Objective:To observe the clinical curative effect of narrow-band UVB-LED (308nm) combined with tacrolimus, narrow-band UVA-LED (365nm) combined with 8-Methoxypsoralen irradiation in the treatment of vitiligo.Methods: Sixty-four cases of vitiligo patients were chosen and randomly divided into control group and observation group, each group with 32 cases. The control group received 308nm narrow-band UVB-LED irradiation combined with tacrolimus,the observation group received 365nm narrow-band UVA-LED irradiation combined with 8-Methoxypsoralen, the time of appreance of the first pigment island in patients were compared between the two groups,the clinical curative effect of both groups were observed when treated with 10 times and 20 times.Results: The average irradiation times when first pigment island appreance in the observation group and the control group were 5.59 and 6.24 respectively, the difference was not statistically significant (t=1.772,P>0.05). The efficiency rate of the control group was 37.50% after 20 times treatment, and in the observation group was 40.63%, the difference was not statistically significant (x2=0.000,P>0.05).Conclusion: 308nm narrow band UVB-LED combined with tacrolimus and 365nm narrow-band UVA- LED combined with 8- Methoxypsoralen irradiation in the treatment of vitiligo have significant treatment effects, how to improve the treatment effect still need further study.

Nephroblastoma is the most common pediatric renal tumor.The research progress of gene mutations in Wilms tumor children is reviewed in this paper.The WT1 gene,CTNNB1 gene and WTX gene plays a role in onset of Wilms tumor,and the MYCN gene,P53 gene and SIX1 gene may be associated with the prognosis.

The intestinal mucosa is the biggest lymph organ in human body, which consists of the first line of defensive system with the respiratory mucosa and urinary mucosa. The intestinal mucosa is responsible to defend bacteria, viruses, dietary pathogens, non-steroid anti-inflammatory drug and so on. If the intestinal mucosal immune system is destroyed, the human will suffer many diseases such as infectious diseases,autoimmune diseases. Therefore, it plays an important part in physical health. T helper type 17 (Th17) cells different from Th1 cells and Th2 cells are a distinct lineage of T cells. The discovery of Th17 cells has ruined the constitutional pattern of Th1/Th2. Th17 cells, which are important parts of intestinal mucosal immune system,play critical roles in intestinal autoimmune diseases, tissue inflammation and the infection of various pathogenic bacteria. This paper will review the differentiation of Th17 cell and its function in intestinal mucosal barrier,intestinal autoimmune diseases and intestinal infectious diseases.

Objective To find out about the use of anti-infectious agents by tumor outpatients of the hospital. Methods A survey was conducted on 1 011 prescriptions for anti-infectious agents written out in the hospital from October to December of 2003 and an analysis was made of the structure and rationality of their use by means of drug utilization studies. Results The utilization rate of anti-infectious agents was 8.35% , accounting for 1.09% of the total sum of money for the prescriptions. The kinds of anti-infectious agents used were mostly cephalosporins and quinolones. The utilization rate of agents of compatibility was 4.44%. The drug utilization index of some chemicals was close to 1.00, accounting for about 44.44% . Conclusion There is no widespread abuse of anti-infectious agents by the outpatients of the hospital and the utilization rates of anti-infectious agents and agents of compatibility are low. Still, there are some problems in the use and compatibility of certain agents and the rationality of their use is to be questioned. The use of anti-infectious agents should be planned in a scientific way and in line with the economic condition of the patients.

Snake venoms are complex mixtures containing many different biologically active applications in the field of cancer research. In this paper, not only unpurified fraction, but also highly purified snake venoms are discussed,such as rhodostomin, acutobin, agkistrodon halys antithrombotic enzyme, batroxobin. The review mainly introduced the inhibitory effects of snake venoms on tumer metastasis.

