The condition and prognosis of ischemic cerebrovascular disease(ICVD)are closely associated w ith the w hole blood coagulation status and the reactivity of antiplatelet agents of patients. Thromboelastography ( TEG ) is a reliable means to detect the w hole blood coagulation and fibrinolytic capacity. Its overal results of the evaluation can be used in the coagulation monitoring, efficacy of antiplatelet therapy, and prognostic evaluation in patients w ith ischemic cerebrovascular disease. In recent years, the detection methods of TEG have been improved continuously. The research reports for its clinical application are gradual y increasing. This article review s the clinical application of TEG in the diagnosis, treatment, and prognostic evaluation in ischemic cerebrovascular disease.

Objective:To establish the pathological sample database and standardize pathology specimen management processes to provide a platform for the study of pathology samples. Methods: Using J2EE, JSP, and component object model technology, the system establish the pathological sample library work platform for the pathology network resources, documentation, and intelligent. Results:The system established pathology samples and workflow management system architecture for the collection, storage and analysis of pathology specimens. Conclusion:The system designed to enhance the authenticity and credibility of the sample test results, thereby improve the accuracy of the sample analysis and provides a good reference for information technology-related departments.

Objective To report the first case of ectodermal dysplasia/skin fragility syndrome in China. Methods The clinical data, transmission electron microscopic examination and immunohistochemical analysis of this patient were evaluated. Results The patient was a 3-year-old girl who presented increased skin fragility with trauma-induced, blisters and erosions, combined with short and sparse hair, finger-and toe-nail dystrophy and palmoplantar keratoderma. Transmission electron microscopy showed a loss of keratinocyte-keratinocyte adhesion, widening of intercellular spaces and a reduced number of hypoplastic desmosomes. Immunohistochemical analysis revealed a complete absence of staining for plakophilin 1. Conclusion Ectodermal dysplasia/skin fragility syndrome is a rare autosomal recessive inherited disease characterized by clinical manifestations as well as electron microscopic features and immunohistochemical evidence.

To evaluate the role of nitric oxide in local function of endometrium, the pattern of expression of endothelial and inducible nitric oxide synthases in the human endometrium at proliferative and secretory phases and decidua was studied by using immunocytochemistry. Results showed: (1) At the proliferative phase, the eNOS immunostaining was confined to vascular endothelial, whereas the iNOS was not detected in any composition of endometrium. (2) At the secretory phase, surface epithelium and grandular epithelium showed positive staining for both eNOS and iNOS. The stroma remained uniformly negative throughout the menstrual cycle. (3) In the decidua, the expression of both isoforms was increased, while moderate iNOS immunoreactivity was observed in decidualized stromal cells. It was demonstrated that the expression of eNOS and iNOS was elevated at the secretory phase and in decidua, indicating the increased production of NO at these phases. The increasing of NO might take part in implantation through dilating the vessels and relaxing the smooth muscles and in menstration through promoting apoptosis.

To evaluate the role of nitric oxide in the embryo implantation in mice, three experiments were carried out using the mice implantation model. In the experimentⅠand experimentⅡ, NG-nitro-L-arginine methyl ester (L-NAME), the non-specific NOS inhibitor, was administered intraperitoneally at doses of 0.5 mg-5 mg on the day 3 of pregnancy and at dose of 3 mg on different days of pregnancy (day 1-6) . In the experiment Ⅲ, L-aragnine, a donor of NO, was co-administered with L-NAME to evaluate the effect of L-aragnine on L-NAME, and the embryo development was assessed. In all these three experiments, the endometrium was histologically examined. Results showed compared with the control groups intraperitoneal administration of a dose of L-NAME between 1 and 5 mg on the day 3 of pregnancy led to the decrease in the number of implantation sites (P<0.05), and 4 to 5 mg of L-NAME caused inhibition of implantation completely. L-NAME resulted in failure of pregnancy when administrated at 3 mg between day 3 and 5 of pregnancy. The characteristic vascular permeability changes and decidualization in the endometrium were significantly attenuated and embryo growth was retarded. The L-NAME-mediated effects were significantly reversed when L-aragnine was co-administered with L-NAME. This study demonstrated that NO promoted the implantation in mice through regulating the permeability and decidulization of endometrium and the development of embryo.

