Objective To optimize extraction technology for volatile oil of Pogostemon cablin in the second traditional veterinary drugs anti-stress granule.Methods The single factor was used to investigate the concentration and amount of the ethanol before inclusion of volatile oil.The L9(34) orthogonal design was used to optimize the factors in the process of inclusion.Results The optimal of technology method was that volatile oil was dissolved with 95% EtOH by 1:20(mL/mL),the ratio of oil and ?-CD was 1:8,?-CD was prepared the concentration of 10%,ultrasound pool was at 40 ℃,2.0 h.Conclusion The optimized process is stable and adjustable quality.

6,7-dicho- methoxyl-8-Hydroxycoumarin. Fraxinus rhynchophylla Hance extract had obvious bacteriostasis to 9 kinds of bacterium;monomer mixture and coumarin fingerprint region sample had obvious bacteriostasis to Staphylococcus aureus, Staphylococcus epidermidis, beta streptococcus, aeruginosus Bacillus, Bacillus coli, Klebsiella pneumoniae, catarrh. Unknown component fingerprint region sample had obvious bacteriostasis to Staphylococcus aureus and certain bacteriostasis to Staphylococcus epidermidis, alpha streptococcus, beta streptococcus, Bacillus coli, salmonella. Conclusion The changes of ash bark bacteriostasis were basically identical with known coumarin component content. Aglycone bacteriostasis of ash bark was superior to glycoside, fraxetin and aesculetin bacteriostasis were most powerful in 5 kinds of coumarin monomer.

Objective To determine the content of berberine hydrochloride in Shaoyao decoction. Methods HPLC was performed on an Agilent XDB C18 column by using acetonitrile-0.05 mol/L potassium dihydrogen phosphate-triethylamine (30∶70∶0.14) as mobile phase at a flow rate of 1.0 mL/min, the column temperature was 25 ℃ and the UV detection wavelength was 345 nm. Results Berberine hydrochloride showed a good linearity redationship in the range of 0.07～1.40 ?g. The average recovery was 98.63% (n=6, RSD=1.21%). The average content was 1.55%. Conclusion The method is simple and accurate, with good repeatability, and can be used for the quality control of Shaoyao decoction.

It is one of key scientific problems to identify the effective compounds in the study on new TCM. Because of the integrity of the effects of TCM, the diversity of constituents, the complexity of the targets and the unpredictability of the interaction among the components, the efficiency of the traditional efficacy substance research method is generally not high. Based on the research thought of quantitative composition-activity relationship, there are inevitable quantitative correlation between chemical compositions and its biological activity. The quantitative composition-activity relationship research is an effective method to detect the effective compounds. This article reviewed the common methods and technologies used in the quantitative composition-activity relationship research, with a purpose to provide some references for the study on basic effective components in TCM.

Objective:To determine the antioxidization and antiproliferation of extract from leaves of Toona sinensis (LTS).Methods:The total phenolic extract of LTS was obtained by solvent and polyamide resin to determine the content.The antioxidization of the LTS extract was measured by TOSC assay.Antiproliferation was studied in vitro with different human cancer cells.Results:The total phenolic content in the LTS was (427.53±4.31) mg/g and antioxidization was 807.64 μmoL vitamin C equivalents/g in the sample.The extract significantly inhibited the colon cancer cell Caco-2,human liver cancer cell HepG2 and breast cancer cell MCF-7 proliferation with EC50 (4.00±0.39),(153.16±13.49) and (193.46±14.68) μg/mL,respectively.The bioactivity index (BI) of the LTS extract was nearly 283.Caco-2 was more sensitive than MCF-7 and HepG2.Conclusion:Extract from LTS has anticancer properties useful for preventing chronic diseases.

Objective To optimize the conditions for the extraction of volatile oil from Fructus Tsaoko and Radix Saposhnikoviae in Leifengguan granules,and for the preparation of their ?-CD inclusion compounds.Methods With the percentages of extracted volatile oil of Fructus tsaoko and Radix saposhnikoviae as the target marker,single-element experiments were conducted to determine the optimum conditions for extracting the volatile oil.The application rate of included volatile oil was used as the target marker to optimize the conditions for including the oils with ?-CD.Results The optimized extraction conditions were: with 8-fold water added,the sliced herbal medicine of Fructus tsaoko and Radix saposhnikoviae should be extracted for six hours without advanced soaking with water.The optimized inclusion conditions were: applying the saturated aqueous solution method,15-fold 85 % ethanol was used to dissolve the volatile oil,the proportion of the above solution and ?-CD being 1︰8;at 25 ℃,the volatile oil was included for 30 minutes with ultrasonic wave.Conclusion The established process can be used for the extraction and the inclusion of volatile oil from Fructus Tsaoko and Radix Saposhnikoviae in Leifengguan granules.

