1.Progress on exosomal transmission of microRNAs in cancer research
Chinese Journal of Clinical Oncology 2016;43(10):442-445
Exosomes are cell-derived vesicles that contain protein and RNA from the same source and function. The vesicle has a diam-eter between 30 and 100 nm. Exosomes are the natural carriers and have been recently used as drug delivery system for cancer treat-ment. MicroRNAs (miRNAs) function in RNA silencing and regulation of gene expression. They can exist as intracellular and extracellu-lar miRNAs. Extracellular miRNAs can function as secreted signaling molecules that affect receptor cell phenotype. They can also re-flect molecular changes in donor cells. Therefore, extracellular miRNAs can be potentially used for diagnosis and therapeutics. Studies show that the volume of exosomes in cancer patients' blood is higher than that in normal controls. The ability to package cancer-relat-ed miRNAs is a biological function of exosomes. In conclusion, specific miRNAs transferred by exosomes may play an important role in the pathogenesis of tumor or cancer. This paper presents a summary of research on exosomes as the carrier of miRNAs in the develop-ment and treatment of cancer.
2.A systematic review of the mechanisms of acupuncture for myocardial ischemia on central nervous system
International Journal of Traditional Chinese Medicine 2017;39(5):472-474
Based on the systematic review on acupuncture improving the mechanism of central nervous system in animal model of myocardial ischemia in recent years, we analyzed the effects of acupuncture on medulla oblongata rVLM zone, hippocampus, hypothalamus, spinal cord segment and automalic to the mechanism of positively regulating myocardial ischemic, which provided a new way for the clinical treatment of myocardial ischemia and cardiovascular diseases.
4.Measurement of recombinant human glucagon-like peptide-1(7-36) concentration by ELISA kit methods
Zhuanjie ZHENG ; Yi FANG ; Rui WANG
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
AIM: To qualitatively detect the concentration of recombinant human glucagon-like peptide-1(7-36)[rhGLP-1(7-36)] in human blood.METHODS: RhGLP-1(7-36) concentrations of standard and blood samples were detected by ELISA kits.RESULTS: The parameters of specificity,sensitivity,detection limits,reproducibiltiy and recovery can meet the requirements for pharmacokinetic studies.CONCLUSION: The concentration measuring method of rhGLP-1(7-36) in blood samples using ELISA kit produced by LINCO company can meet the detection methods of biological production,which can be used as a measuring method of rhGLP-1(7-36) concentration and for its pharmacokinetic studies.
5. Isolation and expression characteristic of cyp71av1 promoter from Artemisia annua
Chinese Traditional and Herbal Drugs 2011;42(4):765-769
Objective: Trying to find the ways to enhance the expression of cyp71av1 gene encoding cytochrome P450 mono-oxygenase which is a key enzyme in artemisinin biosynthesis pathway accelerating the artemisinin synthesis, the promoter of cyp71av1 was isolated and characterized. Methods: 5′ untranslated regions of cyp71av1 were isolated from Artemisia annua with thermal asymmetric interlaced PCR. For functional characterization, the isolated fragments were fused with β-glucuronidase GUS reporter gene and introduced into Nicotiana tabacum by Agrobacterium-mediated transformation. The GUS expression regulated by 5′ untranslated regions of cyp71av1 in transgenic N. tabacum under the normal or stressed conditions were detected by histochemical staining and quantitative spectrophotometry assay. Results: Two DNA fragments upstream of cyp71av1 coding sequence, a long fragment and a truncated fragment, were isolated from A. annua and introduced into N. tabacum respectively. Histochemical staining showed that two isolated fragments confered stable GUS expression in transgenic plants, and no significant difference was found between the two fragments on the GUS activity. The quantitative results also showed that the GUS activity in transgenic tobacco plants treated by dehydration, low-temperature (4 °C), and ultraviolet irradiation were 1.4 to 2.7 folds higher than that in the controls. Conclusion: It suggests that the isolated fragments has promoter activity and may be responsive to adverse environmental stresses.
