Objective:To observe the inhibitory effects of survivin-specific siRNA on human lung cancer cell lines with different P53 statuses,and to analyse the relationship between P53 and survivin in vitro.Methods:A549(wild-type P53)and H1299(P53 absent)cells were transfected with chemosynthetic survivin siRNA.The effect of siRNA on cell proliferation was analysed by MTT assay.The changes of cell cycle and apoptosis were examined by flow cytometry.The expression of survivin mRNA was detected by RT-PCR.The protein expression of survivin,P53,P21 and PARP was examined by Western blotting.Results:Endogenous survivin level in A549 cells was lower than that in H1299 cells.The mRNA and protein expression of survivin was significantly lower in siRNA group than in blank control group and non-silencing siRNA group(P

OBJECTIVE:To discuss the ways of implementing national essential drug policy in order to actualize its function of restriction and orientation.METHODS:The background of implementing national essential drug policy in China was reviewed.The problems in the course of its implementation and the reasons for weak implementation were analyzed,and the detailed steps and measures for strengthened implementation of the policy were put forward.RESULTS&CONCLU-SIONS:It is imperative to explore,analyze and solve the problems in the implementation of national essential drug policy.Powerful implementation of the policy is one of the effective routes to solve the problems such as high drug price,difficult consultation and irrational drug use.

Objective: To explore the changes of nuclear factor-κB (NF-κB) activity, TNF-α, IL-1β and IL-10 contents in the lung tissue of rabbit with seawater drowning-induced acute lung injury (SWD-ALI) and the effects of dexamethasone on the changes. Methods: Forty-two New Zealand white rabbits were randomly allocated to control group (C group, n=18) , seawater drowning group (S group, n=12) and dexamethasone treatment group (D group, n= 12). The drowning model was established by instilling seawater (2 ml/kg body weight) into the endotracheal catheter of animals, then the rabbits received arterial injection of dexamethasone (D group, 1 mg/kg body weight) or 2 ml normal saline (S group). Blood gas analysis was done at predefined time points. The lung wet to dry weight ratio (W/D) and lung permeability index (LPI) were calculated. The activity of NF-κB was analyzed by non-radioactive electrophoretic mobility shift assay (EMSA). The contents of TNF-α, IL-1β and IL-10 were detected by ELISA. Meanwhile, the pathology changes of lung tissues were detected by H-E staining, and the semi-quantitative lung pathologic scores (LPS) was also calculated. Results: The lung of rabbits in S group was obviously enlarged and had more severe edema and congestion compared with C group; furthermore, histopathologic findings indicated inflammatory cell infiltration and other pathologic signs of ALI; the oxygenation index (PaO 2/FiO2) reached the bottom at 0.5 h and did not elevate to more than 300 mmHg(1 mmHg=0.133 kPa) until 6 h. The largest W/D ratio appeared at 3 h after seawater drowning, and the highest LPI and LPS appeared at 6 h; and NF-κB activity and the contents of TNF-α, IL-1β and IL-10 in lung tissues were significantly higher in S group than in C group(P<0.05, P<0.01). The pathological changes of lung in the rabbits of D group were improved compared with S group but worse than those of group C. The W/D ratio, lung permeability index, and lung pathological score in D group were lower than those of the S group; and the oxygenation index was greatly improved 6 h after seawater drowning. NF-κB activity and the contents of TNF-α, IL-1β and IL-10 in lung tissues were significantly lower than those of the S group(P<0.05, P<0.01). Conclusion: Dexamethasone can inhibit the activity of NF-κB and the expression of TNF-α, IL-1β and IL-10 in the lung tissue of rabbits with SWD-ALI and relieve the inflammatory responses and pathological changes.

OBJECTIVE:To optimize the approach and methods for the selection of national essential drugs and to improve national essential drug system.METHODS:The concept of national essential drugs and the principle of selecting national essential drugs and the problems within were expounded,and the rationale and methods of pharmacoeconomics were adopted to analyze its significance and role in selecting national essential drugs.RESULTS & CONCLUSION:Simplifying National Essential Drug List can facilitate peoples’ access to essential drugs.

OBJECTIVE:To enhance the competitive edge of Chinese pharmaceutical enterprises.METHODS:Patents problems confronted by Chinese pharmaceutical enterprises were analyzed in terms of the research and development and patent protection of the original new drugs,the administrative protection and patent protection of drugs,patent documentation in?formation,patent strategy and patent talents,etc.RESULTS&CONCLUSION:Chinese pharmaceutical enterprises should highlight the patent problems and apply patent strategy to achieve competitive advantage.

OBJECTIVE:To analyze the current status of counterfeit in China and indicate the difficultie of identification.METHODS:The main characteristics of counterfeit drugs were discussed by representative cases.RESULTS & CONCLUSION:It is suggested that the department of the drug administrave should establish a more scientific,reasonable standardization procedure of conterfeit drugs identification aiming directly at the characteristics of counterfeit drugs,which was more sufficient,intensive and practicable legal evidence for defining,fighting and preventing counterfeit drugs.

OBJECTIVE:To provide reference for the establishment of coordinative mechanism among different interest groups during the implementation of essential drug system. METHODS:Based on game theory,game behaviors of various interest groups were studied with public medical institution as study core. Behavior relationships among various interest groups were ensured to find out equilibrium point of interests. RESULTS & CONCLUSIONS:In the implementation process of National Essential Drug System,great importance should be attached to the study on game phenomena and behavior among various interest groups to make sure implementation of National Essential Drug System.

The rational drug use of elderly patients with chronic diseases had many problems which seriously affect the live and life safety of patients.To convey the importance of rational drug use from the perspective of humanistic care for patients can help to improve the treatment compliance of patients,promote the ability of self-management and build a harmonious doctor-patient relationship.Medical staff should pay attention to enhance the care for the elderly patients,optimize drug treatment programs,improve diversified social support systems and use various medium and forms to propaganda the knowledge of rational drug use.

Drug Combinations ; Drug Design ; Drugs, Chinese Herbal ; pharmacology ; Drugs, Investigational ; Humans

The incidence of spinal cord injury is becoming higher and higher, of which more than half are cervical spinal injury. The main cause of death in high cervical spinal injury is respiratory function failure. The patients who survived must rely on ventilators to sustain life. In view of the shortcomings of ventilators, many researchers tried to use diaphragm pacing technology instead of ventilator to reconstruct the function of respiratory. This article introduced the application of diaphragm pacing technology in patients with cervical spinal injury.