Random clustering was conducted among a total of 1068 residents aged 18 years or older at a certain Shanghai community.And they completed a questionnaire survey.Logistic data processing and model building were performed.The respondents were willing to go to a community health center for the following reasons:close proximity (88.4％),excellent service attitudes (40.4％) and low drug prices (37.8％).No statistically significant differences existed in terms of first choices of community health service,occupation,monthly income and-walking distance (P ＞ 0.05).However,statistically significant differences existed in education level,age and chronic diseases (P ＜ 0.05).

Calmodulin,as an important signaling regulatory protein,widely exists in eukaryotic cells and participates in a variety of physiological activities.Alzheimer's disease (AD) is a typical neurodegenerative diseasemostly affecting the old people.Senile plaques (SP) caused by amyloid protein deposition and neurofibrillary tangles (NTF) caused by over-phosphorylation of Tau protein in the brain are the two major neuropathological hallmarks of AD.Neuronal apoptosis induced by dysregulation of calcium homeostasis also plays a significant role during this pathological process.Recent studies have found that calcium and its downstream receptor,calmodulin,are closely associated with the pathogenesis and progress of Alzheimer's disease.This article reviews the current development and the latest research progress on the effects of calmodulin on β-amyloid protein generation and deposition,neurofibrillary tangle formation,synaptic plasticity in AD around the world,comprehensively discusses the role of calmodulin in the pathogenesis of Alzheimer's disease.

Animals ; Calpain ; Humans ; Nervous System Diseases ; chemically induced ; Organophosphate Poisoning

Working time of normal setting impression materials was 71 (hand mixing,30 s),66 (Alghamix,20 s) and 53 (Algimax-Ⅱ,8 s) seconds respectively.Working time of fast setting impression materials was 56 (hand mixing,30 s) and 51 (Alghamix 20 s) seconds respectively.Setting time of normal setting impression materials was 163 (hand mixing,30 s),160(Alghamix 20 s) and 124(Algimax-Ⅱ,8 s)seconds respectively.Setting time of fast setting impression materials were 131 (hand mixing,30 s) and121 (Alghamix 20 s) seconds.The working and setting time of normal setting impression materials mixed by hand mixing(30 s) and auto-mixing (Alghamix 20 s;Algimax-Ⅱ,8 s) and fast setting impression materials mixed by hand mixing(30 s) and with auto-mixing using Alghamix (20 s) may satisfly clinical requirement.

· AIM: To observe the effects of PTEN and the tumor suppressor gene in proliferative LECs of the rabbit.·METHODS:Forty-two white rabbits were randomly divided into test group (36 rabbits) and control group (6rabbits). The transparent lenses of treated rabbits were operated with extra capsular cortex extraction, and the controls were kept untouched. The rabbits were sacririced at 1d, 3d, 1wk, 2wK, 1mo and 2mo after surgery.Immunohistochemistry was applied to detect proliferative cellular nuclei antigen (PCNA) as well as hybridization in situ and reverse-transcriptase polymerase chain reactions (RT-PCR) were applied to detect phosphatase as well as tensin homology deleted on chromosome 10(PTEN) mRNA in lens equartor.·RESULTS:PCNA expression increased significantly to a high level at 1wk, began to reduce 2wk later, and recovered to the normal level 1 or 2mo after the surgery.PTEN mRNA expressed positively in normal rabbit LECs.The relative PTEN mRNA contents reduced greatly at 1dafter operation and remained low level at 3d. It began to increase slightly at 1wk, kept rising at 2wk and regained the normal expression after 1 or 2mo. There was inverse correlation between the PTEN mRNA and PCNA expression.·CONCLUSION: PTEN mRNA expresses positively in normal rabbit LECs plasma. PTEN participates in LECs proliferation and correlates with lens proliferative conditions.

The flip classroom,as a new teaching form,has innovated the traditional teaching model.Although the flip classroom has its superiority,it will encounterchallenges in practice.This paper firstly analyzed the feasibility and necessity of medical ethics education based on the flip classroom.Then,it introduced the teaching model of medical ethics flip classroom based on three aspects:the basic pattern design,learning resources design and teaching process design.Finally,it discussed the application and effects of the flip classroom on the teaching of medical ethics.