Penile vascular endothelial damage, vasomotor dysfunction, and blood flow deficiency are the major causes of erectile dysfunction (ED). Current management of ED mostly depends on selective phosphodiesterase type 5- (PDE5) inhibitors, which fail for some ED patients. For Peyronie's disease-induced ED, surgical and physical therapies are used in addition to PDE5I medication, but frequently it is difficult to achieve satisfactory results. Recent studies show that the low-intensity extracorporeal shockwave therapy can promote angiogenesis and improve blood flow to the penis, which promises to be a novel effective therapy for ED and Peyronie's disease.
Erectile Dysfunction ; therapy ; High-Energy Shock Waves ; therapeutic use ; Humans ; Male ; Penile Induration ; therapy

One of the major responsibilities for medical students is to save lives and to popularize emergency medical treatment knowledge for the masses of society.Based on research analysis,systemic training of cardiopulmonary resuscitation program including lectures,simulation education and social practice were performed for medical students.After practice activities,these students could proficiently apply the basic knowledge and skills of cardiopulmonary resuscitation in practical work and spread what they have learned to the public.Furthermore the program could improve students' abilities in social practice and team spirit and it is of great social significance.

Urinary tract infections remain the most frequent infection in patients with either traumatic or nontraumatic spinal cord inju-ry. The urinary tract infections are closely related to impaired bladder storage, voiding dysfunction, and use of indwelling catheters after spi-nal cord injury. Repeated urinary tract infections can cause upper urinary tract deterioration, and related chronic or repeated exposure to the antibiotics used to treat urinary tract infections increases the risk of multidrug-resistant bacteria colonization. Therefore, it is important to de-termine appropriate urinary tract infections treatment in spinal cord injury patients who are admitted to rehabilitation hospitals. The limita-tions of the usual measures in prevention of urinary tract infections in this population have led the way to explore more innovative modali-ties and approaches. We reviewed the researches of urinary tract infections on spinal cord injury. It is presented to provide reference for clini-cal diagnosis and treatment.

Objective To investigate the relationship between serum plasma brain natriuretic peptide ( BNP ) levels and myocardial injury in neoborns after asphyxia. Methods Neoborns who were admitted to Department of neonatology, Zhongnan Hospital, Wuhan University from December 2012 to December 2013 within 3 days after birth were considered. According to the number organized in chronological order every other case, newborns with neonatal asphyxia were assigned to observation group. The observation group were further divided into myocardial injury subgroup and non-cardiac injury subgroup according to the diagnostic criteria of myocardial injury. Newborns without neonatal asphyxia or neonatal cardiovascular diseases were assigned to control group. Exclusion criteria for control group were electrolyte disturbance, liver and kidney dysfunction. Blood sample was drawn from patients within 2 hours of admission to hospital and again on day 14. Serum BNP , creatine kinase isoenzyme ( CK-MB) , serum sodium and calcium were detected for further analysis. Results In 107 cases with neonatal asphyxia, 77 infants who had complete clinical records were selected as observation group, of which 36 met the diagnostic criteria of myocardial injury and assigned to myocardial injury subgroup. Non-cardiac injury subgroup consisted of the rest 41 cases in observation group. Twenty-two cases were enrolled to control group. Within 2 hours after admission, the serum BNP level of myocardial injury subgroup were significantly higher than those of the non-cardiac injury subgroup and the control group ( 2. 35 ± 0. 44 , 2. 12±0. 64, 1. 88±0. 27, log transformed, respectively, P＜0. 05). The BNP level of non-cardiac injury subgroup were also significantly higher than those of the control group. Serum BNP and CK-MB levels of observation group were positively correlated (r=0. 212,P=0. 030). After treatment, serum BNP level of myocardial injury subgroup at 14 days after admission decreased significantly, compared to the level at 2 hours within admission (P＜0. 05). When the cutoff value for infants with myocardial injury was 108. 05 pg/mL, the area under the ROC curve was 0. 753, with a sensitivity of 75. 0％ and a specificity of 64. 5％, positive predictive value was 56. 4％ and negative predictive value was 72. 3％. Conclusions Serum BNP level can reflect myocardial injury in neonates with asphyxia and can guide clinical treatment.

Objective To investigate the detection rate of IAD or IAT and the degree of depression,anxiety and self-conscious-symptoms of IAD or IAT cases in adolescent students,and to analyze the relationship between IA and mental health.Methods 8 694 students from 9 schools in Tianjin by systemic stratified random sampling were investigated on the conditions of internet using and state of psychological and body health with questionnaire designed by ourselves.Result Among the students with internet using,the detection rate of depression was 24.47%.The detection rate of depression in students using internet is 24.47%.IAD,IAT and Non-IA are 32.16%,26.83%,23.17%,respectively.The detection rate of anxiety in students using internet is 3.32%.IAD,IAT and Non-IA are 12.13%,3.72%,2.26% respectively.There was a linear correlation of IA with the degree of depression and anxiety(P