6.Research on the diagnostic values of the detection of urine exfoliated cells by FISH and cytologic technology in bladder urothelial tumor
Wei CHEN ; Huixia YI ; Rui ZHANG
International Journal of Laboratory Medicine 2014;(22):3094-3096
Objective To compare the diagnostic values of the detection of urine exfoliated cells by FISH and cytology technolo‐gy in bladder urothelial tumor .Methods The combination probes of CSP3/CSP7 and GLPp16/CSP17 were both used in the FISH detection of urine exfoliated cells from suspected patients with bladder urothelial tumor .The urine exfoliated cells were detected by cytology technology at the same time .The sensitivity and the specificity of the two methods were compared .Results The sensitivi‐ty and specificity of FISH for bladder urothelial tumor screening were 92 .5% and 85 .0% respectively ,and those of cytology tech‐nology were 27 .5% and 90 .0% respectively .The sensitivity of FISH was significantly higher than that of cytology technology (P<0 .05) ,however ,the specificity differences between FISH and cytology technology were not statistically significant (P>0 .05) .Conclusion FISH is expected to become a new method for the screening of bladder urothelial tumor .
7.Expression of MAGE-10 genes in human hepatocellular carcinoma and its clinical significance
Haitao ZHAO ; Jingan RUI ; Yi ZHAO
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To study the relationship between the expression of MAGE-10 genes and the clinical parameters of metastasis and recurrence of patients with Hepatocellular carainama (HCC). Methods The expression of MAGE-10 genes in tumor tissues,liver tissues adjacent to tumor and hepatocirrhosis tissues was examined by RT-PCR method.The relationship between positive expression rate of MAGE-10 genes and clinical parameters of metastasis and recurrence (such as AFP, AFU, Anti-HCV, HBsAg, AFPmRNA, diameter of tumor) in patients with HCC were studied. Results The positive expression rate of MAGE-10 genes significantly higher in tumor tissues than that in the surrounding tumor tissues (51 6% vs. 0%, ? 2=18 95,P 0 05).There was no expression of MAGE-10 in hepatocirrhosis tissues of 10 cases. Conclusions There was a high frequency of MAGE-10 genes expression in HCC,but it is unrelated to the tumor markers,metastasis and recurrence of HCC.
8.Reversal Action of Acupuncture on Experimental Rats with Insulin Resistance
Wei YI ; Nenggui XU ; Rui JIN
Journal of Guangzhou University of Traditional Chinese Medicine 2001;0(03):-
[Objective] To observe the reversal action of acupuncture on experimental rats with insulin resistance. [Methods] Twenty-four rats were randomized into normal group, control group and treatment group. Each group had 8 rats. Rat models with insulin resistance were induced by feeding of high-carbonhydrate forage. Fasting blood-glucose (FBG) and plasma insulin (PI) levels and insulin sensitivity index ( ISI) were examined after acupuncture of Neiguan ( PC6 ) , Zusanli ( ST36) , Sanyinjiao (SP6) and Shenshu (BL23). [Results] FBG and PI levels were increased and ISI decreased in model group as compared with those in normal group ( P
9.Application and Proposals of X-ray Field Cabin in Earthquake Relief in Wenchuan
Xiaohui WANG ; Rui YI ; Yibing DU
Chinese Medical Equipment Journal 2004;0(09):-
Field cabin,a new generation of our military medical support system,was used in the Wenchuan Earthquake relief for the first time. The ordonnance and features of X-ray field cabin were also laid out. In Mianyang city,within 86 days,there were 710 wounded persons were checked up,and the rate of high quality photo was up to 85%. The rational ordonnance,steady performance,easily deployed and withdrawn features of the X-ray field cabin ensure it adapted to long-distance medical support and fulfill its mission effectively. However,some disadvantages of it are also found in the application,and innovating proposal is further introduced.
10.Influential Factors of Hearing Rehabilitation of Prelingually Deafened Children with Multichannel Cochlear Implant
Yi-qing CHEN ; Mo LONG ; Rui HAN
Chinese Journal of Rehabilitation Theory and Practice 2006;12(7):593-595
ObjectiveTo analyze the relative influential factors of hearing rehabilitation of the deafened children with multichannel cochlear implant. Methods42 prelingually deafened children who accepted multichannel cochlear implant were evaluated with their hearing ability, hearing thresholds and talent level, while their family and usage of the multichannel cochlear were investigated. Results and ConclusionThe result shows that the factors influencing the hearing rehabilitation are the occupations of the parents, income of the family, the time between diagnosing deaf and the operation, the time after the cochlear implant operation and the talent level. The results of the logistic regression show that the deafened children can gain their hearing developing rapidly if they living in a family with high income, their mothers have accepted more education, and they accepted longer time of continuing hearing-aid, etc.