Objective To observe the effect of Tongxinluo capsule combined with Jinlidas intervention on vascular endothelial fuction and blood glucose in the patients with Type 2 diabetes. Methods A total of 100 patients with Type 2 diabetes were randomly divided into treatment and control groups with 50 patients each. Both groups were received subcutaneous injection insulin, oral admistration of Simvastatin and Aspirin. And the treatment group were received Tongxinluo capsule and Jinlida sintervention based on the standard treatment. The changes of serum levels of FPG, 2hPG, HbAlc, VEGF, NO, ET-1 were observed before and after treatement. All the patients were treated for 12 weeks. Results After the treatment, the serum of FPG (7.03 ± 2.11 mmol/L vs. 8.09 ± 2.03 mmol/L, t=1.911), 2hPG (9.27 ± 2.03 mmol/L vs. 12.06 ± 3.54 mmol/L, t=1.946), HbAlc (6.98%± 1.58%vs. 8.06% ± 2.13%, t=2.013) were decreasd (P<0.05). Besides, VEGF (684.61 ± 84.37 ng/L vs. 514.28 ± 79.52 ng/L, t=2.136), NO (43.28 ± 6.40 ng/L vs. 34.64 ± 6.70 ng/L, t=2.097) of treatment group were siginificantly higher than the control group (P<0.05), while ET-1 (53.13 ± 7.24μmol/L vs. 60.09 ± 7.18μmol/L, t=2.271) were siginificantly lower (P<0.05). Conclusion Tongxinluo capsule combined with Jinlida sintervention could decreased the level Blood glucose and improve vascular endothelial fuction in the patients with Type 2 diabetes.

The principle and structure of the near-IR laser spectrum noninvasive blood glucose detection system are introduced in the paper. To meet the detection demand of the little DC current signal in the system,the current measure methods are analyzed. The hardware and software structure are discussed in the end.

Objective To explore the related factors about acute stress responses of the military freshmen,then built a path model among self-efficacy,social support,coping style,state-trait anxiety and acute stress responses.Methods 584 students were tested by General Self-efficacy Scale (GSES),Perceived Social Support Scale (PSSS),Coping Styles Questionnaire,State-Trait Anxiety Inventory (STAI) and Acute Stress Response Scale ( ASP S).Results ①The military freshmen in each factor of acute stress response got a lower score,especially the mean score of the changes of cognition was higher (0.21 ±0.21 ),compared with the other factors,and the changes of pathology (0.053 ±0.114) was lower.②There were significant correlations between self-efficacy,social support,positive coping,negative coping,state anxiety,trait anxiety and acute stress responses (P ＜ 0.01 ).③ Negative coping( r=0.130),trait anxiety(r=0.005) and state anxiety(r=0.002) influenced ASR directly,meanwhile,self-efficacy( r =-1.292) influenced ASR through the state anxiety indirectly,self-efficacy ( r=-7.465 ) and social support ( r =-0.294) influenced ASR indirectly through trait anxiety.Conclusion Acute stress responses of the military freshmen were directly influenced by negative coping and state-trait anxiety,and negative coping style was the main reason.

Objective To investigate the influence of different hemostatic way on ovarian function in laparoscopic excision of ovarian cysts. Methods 87 cases with unilateral ovarian cysts by laparoscopic excision were chosen from Jun. 2012 to Jun. 2013 in our hospital, and they were divided into the electrocoagulation group (44 cases) and the suture group (43 cases) according to hemostatic way. The electro-coagulation group were given bipolar coagulation while the suture group were given endoscopic hemostasis. The ovarian hormone levels and blood parameters before and after surgery were compared between the two groups. Results There was no statistically significant differenc in parameters of E2,FSH,LH,P before surgery between the two groups (P>0. 05), and there was no statistically significant differenc before and after surgery in the same group (P>0. 05). Compared with the suture group, there were statistically significant difference in terms of pa-rameters of E2,FSH in the electrocoagulation group(P<0. 05). There was no statistically significant difference in ovary sectional area and PSV between the two groups before surgery (P>0. 05). It was of no statistically significant difference in ovary sectional area between the two groups after surgery (P>0. 05), but it was of statistically significance in PSV (P<0. 05). Conclusion Compared with electrocoagulation, the laparoscopic excision of ovarian cysts with endoscopic hemostasis had less damage to ovarian, and it could protect the ovarian function.

To investigate the inhibitory effects of siRNA expression vector on proto-oncogene Pokemon expression in SW480 cells and to provide experimental basis for further research about the biological function of Pokemon.siRNA expression vectors were constructed to express a short hairpin RNA against Pokemon.The recombinants were transfected into SW480 cells with liposome.Cellular morphology and transfection efficiency were observed.The expression of Pokemon were checked by real-time fluorescence quantitative PCR and Western blot.MTT assay was used to detect the effect of siRNA on the growth of SW480 cells and Fluorescence Activated Cell Sorter was applied to study cell apoptosis of SW480 cells.The transfection efficiency was about 36% and the cellular morphology changed greatly at 48h after transfection.siRNA expression vectors could specifically reduce the expression of Pokemon mRNA and protein in SW480 cells.Compared with negative control,the inhibition ratio of Pokemon mRNA expression was 34.2% and 67.7%,in 24th hour and 48th hour,the inhibition ratio of Pokemon protein was 48.3% and 73.6%,in 48th and 72th hour,respectively.Transfection with Pokemon siRNA expression vectors could inhibit cell growth and induce cell apoptosis of SW480 cells.siRNA expression vectors were successfully established that could effectively inhibit the expression of Pokemon in SW480 cells.Pokemon gene silencing could decrease growth and increase apoptosis of SW480 cells.

