Objective]Explore the Neijing·Lingshu and channels and col aterals theory of traditional Chinese medicine on root end, the meaning of the appearance substance, the development and application.[Methods]Through consulting relevant literature, the paper expounds root end, appearance substance in channels and col aterals theory, the definition and connection, and the root knot, appearance substance and qi and blood, specific hole, gas street, universal and systematic review of clinical application, etc. [Results]Root end is similar but not identical and appearance substance theory, root end heavy concrete location, up and down on both ends of the appearance substance weight area. Qi by no out, a way to root, slip, note, and five acupoints, the head of the parts to the auction, offering acupuncture point, back-shu point, the acupuncture point, etc., in accordance with the gas tract, universal distribution position. Come a long way to Selecting distant points and root-pass-knot match point, provide theoretical basis for clinical application. [Conclusions]Root end, appearance substance theory is a supplement to the theory of meridians and improvement of the clinical syndrome differentiation, finding out the formula and specific point one of the clinical application provides the theoretical methods, the application method is an extension of the concept on also to expand scope provided theoretical guidance for the treatment of disease. The disease diagnosis and treatment have important significance.

Chimeric antigen receptor natural killer cell (CAR-NK) is a new promising immunotherapy. NK cell can derive from peripheral blood cells, cord blood cells, NK-92 cell line and iPSC cells. CAR-NK cells can recognize target antigens non-specifically and are not restricted by human leukocyte antigens. Allogeneic infusion of CAR-NK cells does not cause graft-versus-host disease. It has a strong effect on primary cells of leukemia, lymphoma and multiple myeloma, thus making animal models have strong anti-tumor effect. Therefore, as a "off-the-shelf" product, CAR-NK cells have a wide range of clinical applications. In this paper, the construction of CAR-NK vector and its preliminary clinical application in hematological tumors and the challenges were reviewed.

The research of induced phripotent stem(ips)cells is a hotspot in the area of life sciences at present.Currently,the research of ips cells has focused on cells'induction methods,source and differentiation.At the same time,the research of ips cells'application has also made some achievements.With the extensive research of application,ips cells will have an inestimable effect on the future of biomedical development.This article will give an reviews the latest application and future research trends of ips cells.

Objective To investigate the effects of dexmedetomidine preconditioning on nitric oxide (NO)/endothelin (ET)-1 imbalance and remote lung injury induced by lower limb ischemia-reperfusion (LIR). Methods Sixty patients who scheduled for unilateral lower extremity surgery matched American Society of Anesthesiologists (ASA) Ⅰ-Ⅱ, were randomized into two groups:control group (R group, n=30) and dexmedetomidine preconditioning group (PD group, n=30). Lumbar plexus combined with sciatic nerve block was performed guided by a nerve stimulator in both groups. In group PD, dexmedetomidine intravenous infusion was started at a dose of 0.125 mL/kg (4 mg/L) for 10 minutes before using tourniquet, whereas group R received an equivalent volume of normal saline. Artery blood gas analysis, respiratory index and oxygenation index were measured, and NO, ET-1, interleukin-8 (IL-8) and malondialdehyde (MDA) concentrations were determined from plasma samples 10 minutes before tourniquet inflation (T0), 15 minutes (T1), 2 h (T2), 6 h (T3) and 24 h (T4) after tourniquet deflation. Results Compared with T0, RI was higher at T3 in group R and OI was lower at T2-4 (P＜0.01). There were no significant differences in RI and OI of group PD between different time points (P＞0.05). In R and PD groups, ET-1, IL-8 and MDA concentrations were increased, while NO level and NO/ET-1 ratio were significantly decreased after tourniquet deflation (P＜0.05). Compared with group R, RI was lower and OI was higher at T3 in group PD (P＜0.01). The levels of ET-1, IL-8, MDA, NO and NO/ET-1 ratio were significantly different after tourniquet deflation between group PD and group R (P＜0.05). In group R, there was positive correlation between ET-1 and IL-8 levels with RI, and negative correlation between ET-1 and IL-8 levels with OI (P＜0.01). There was positive correlation between NO level, NO/ET-1 ratio and RI level (P＜0.01). Conclusion Lung function impairment induced by tourniquet application could be attenuated by dexmedetomidine preconditioning based on protecting endothelial cells and inhibiting lipid peroxidation.

