Objective To investigative the relationship of personality and coping style with subjective well-being in nurses.Methods 140 nurses were invited randomly to answer the questionnaires.123 questionnaires out of 140 were selected,with 17 papers excluded because of un-complete answer.Personality,coping style and subjective well-being were respectively estimated with Eysenck Personality Questionnaire(EPQ),Simplified Coping Style Questionnaire(SCSQ) and General Well-Being Scale(GWB).The resuits were compared with the national norm.Correlation analysis was performed in term of the correlation of personality and coping style with subjective well-being.Results Subjective well-being of nurses was associated with personality and coping style.Conclusions Personality and coping style can predict subjective well-being.The research can provide theoretical basis for psychological care to the nurse group.

Objective: To investigate the association between the left ventricular hypertrophy (LVH) and blood pressure load (BPL) and circadian rhythm in hypertensive patients. Methods:Sixty patients with hypertension were studied by 24-hour ambulatory blood pressure monitoring ( ABPM) and colour Doppler echocardiography. Results: Daytime、 nighttime and 24-hour systolic blood pressure load (d-SBPL、n-SBPL、24h-SBPL) in hypertensive patients with LVH were significantly higher than those without LVH[(88.4?11.6) vs (79. 8?13. 4) ,P

OBJECTIVE: To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells. METHODS: Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. RESULTS: Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased. CONCLUSION: TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.

Objective To decrease the fault rate and increase the clinical application management of the defibrillator.Methods The allocation,training,daily maintenance,quality control,emergency treatment and etc of the defibrillators in some hospital were summarized,and a set of clinical application management method was proposed for the defibrillators.Results The allocation and maintenance of the defibrillators were improved,and the rate for the completion of records reached 100％.The fault rate of the defibrillators was 0,and A requirements for first-aid and life support devices in grade assessment issues were fulfilled.Conclusion It's proved the management method is feasible for eliminating the deficiencies in clinical application management of the defibrillator.

Normotensive primary hyperaldosteronism is rare. One case of this syndrome treated with alcohol injection was reported, and the clinical manifestation and laboratory data were analysed and discussed.

Objective:To explore the method of building the formative assessment (FA) system in diagnostics teaching and investigate its implementation effect.Methods:We planned and formulated the formative assessment system in diagnostics teaching of undergraduate curriculum. The correlation between the scores of the formative assessment and final exam were analyzed. A questionnaire survey on students' attitudes towards FA and an interview on teachers' perspectives were conducted. SPSS 17.0 was used for statistical analysis. Linear correlation analysis was used to compare the final exam scores with formative assessment scores, and Wilcoxon rank sum test was used to compare the scores of questionnaire items.Results:The scores of FA were significantly positive related to the scores of final exam ( r = 0.559, P < 0.001). The questionnaire analysis showed that students significantly valued the formative assessment superior to the summative assessment ( Z = -8.632, P<0.001). It was generally approved of the promotion of FA in students' self-direct learning and self-supervision and the advances in acquirement of knowledge and skills. Conclusion:It is feasible of carrying FA in diagnostics teaching which could provide better learning outcome and might have an extensive prospect of application.

ObjectiveTo investigate the alteration of behavior,such as spontaneous movement,spatial memory ability and cholinergic M receptors in the brain of mice subjected to chronic mild stress (CMS). And to determine whether Ningshen Ling (NSL)and dehydroepiandrosterone(DHEA)could reverse the cognitive impairment in this model.MethodsForty mice were divided into four groups: control group,CMS group,CMS+NSL(p.o.5g·kg-1·d-1) group and CMS+DHEA (i.p.5 mg·kg-1·d-1) group. Morris water maze procedure was used to determine the spatial memory ability. M receptor binding activity was measured with radioactivity assay by3 H-QNB.ResultsAfter 3 weeks CMS,the mice exerted a decrease in spontaneous movement test,and the latency in Morris water maze was obviously prolonged. Treating CMS mice with NSL and DHEA for 1 weeks could improve the spontaneous movement and latency declined. Whereas the3 H-QNB binding ability to M receptor showed a significantly decrease in cerebral cortex and hippocampus of CMS mice (P<0.05),the decreased ability of M receptor binding was reversed by NSL treatment in hippocampus(P<0.05).ConclusionNSL and DHEA can alleviate the stress response and reversed cognitive impairment induced by CMS,and it may be concerned with the central cholinergic M receptors activity.

To assess and grade facial nerve dysfunction according to the extent of facial paralysis in the clinical course of acupuncture treatment for Bell's palsy, and to observe the interrelationship between the grade, the efficacy and the period of treatment, as well as the effect on prognosis.

Background Pupillary light reflex has been widely used in the diagnosis and evaluation of visual system and nervous system diseases.However,in animal experiments,functional evaluation of the visual system and nervous system needs more advanced technology and are affected by many factors.Objective This study was to explore the use of the dynamic pupillometer in evaluating pupillary light reflex and to discuss the influence of brightness of stimulate on relevant curve parameters in C57BL/6 mouse.Methods Ten healthy SPF male C57BL/6 mice were collected in this experiment.White light of five luminance levels (2,8,32,128,256 cd/m2) was used to stimulate the mice following a 2-hour dark adaptation.The stimulation was given at the 60-second intervals,for a duration of 100 ms at every stimulation.An infrared camera and video capture card were used to capture digital images during the measuring process in a scotopic environment,at a speed of 60 frames per second.Measuring outcome was saved in the*.AVI format.A software that was developed by our group was used to determine pupil diameter and output pupillary light reflex curve offline.Pupil initial diameter (R1),constriction amplitude (CA),constriction velocity (CV),latency (T1),time for maximum velocity (T2),time for maximum constriction (T3),time for maximun con-striction to 10.1％ R1 re-dilation (RT)and re-dilation velocity (RV)were assessed,and the correlations between luminosity and measuring parameters were analyzed using the Spearman rank correlation.The use of animals followed the Regulations for thd Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission.Results R1 values showed no statistically significant difference among the 5 different luminosity groups(F=1.117,P=0.361).A positive linear correlation was found between stimulating luminosity and CA(r=0.508,P＜ 0.01),but negative correlations were seen between stimulating luminosity and CV or RV (r=-0.625,-0.609,P＜0.01).T1 and T2 values in the 5 different luminosity groups were not statistically significant (F =0.202,P =0.936 ; F =1.584,P =0.195).The different levels of stimulating luminosity showed positive linear correlations with T3 and RT values (r =0.791,0.609,P＜ 0.01).Conclusions The dynamic pupillometer can quantitatively measure the pupillary light reflex of C57BL/6 mice.The pupillary light reflex dynamic curve parameters of mouse were affected by stimulus luminosity levels.These outcomes offer a basis for the application of the dynamic pupillometer system for measuring pupillary light reflex in animal models.

To improve the effect of pediatrics clinical probation teaching for eight-year program medical students,the exploration and practice of case-based instruction teaching with symptoms as main line,reading reports,and application of high quality counterfeit baby simulator-assisted instruction were carried out,which could inspire students' learning interest,and contribute to the training of students'clinical and scientific thought,their self-education and clinical skills.