Programmed death-ligand 1 (PD-L1 ),which is highly expressed in NSCLC,can be divided into two categories:membrane PD-L1 and soluble PD-L1 .PD-L1 participates in tumor immune escape through combining with its receptor.PD-L1 immune checkpoint inhibitors have entered the phase Ⅰ studies and showed a good application prospect.It is also found that the sensitivity of PD-L1 immune checkpoint inhibitors is strongly associated with the expression of PD-L1 in tumor.Therefore,PD-L1 can be used as a biomarker to pre-dict its curative effect.

This article was aimed to study the therapeutic material basis of Xiong-Shao (XS) decoction on hepatic fi-brosis (HF), and screen effective parts from XS decoction for protecting liver, reducing enzyme activity and oxidative damage. Male wistar rats were randomly divided into the normal group, model group, Fu-Zheng Hua-Y u (FZHY) group, XS group, polysaccharide group, total alkaloids group and the total glycosides group. HF rat model was estab-lished with the intraperitoneal injection of DMN. After modeling, FZHY solution (0.105 g·mL-1), XS decoction (1.610 g·mL-1), crude polysaccharides extract of XS decoction (35.420 mg·mL-1), total glycosides extract solution (25.725 mg·mL-1), and total alkaloids extract of XS decoction (0.196 mg·mL-1) were administered to corresponding treatment group by gavage once a day for 4 weeks, respectively. Rats of the normal group and model group were given equiva-lent normal saline by gavage once a day for 4 weeks. After 4-week drug administration, rats were killed to remove the liver. Automatic biochemical analyzer was used in the detection of serum parameters of liver function, including ALT, AST, TIBL and ALB. Serum SOD activity was detected by xanthine oxidase. GSH-PX activity was tested by DTNB reduction. Serum contents of MDA were measured by TBA. Pathological changes of the liver were observed with HE staining and masson staining. The results showed that compared with the model group, there was no signifi-cant differences between the total alkaloids group and the model group, but levels of serum ALT, AST and TBIL of other treatment groups were significantly decreased, and the serum ALB level was significantly elevated (P< 0.05 or P < 0.01). Compared with the model group, levels of serum SOD and GSH-PX of the FZHY group, XS group and total alkaloids group were significantly elevated (P < 0.01), and level of serum MDA was significantly reduced (P <0.05 or P< 0.01). Comparison among the polysaccharides group, total glycosides group, and model group showed no significant differences. It was concluded that crude polysaccharide and total glycosides fractions were effective parts of XS decoction for protecting liver and reducing enzyme activity. And total alkaloids fraction was the effective part for reducing oxidative damage.

Objective To determine the prevalence of dental anxiety in children patients before tooth extraction.Methods　A modified Children's Fear Survey Schedule-Dental Subscale (CFSS-DS) and Venham's clinical ratings of anxiety and cooperative behavior were used in 150 children patients ranging in age from 5 to 12 years olds before tooth extraction. The former scale was answered by parents on behalf of their children, and the latter ratings was assessed by the author. Results　CFSS-DS scores and clinical ratings of behavior of the children were significantly higher in the children whose oral hygiene condition, dental experience, tooth extraction experience, spirit status on that day and whose mother's educational background were bad or low. A step regression analysis showed that oral hygiene condition had most significant interrelationship with both of the two scales（P＜0.01）. The correlation of the two scales was high (r=0.67).Conclusion　Children’s oral hygiene condition, dental experience, tooth extraction experience, spirit status on that day and their mother’s educational background are closely related to the dental anxiety level. Oral hygiene condition is the most important predictor of anxiety level before extraction and clinical ratings of behavior during extraction.

BACKGROUND: Studies have indicated that the abnormal expression of TACC3 is closely related to the occurrence and development of many kinds of tumors, and the expression of TACC3 is up-regulated in these tumors. Therefore, in vitro specific inhibition of TACC3 expression may become an important target for the treatment or intervention of tumor growth.OBJECTIVE: To investigate the mechanism by which TACC3 gene expression regulates cell proliferation and apoptosis in oral squamous cell carcinoma.METHODS: CD133+CD44+ oral squamous cell carcinoma cells were sorted from human oral squamous cell carcinoma cell line Cal-27 by immunomagnetic beads. In experimental group, the shRNA sequence of TACC3 was designed and synthesized, which was then trasnfected into CD133+CD44+ oral cancer stem cells by LipofectamineTM 2000. Empty vector-trasnfected (negative control) and untransfected cells were used as callsed. Forty-eight hours after the transfection, effects of TACC3 gene silencing on proliferation and apoptosis in vitro in CD133+CD44+ oral squamous cell carcinoma were detected by MTT, clone formation test, and TUNEL assay. Western blot assay was used to detect the effect of TACC3 gene silencing on Ki67, Bax and Bcl-2 protein expression in CD133+CD44+ oral squamous cell carcinoma.RESULTS AND CONCLUSION: (1) Cell proliferation. The proliferation rate and expression level of Ki67 were significantly lower in the experimental group than the negative control and untransfected groups (P < 0.05). (2) Clone formation. The clone formation ability in the experimental group was significantly lower than that in the negative control and untransfected groups (P < 0.05). (3) Cell apoptosis. TACC3 gene silencing caused an obvious decrease in Bcl-2 protein expression and a significant increase in Bax protein expression. These findings further confirmed that specific interference of TACC3 gene expression could inhibit the proliferation of CD133+CD44+ cells and promote the apoptosis.

0.05).The activities degrees of ankle joint also had no statistical difference.[Conclusion]According to the extent of the displacement of Cotton's fracture,we should make the right operative order and the incision,thus we can get results with anatomic reduction and shorten the operative time,which can help the patients make early exercises and get satisfactory effect.