Before irreversible neuronal damage occurs, quickly and securely recanalize vessels and restore blood flow to save the ischemic penumbra tissue are the main therapeutic target for acute ischemic stroke (AIS). Currently, the method for recanalization in early AIS is intravenous thrombolysis, how ever, the effect of intravenous thrombolysis is often not satisfactory because of the reasons, such as its short treatment time w indow , low vascular recanalization rate, and high incidence of bleeding complications. In recent years, w ith the research and development of mechanical thrombectomy device and the development of interventional technology, the therapeutic time w indow of AIS has been significantly prolonged, the vascular recanalization rate has increased significantly, the clinical outcome has improved significantly, and the treatment of mechanical thrombectomy has show n good application prospects.

Objective To summarize the experience in nursing 2 patients with anti-glomerular basement membrane disease.Method The nursing measures such as close observation of disease condition,careful nursing of medication,nursing during the treatment of double filtrated plasmapheresis and mental care to the patients and their family members.Results After treatment,the concentration of anti-GBM antibody was declined and the level of serum creatinine was also decreased.The renal function got rccovered to a certain extent.Conclusion Such nursing points as close observation of renal and pulmonary pathgenesis,timely and accurate administration of medicine according to doctor's orders and active observation and prevention of complication from double filtrated plasmapheresis are key.

Breast conserving surgery has become the first choice for the treatment of early breast cancer.However,the safety margin is still one of the important issues for surgeons in the implementation of breast conserving surgery.Because variety of clinical factors have some effects on the safety margin,at present,no consensus on safe margin of breast conserving surgery is reached,and the controversy still goes on.

OBJECTIVE: To prepare methazolamide eye drops and establish its quality control method.METHODS: Methazolamide eye drops was prepared using inorganic nanoparticles as carrier and methazolamide as principal agent.The content of methazolamide was determined by ultraviolet spectrophotometry.The stability of the preparation was investigated. RESULTS: The preparation was white suspension andin conformity with the description ofChina Pharmacopeia(2005 edition) inidentification and test.The linear concentration range of methazolamide was4~12?g.mL-1(r=0.999 9) and the average recovery was100.10% (RSD=1.11%).Methazolamide eye drops was unstable under highlight , while all the test items had no significant change in6-month accelerated test.CONCLUSION: The preparative method is si mple and feasible and the quality of the preparationis stable and controllable.But the preparation should be stored away formlight.

Objective To investigate the correlation between CD40-1C/T gene polymorphism and serum soluble CD40L (sCD40L) expression in cerebral infarction.Methods According to the inclusion and exclusion criteria,select the acute large artery atherosclerosis in patients with cerebral infarction as the case group,select the same period without a history of stroke examination subjects as the control group.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology was used to detect CD40 gene polymorphism and sequencing,double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of serum sCD40L.Results A total of 209 patients with large artery atherosclerotic cerebral infarction (case group) and 87 subjects without history of stroke (control group) were included.The CC genotype (31.6％) and C allele frequency (53.8％) of the case group were significantly higher than that of the control group (17.2％,39.1％) (x2 =6.94,10.69,P ＜0.01).Case group serum sCD40L expression level was significantly higher than those in the control group [(3.97 ± 1.20) vs (2.69 ±0.88)] (t =10.19,P ＜0.001).Case group serum sCD40L expression level was different among CC,CT,and TT genotypes (F =19.22,P ＜0.001),serum sCD40L level (4.55± 1.16) of CC genotype in patients was significantly higher than that of CT (3.93 ± 1.17) and TT genotypes (3.27 ± 0.90),serum sCD40L level (3.93 ± 1.17) of CT genotype in patients was significantly higher than the TT genotype (3.27 ±0.90).The serum sCD40L expression level of the control group in the CC,CT,TT has no statistically significant differences among various genotypes [(2.91 ±0.79),(2.67 ±0.89),(2.61 ±0.91),F =0.619,P =0.541).Conclusions CD40-1C/T gene polymorphism is associated with serum sCD40L expression level in cerebral infarction,serum sCD40L level of the CC genotype was significantly higher.