Combined with the partial least square regression, the oc tane number (RON), density, temperature evaporate (50%,90%), aromatics and olefi n concentration of gasoline were determined by the Fourier transform infrared spe ctroscopy at the wavenumber region of 1550～660cm－1. The experimenta l results showed that the actual values measured by the standard method were agre e with the prediction values by the proposed method.

OBJECTIVE To investigate the prevalence of meticillin-resistant Staphylococcus (MRS) isolates in Staphylococcus auricularis and S. hominis and detect their drug resistance. METHODS MRS isolates were detected by cefoxitin disc test and susceptibilities were tested by agar dilution method. RESULTS 94.7% and 91.7% MRS isolates were in S. auricularis and S. hominis. MRS strains were resistant to most of antimicrobial agents. There were no isolates resistant to vancomycin and teicoplanin. CONCLUSIONS MRS isolates are highly prevalent in S. auricularis and S. hominis with resistance to most of antimicrobial agents.

OBJECTIVE To investigate the prevalence of meticillin-resistant Staphylococcus haemolyticus(MRSH)and resistance in S.haemolyticus isolates. METHODS MRSH was detected by cefoxitin disc test and susceptibilities were tested by agar dilution method. RESULTS There were 86.4% of MRSH isolates.MRSH was highly resistant to penicillin,cefazolin,cefuroxime,ceftriaxone,tetracycline,ciprofloxacin,and clindamycin.The resistance rate to amikacin,rifampicine and chloramphenicol was 16.9%,11.2% and 28.1%,respectively.All isolates were susceptible to vancomycin and teicoplanin.Except for tetracycline,amikacin,rifampicin,and chloramphenicol,the resistant rate to other antimicrobial agents was significantly higher in MRSH than in MSSH. CONCLUSIONS MRSH is mostly occupied in S.haemolyticus isolates and resistant to most of antimicrobial agents.More attention should be paid to survey and detect these strains.

Objective To study the relationship between the expression of telomerase activity in human gastric cancer and the development of gastic cancer.Methods Telomerase activity in 45 samples from primary gastric cancer and 40 samples of "normal" gastric tissues was examined using in situ hybridization technique.Results Telomerase activity was positive in 38 of 45 gastric cancer tissues.Telomerase positive expression in advanced stage gastric cancer was 93 3%,which was significiantly higher than that in early stage(66 6%)(P

OBJECTIVE To investigate pathogens and their resistance in infective endocarditis patients.METHODS Pathogens and their resistance were detected by MicroScan WalkAway-40 system.RESULTS Most of pathogens were Streptococcus pyogenes and Staphylococcus.The resistant rate of S.pyogenes to tetracycline,erythromycin,ampicillin,penicillin,gentamicin and chloramphenicol was 78.6%,42.9%,14.3%,0,0 and 0,respectively.Four were MRS in 10 Staphylococcus isolates and they were resistant to most antimicrobial agents except vancomycin and higher than MSS.CONCLUSIONS Most of pathogens are S.pyogenes and Staphylococcus in infective endocarditis patients;most S.pyogenes isolates are resistant to tetracycline,erythromycin,but susceptible to penicillins;MRS are resistant to most of antimicrobial agents except vancomycin.

Objective:To investigate the effects of three herb extracts on cell proliferation and collagen synthesis in cultured hu-man dermal fibroblasts. Methods:The human dermal fibroblasts were isolated and cultured with tissue explant culture technique. Im-munohistochemical staining was performed to observe vimentin in fibroblasts. The proliferation of fibroblasts was determined by MTT as-say. Hydroxyproline in the cultural medium was determined by a digestion method. Results:The cells were typical fibroblasts, and the immunohistochemistry staining of vimentin in the cells was positive. The three herb extracts could promote the proliferation of the fibro-blasts at appropriate concentrations in a time-dependent manner. After the 72-hour culture in the medium, 0. 312 5-5. 000 0 mg·ml-1 Aloe vera gel extract, 0. 156 3-2. 500 0 mg·ml-1 Bletilla striata extract and 0. 075 0 mg·ml-1 salvianolate showed significant effects on the proliferation of fibroblasts (P<0. 05 or P<0. 01 vs the blank group). Compared with that in the blank group, the amount of hydroxyproline in the three herb extracts cultural medium was also increased (P<0. 05 or P<0. 01). The effect of salvianolate on the cell proliferation was closely related with time and concentration, and the high concentration group could inhibit the growth of fibroblasts as the extension of time. Conclusion:Aloe vera gel extract, bletilla striata extract and salvianolate can promote the proliferation of fi-broblasts and the production of hydroxyproline, which may contribute to the wound healing.