Objective:To establish the quality control method for Tribulus terrestris L. by colorimetry and HPLC. Methods:The HPLC method was with a Welch Ultimate LP-C18 column(250 mm × 4. 6 mm,5μm),the mobile phase was methanol-water(80:20) and the detection wavelength was 203 nm. The colorimetry was with a perchloric acid method. The saponins of Tribulus terrestrist as the index,the determination method for total saponins and saponins of Tribulus terrestris L. was established. Results:The results of the HPLC and colorimetry methods showed saponins of Tribulus terrestris had good linear relationship within the range of 0. 820-7. 380 μg and 24. 600-86. 100 μg with the average recovery of 99. 3% and 99. 5%,respectively. Total saponins and saponins of Tribulus terres-tris in Tribulus terrestris from 18 habitats were measured by the methods. Conclusion:The methods are sensitive,accurate and repro-ducible,and can be used as the quality control methods for Tribulus terrestris.

Objective To establish a UV spectrophotometry method and an HPLC method respectively for the determination of the total content of coumarin and contents of four main constituents of coumarin in Fraxini Cortex extract.Methods UV spectrophotometry was used for the determination of the content of total coumarin in Fraxini Cortex extract. The reference substance was Aesculin, and the maximum ultraviolet absorption wavelength was 334 nm. The HPLC method was used to determine the contents of Aesculin, Fraxin, Aesculetin and Fraxetin in Fraxini Cortex extract, using gradient elution with acetonitrile-phosphate solution (0.01%) as mobile phase on Agilent ZORBAX SB-C18 chromatographic column (4.6 mm × 250 mm, 5μm) at room temperature.Results For the UV method, the linear range of the mass concentration of Aesculin was 5.76-23.04μg/mL (r=0.999 9), and the average recovery was 100.6% (RSD=1.8%). For the HPLC method, the linear ranges of the mass of Aesculin, Fraxin, Aesculetin and Fraxetin were 0.055 0-3.850 0μg (r=0.9997), 0.053 9-3.773 0μg (r=0.999 8), 0.060 0-0.660 0μg (r=0.999 9), and 0.056 2-0.618 2μg (r=0.999 9), respectively, and the average recoveries were 96.97% (RSD=1.26%), 100.80% (RSD=2.22%), 99.04% (RSD=2.47%), and 98.77% (RSD=1.94%), respectively.Conclusion Both of the two methods are simple, accurate and reliable, and can be used for the quality control of total coumarin and the main constituents of coumarin in Fraxini Cortex extract.

Objective To establish a method to analyze the composition and content of monoses in the garlic polysaccharide by ion chromatography. Methods The monoses in the solution of garlic polysaccharide hydrolysised by trifluoroacetic acid were determined by amino PAC column, eluted by gradient hydroxide sodium and identified by ampere detector (AU as working electrode, Ag/AgCl as reference electrode). Results The garlic polysaccharide was mainly composed by arabinose, galactose, glucose, xylose and fructose. Good resolution could be achieved among the above mentioned five kinds of monoses. Their linear correlations over the investigated concentration were above 0.998, while the average recovery rates were in the range of 95%-105%. Conclusion The ion chromatography method is fast, accurate, simple and reliable, and can be applied in the content determination and quality control of garlic polysaccharide.

“Multi-component Chinese medicine” (MCC Medicine) is a new TCM concept in recent ten years. It is a new formed TCM product accepted and approved by the new mode (component compatibility of medicines) for TCM research and development, which originated from TCM research and development and TCM pharmaceutics. MCC Medicine contains massive historical accumulation of TCM and distinctive characteristics of the times, which is closely connected with the TCM theory, current trend of the TCM development, clinical treatment requirements, and the development of modern science and technology. In order to promote the development of MCC Medicine, this article reviewed its original background and future trend, with a purpose to make clear the direction for the development of MCC Medicine.