Communicable Disease Control ; Humans ; Research

Objective The aim of this article is to study the clinical features, image, electroencephalogram and gene mutation of mitochondrial encephalomyopathy with MELAS and to improve understanding of the disease. Methods Clinical data was collected from 12 MELAS cases. Their clinical symptoms, head imageological result,electroencephalo-gram and gene mutation were analyzed. Results The average onset age was 28 years old. The main manifestations of 12 patients were epilepsy(66.7%), stroke-like episodes(50.0%), mental behavious disorder and decrease of perceive (41.7%),diabetes or abnormal glucose tolerance(41.7%), hearing impairment(41.7%). The imaging results showed long T1, long T2 and high signals on DWI. The nidus involved occipital lobe, temporal lobe, parietal lobe most. Five of six cases undergoing MRS imaging had large lactate peak at 1.33 ppm. There were 9 cases carrying mtDNA A3243G muta-tion. Conclusion The clinical presentation of MELAS is highly variable without any obvious specificity on MRI and EEG. Large lactate peak on MRS may indicate the disease. Most patients carry A3243G mutation.

Objectives To study the effects of effective constituent of Cortex Moutan-Paeonol and Paeoniflorin on myocardial ischemia and myocardial infarction in experimental canines.Methods The coronary artery ligation was employed to establish the model of canines with acute myocardial ischemia and myocardial infarction.The extent of myocardial ischemia was detected by epicardial-electrocardiogram and the extent of infarction was determined by N-BT staining method.Results The adminstration of Paeonol and Paeoniflorin reduced the myocardial ischemia level(P

ObjectiveTo observe the different mode of administration of lidocaine on hemodynamic effects during self-retaining laryngoscope.Methods75 adult patients,underwent selective operations of removal and biopsy of the vocal polype,epiglottis or laryngeal tumor via self-retaining laryngoscope under general anesthesia,were randomly divided into three groups,with 25 cases for each.Group Ⅰ was the control group; Group Ⅱ was the throat spraying group;Group Ⅲ was the intravenous drug group.The incidence of irritating cough and the changes of mean arterial pressure(MAP) and hear rate(HR) were observed during operations.ResultsThe incidence of irritating cough in group Ⅱ was lower than in group Ⅰ and group Ⅲ ( all P ＜ 0.05 ).MAP,HR in group Ⅱ was also lower than that in group Ⅰ and Ⅲ during extubation( all P ＜0.05).However there were no significant differences on MAP,HR between group Ⅰ and group Ⅲ during extubation( all P ＞ 0.05 ).ConclusionLidocaine spraying on larynx could alleviate irritating cough and had significant preventive effect on cardiovascular response,and the effect in this method was superior to intravenous injection.

Aged ; Atropine ; Electrocardiography ; Female ; Heart Rate ; drug effects ; Humans ; Male ; Muscarinic Antagonists ; pharmacology ; therapeutic use ; Preoperative Care ; methods ; Quinuclidines ; pharmacology ; therapeutic use

OBJECTIVETo perform a transcriptomics study in differential genes after Xianglian External Lotion (XEL) induced the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole.

METHODSBroth microdilution antifungal susceptibility test was used to detect minimal inhibitory concentration (MIC) of drug-resistant Candida albicans strains induced by XEL. Transcriptome sequencing (RNA-Seq) was used to determine and compare the transcription of primary drug-resistant Candida aIbicans strains and sensitive strains induced by XEL. High expressed genes and signaling pathways strains were analyzed by gene ontology (GO) method.

RESULTSXEL could induce drug-resistant strains of the 6th generations to recover sensitivity. Transcriptome sequencing showed that, as compared with primary drug-resistant strains, there were 165 genes with up-regulated RPKM index and 144 genes with down-regulated RPKM index after XEL induction. GO analyses found that all genes were mainly classified as GO:0015903 (fluconazole transport).

CONCLUSIONSXEL could induce the recovery of drug-resistant Candida albicans strains sensitive to Fluconazole. By analyzing transcriptomes, authors speculated that XEL could recover strain sensitivity to fluconazole by opening fluconazole transport pathway.

Antifungal Agents ; pharmacology ; therapeutic use ; Candida ; Candida albicans ; drug effects ; genetics ; Candidiasis ; drug therapy ; Drug Resistance, Fungal ; drug effects ; genetics ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Fluconazole ; pharmacology ; Microbial Sensitivity Tests