AIM: To estalish a HPLC method for the determination of cinobufagin and resibufogenin in Niuhuangxiaoyan Tablet(Calculus Bovls, Radix et Rhizoma Rhei, Concha Margaritifera Usta, Venenum Bufonis, etc.). METHODS: Waters Symmetry Shield RP18(3.9mm?250mm,5?m) column at 30℃ was used with a mobile phase consisted of acetonitrile -0.5mol?L -1 potassium dihydrogen phosphate(solution was adjusted by phosphoric acid to pH=3.2) (42∶58) and a UV detector at 296nm. The flow rate was 1.0mL?min -1 . RESULTS: The linear range of cinobufagin was 0.0696～0.8352?g. The RSD of measurement precision test was 0.53%; The average recovery was 100.06% ( RSD =1.25%, n =5). The linear range of resibufogenin was 0.1576～0.9456?g. The RSD of measurement precision test was 0.15%. The average recovery was 100.54%( RSD =1.49%, n =5). CONCLUSION: The method is simple and accurate, and can be used for the quality control of Niuhuangxiaoyan Tablet.

Objective To investigate the chemical constituents of Balanophora simaoensis. Methods Chromatography and spectra were used to isolate the constituents and elucidate their structures. Results Four compounds were isolated from whole plant of B. simaoensis and elucidated as methylconiferin (Ⅰ), butylconiferin (Ⅱ), 4-O-?-D-glucopyranosyl confieryl aldehyde (Ⅲ), and brevifolin (Ⅳ), respectively. Conclusion Butylconiferin is a new compound.

Chinese Caterpillar Fungus (CCF) is one of the rare Chinese traditional drugs. As the resource is reducing sharply, the price is rising higher and higher, and there have been much more adulterants in the markets, but until now we don't have a scientific and accurate research on the identification study for this drug. On the basis of resource investigation, during the study of the samples collected by ourselves and the specimens stored in the museum, using the macroscopic and microscopic methods, referring to the literatures of entomology, emphasizing on the characteristics of polypide part, we have studied this species in detail of the macroscopic characters such as the insertion position of the stroma part, the annulations and segments of the caterpillar, the abdominal leg, the pinaculum, and the microscopic characters of the body wall; firstly added the microscopic character of the crotchets on the planta of abdominal leg. The result turned out that the characters which we have studied are regular and stable, and it have laid the foundation for the powder products and patent medicines which have used the crude drug of CCF.

Object To study the chemical constituents in the ro ot of Isatis indigotica Fort Methods The s eparations were carried out by column chromatography and identified by advanced physical and spectral data analysis Results Five compounds were isolated and identified as neohesperidin (Ⅰ), ammonium formate (Ⅱ), isol iquiritigenin (Ⅲ), liquiritigenin (Ⅳ), and adenosine (Ⅴ) Conclusio n Neohesperidin, liquiritigenin and isoliquiritigenin are isolated f o r the first time from the plants of Cruciferae, ammonium formate is obtained fro m the root of I indigotica for the first time

Objective To study the chemical constituents of Phlomis medicinalis.Methods The constituents were isolated and purified by various modern chromatography techniques and the structures were elucidated by physicochemical properties and spectral analyses.Results Ten compounds were obtained and elucidated as 1-dehydroxyshanzhigenin methyl ester(Ⅰ),notohamosin A(Ⅱ),verbascoside(Ⅲ),3,4-dihydroxyphenyl ethanol(Ⅳ),succinic acid(Ⅴ),sucrose(Ⅵ),2,6-difructose(Ⅶ),butyl-?-D-fructopyanoside(Ⅷ),D-glucose(Ⅸ),and D-fructose(Ⅹ).Conclusion All the compounds are isolated from this plant for the first time,compound Ⅱ is obtained from the plants of Phlomis Linn.for the first time,and compound Ⅰ is a new one.

AIM: To study Cordyceps sinesis and its cultured mycelia by HPLC.METHODS: An HPLC established for the fingerprints of cordyceps sinensis and its cultured mycelia was performed on an Agilent C18(4.6 mm ? 25 mm,5 ?m)as column and acetonitrile-0.1% phosphoric acid solution in gradient as mobile phase.The flow rate was 0.5 mL/min,and the detection wavelength was set at 260 nm.RESULTS: There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia,three of which were determined as uracil,adenosine,cordycepin.CONCLUSION: The method can be used for the study of fingerprints of cultured mycelia.

Objective: In the paper, the principle of determining similarity of chromatographic fingerprints of TCM has been introduced. Methods: In the chromatographic fingerprint analysis of TCM, the whole fluctuation of the chemical composition of TCM products could be estimated by the comparison between their chromatographic fingerprints. Two methods, which determine the similarity between chromatographic fingerprints using peaks area or data points of chromatogram, have been investigated by the simulation and experiments. Results: The results both suggest that compared with the method for determining similarity using data points, the method using peaks area is more robust and its calculating results is more reliable. Conclusion: Peaks area method is more suitable for chromatographic fingerprint of TCM.

Objective To develop a HPLC method for the simultaneous content determination of three terpenoids inMelastoma dodecandrum Lour. (asiatic acid, betulinic acid, oleanolic acid).Methods The chromatographic column was set with waters SunFire C18 column (4.6 mm×150 mm, 5μm); the moving phase was acetonitrile -0.1%H3PO4; the column temperature was 30℃; the detection wavelength was 200 nm; the flow rate was 0.6 ml/min; and the sample volume was 25μl.Results A good linear relationship was observed in the range of 0.310-6.200μg (r=0.9999), 0.405-8.100μg (r=0.9999), 0.169-3.375μg (r=0.9998) for asiatic acid, betulinic acid, oleanolic acid respectively, with the average recovery rates of 102.08%, 101.81%, 102.22%. Conclusions The established method is convenient and sensitive, repeatability and stability, quality evaluation for Melastoma dodecandrum Lour.