Objective To study nuclear factor E-2 related factor 2(NrF-2) and heme oxygenase -1(Ho-1) expression and to explore its clinical significance.Methods From February 2016 to June 2016, 176 cases human gastric tissue paraffin blocks selected in department of pathology in Hangzhou Cancer Hospital, including 78 cases of gastric cancer, 78 cases of normal tissue were analyzed.And the expressions of NrF-2 and Ho-1 were detected by immunohistochemistry in two groups of human gastric tissue.Results The levels of NrF-2 and Ho-1 in gastric cancer group were(0.752 ± 0.098),(0.862 ±0.081) were significantly higher than those in normal group(0.381 ±0.068),(0.412 ±0.083);NrF-2, the positive expression rate of Ho-1 in gastric wall invasion degree of T3 +T4 with lymph node metastasis, NTM stage III and IV gastric cancer tissues were significantly increased.The differences were statistically significant ( P <0.05 ) .Conclusion NrF-2 and Ho-1 play the important role in the development of gastric cancer and NrF-2, the positive expression rate of Ho-1 in the gastric carcinoma and gastric wall invasion, lymph node metastasis and NTM staging are closely related.The clinical diagnosis if the combined detection of NrF-2 and Ho-1, is conducive to the early diagnosis of gastric cancer and the prognosis of the disease.

To design a sphygmomanometer detecting worktable to prevent the leaked mercury from flowing or doing harm to the staff after volatilization. The worktable was composed of a body, an exhaust hood, a flow-guided groove, a base, a flow-guided hole, a collecting bin, a ventilating fan and etc. A filter screen at the ventilating fan was equipped with the sulphur powder to react with the mercury vapor in the air. The worktable could reduce mercury pollution and its damage to the staff. The worktable facilitates the maintenance and detection of the sphygmomanometer, and thus is worthy popularizing clinically.

Objective:To improve the method for isolating platelet-rich plasma (PRP). Methods:Whole blood was collected from 8 healthy donors and then PRP was separated by both the tube method and the syringe method respectively. Samples were activated to get serum rich-in growth factors (SRGF).Platelets in the SRGF were counted and the level of TGF-?1 was assayed by enzyme-linked immunosorbent assay (ELISA).Results:The end product of syringe method has both a higher platelet count in PRP (P=0.003) and a higher level of TGF-?1 in SRGF(P=0.041) than that of tube method.Conclusion:The syringe method is an effective method in preparation of PRP.

Objective To study the expression and significance of fatty acid synthase ligand ( sFas ) and soluble fatty acid synthase receptor (sFasL) on the neoadjuvant chemotherapy of breast cancer.Methods 84 cases of patients with breast cancer neoadjuvant chemotherapy were recorded from January 2015 to January 2016 in our hospital as breast cancer group,another 84 cases of healthy subjects as control group,the two groups were measured sFas,the positive rate of sFasL.At the same time,respectively before and after neoadjuvant chemotherapy, detection of patients serum sFas, sFasL levels and breast cancer.According to the clinical curative effect were divided into effective group and ineffective group , compared two groups of serum sFas, sFasL levels.Results Breast cancer group called sFas and sFasL positive rate was 58.33% (49/84) and 100.00%(84/84),were significantly higher than that of control group 38.10%(32/84) and 0.00% (0/84);The level of sFas in patients with neoadjuvant chemotherapy was significantly lower than that of neoadjuvant chemotherapy, and the level of sFas in neoadjuvant chemotherapy group was significantly higher than that in ineffective group, the difference was statistically significant (P<0.05).Conclusion In the course of neoadjuvant chemotherapy for breast cancer,the efficacy of neoadjuvant chemotherapy can be effectively detected by detecting the level of serum sFas in patients with breast cancer , while sFasL has no significant change in the neoadjuvant chemotherapy of breast cancer.