Objective:To optimize the formula of amlexanox nasal thermosensitive gel spray and establish the quality control meth-od. Methods:Amlexanox nasal thermosensitive gel spray was prepared by a cold dissolving method, and poloxamer 407 (P407) and poloxamer 188 (P188) were used as the carrier materials. Central composite design-response surface methodology was used to optimize the formula with the amount of P407 and P188 as the influencing factors and the gel temperature and the viscosity before gelling as the indices. The content of amlexanox was determined by HPLC. According to Chinese Pharmacopoeia (2010 edition), the other indices of the preparation such as appearance, pH, viscosity, content, total spray times of each bottle and the content of each spray were deter-mined as well. Results:The optimum ratio of P407 and P188 was 17. 0% and 0. 9%,respectively . The average recovery of amlexanox was 98. 8% and RSD was 1. 7%(n=9). The quality of 3 batches of amlexanox nasal thermosensitive gel spray met the related require-ments. Conclusion:The formula and preparation process of amlexanox nasal thermosensitive gel spray are reasonable and feasible with controllable quality, which is worthy of further research.

Objective:To optimize the formula of ropivacaine hydrochloride transdermal gel. Methods:The steady transdermal rate and cumulative transdermal percentage in 24 h of ropivacaine hydrochloride gel were used as the indices, an orthogonal design was applied to select the optimal formula, and Design Expert 8. 0. 5. 0 software was used to analyze the results. Results: The optimal formula con-tained 2% carbomer, 10% propylene-glycol and 5% Azone. The steady transdermal rate of the optimal formula was 0. 6951 mg·h-1 · cm-2 . The cumulative transdermal percentage in 24 h of the optimal formula was 91. 04%, which was 22. 79% higher than that of ropiva-caine hydrochloride solution with the same concentration. Design Expert 8. 0. 5. 0 software could predict the steady transdermal rate and cumulative transdermal percentage in 24 h of the optimal formula. Conclusion: The preparation design is reasonable, and the gel has promising properties, which is suitable for skin local application.

Objective To investigate the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) after cerebral infarction and treatment with curcumin combined with electroacupuncture.Methods Middle cerebral artery occlusion was successfully introduced to simulate cerebral infarction in 60 healthy,male Wistar rats.They were then randomly divided into a control group,an electroacupuncture group,a curcumin group and a curcumin combined with electroacupuncture group (the observation group),each of 15.After the modeling,the four groups were given the planned interventions for 2 weeks,with the control group receiving no intervention.All of the rats were sacrificed after neurobehavioral scoring and immunological staining of brain tissue from the infarction area was used to detect the expression of BDNF and NGF.Results The average Bederson score in the observation group was significantly lower than those of the other groups.Cells expressing BDNF and NGF were significantly more numerous in the observation group than in the other three groups.Conclusion Curcumin combined with electroacupuncture can significantly improve the expression of BDNF and NGF in the infarcted area,and this may play a neuroprotective role.

Objective To investigate the changes of serum and bile from victims attacked by infantile hepatitis syndrome(IHS).Methods The constituents from 42 IHS subjects and 16 controls,including total bilirubin(TB),direct bilirubin(DB),alanine aminotransferase(ALT),glutamyltranspeptidase(?-GT),total bile acid(TBA),interleukin 6(IL-6) and tumor necrosis factor ?(TNF-? )both in bile and serum,were assayed by fully-auto chemistry analyzer and ELISA,respectively.The subjects of IHS were divided into cholestasis group and hepatitis group.Results Of IHS group,the values of serumal TB,DB,ALT,?-GT,TBA,IL-6 and TNF-? were higher than those of control(P_a