01 both.Conclusion:Rehabilitation intervention can make patients get better control of blood glucose,promote absorption of pleural effusion and pulmonary tuberculosis focus.

Objective To explore the significance of non-neoplasma touch and neoplasmacide technology on cancer of colon D_3 radical correction.Methods 67 cases of cancer of colon D_3 radical correction were selected from 1998 January to 2003 March.They were treated with non-neoplasma touch and neoplasmacide technology and their curative effect were compared with the control group treated with D_ 1～2 modus operandi and without non-neoplasma touch and neoplasmacide technology.Results In the treatment group,no complication appeared after the operation.All cases cured.After 3 years follow-up,the recurrence rates were 34.9% in the control group and 14.9% in the treatment group.It had significant difference(P