Acute-on-chronic liver failure (ACLF) is a critical disease in clinical practice and has a mortality rate as high as 80％.At present,there are still no ideal therapeutic measures for ACLF.Stem cell transplantation has unique advantages in the treatment of ACLF;however,there are still some issues regarding the research on stem cell transplantation,which limit its clinical application.This article elaborates on conventional treatment methods,source of stem cells,transplantation approach,and related issues,in order to provide help for further studies.

AIM: To investigate the antimicrobial activity of linezolid,vancomycin and other antibacterials against 111 methicillin resistant staphylococcus aureus.METHODS: Broth dilution method was used to determine the minimum inhibitory concentrations(MICs) of linezolid,vancomycin and other antibacterials against 111 methicillin resistant staphylococcus aureus identified by cefoxitin.RESULTS: The resistant rates of 111 MRSA to ?-lactam,erythromycin,aminoglycosides(netilmicin and gentamycin),fluoroquinolones(gatifloxacin,moxifloxacin and levofloxacin) and chloromycin were 100%,92.8%,99.1%,91.9%-99.1%,3.6%.All 111 MRSA were sensitive to linezolid and vancomycin,and MIC50 and MIC90 of vancomycin were 0.5 and 1 ?g/mL,MIC50 and MIC90 of linezolid were 2 ?g/mL.CONCLUSION: 111 MRSA were resistant to most antibacterials including ?-lactam,erythromycin,aminoglycosides and fluoroquinolones.Linezolid and vancomycin have strong antimicrobial activity against MRSA.

Objective To investigate the clinical value of shortening double-J stent indwelling time in the treatment of ureteral stone-street complications after flexible ureteroscope lithotripsy . Methods Clinical data of 38 cases of ureteral stone-street complications following flexible ureteroscope lithotripsy from January 2012 to December 2013 in our hospital were retrospectively analyzed.The patients were divided into Group A (extubating the double-J stent two weeks after the surgery ) and Group B (retaining the double-J stent) with 19 cases in each group .The stone clearance results were compared . Results All the urinary calculus in Group A had been removed successfully , including 2 cases of renal colic , 3 cases of irritative symptoms of bladder and 3 cases of gross hematuria.Hospital cost was (766.5 ±153.7) yuan.Urinary calculus were successfully removed in 13 cases in Group B, including 19 cases of gross hematuria and 17 cases of irritative symptoms of bladder.No renal colic occurred.The hospital cost was (1251.2 ± 155.6) yuan.Compared with Group B , Group A has higher success rate of stone clearance , lower hospital cost and lower rate of bladder irritative symptoms and gross hematuria (P<0.05).There were no significant differences between the two groups in the occurrence rate of renal colic (P>0.05). Conclusions Removing the double-J stent 2 weeks after flexible ureteroscope lithotripsy resulted in higher stone clearance rate and less complications compared with retaining the double -J stent.It can reduce the occurrence of irritative symptoms bladder and gross hematuria .

Wuyi is one of the Chinese medicine recorded in Shennong's Herbal Classic and many other herbal books during the long history period. Up to now, Wuyi is still an important medicine used for insecticidal and removing food retention. Recent researches indicated that Wuyi also had the function of anti-malaria and treating herpetic simplex keratitis. Therefore, Wuyi had notable value on clinic and development of new medicine. The herbalogical textual research on Wuyi was conducted through investigation of the literature of materia medica during the long historical period from Han Dynasty. It was demonstrated by this work that the original plants of Wuyi include Ulmus macrocarpa Hance and Hemiptelea davidii (Hance) Planch, in which, U. macrocarpa was the quality specie. U. pumila was the false specie which cause confusing. The medicine Wuyi was the processed product made from the nutlet of the plants through fermentation with other additive agents.
China ; Drugs, Chinese Herbal ; analysis ; Herbals as Topic ; history ; History, Ancient ; Plants, Medicinal ; anatomy & histology ; chemistry ; classification

The achievement of Qimin Yaoshu in the area of herbalogical textual research was illustrated in this paper. Qimin Yaoshu quoted and keeped many contents from ancient agriculture books related in herbalogical literature. It also recorded the information of lots of tropical and subtropical plants. The author bringed forwarded many experiences indistinguishing plants and clarified some long last confused or wrong opinions. The studying philosophy and methods of the authors gave us much enlightenment in our herbalogical textual research. It should be taken into account when the herbalogical textual research was carried out.
China ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Herbals as Topic ; history ; History, Ancient ; Medicine in Literature ; Plants, Medicinal ; anatomy & histology ; chemistry ; classification

The achievement of Nongzheng Quanshu in area of herhalogical textual research was illustrated in this paper. Nongzheng Quanshu recorded the information of 159 kinds of cultivated plants and 473 kinds of wild plants. It also quoted many contents from lots of ancient agriculture hooks related in herbalogical literature. Many agriculture researchers were encouraged to have done much work in area of herhalogical textual research It also included the materia medica hook Jiuhuang Bencao and Yecaipu therein and promoted their delivery.
Books ; history ; China ; History, Ancient ; Materia Medica ; history ; Medicine in Literature ; Plants, Medicinal ; chemistry ; classification

Working time of normal setting impression materials was 71 (hand mixing,30 s),66 (Alghamix,20 s) and 53 (Algimax-Ⅱ,8 s) seconds respectively.Working time of fast setting impression materials was 56 (hand mixing,30 s) and 51 (Alghamix 20 s) seconds respectively.Setting time of normal setting impression materials was 163 (hand mixing,30 s),160(Alghamix 20 s) and 124(Algimax-Ⅱ,8 s)seconds respectively.Setting time of fast setting impression materials were 131 (hand mixing,30 s) and121 (Alghamix 20 s) seconds.The working and setting time of normal setting impression materials mixed by hand mixing(30 s) and auto-mixing (Alghamix 20 s;Algimax-Ⅱ,8 s) and fast setting impression materials mixed by hand mixing(30 s) and with auto-mixing using Alghamix (20 s) may satisfly clinical requirement.

This article was aimed to study the therapeutic material basis of Xiong-Shao (XS) decoction on hepatic fi-brosis (HF), and screen effective parts from XS decoction for protecting liver, reducing enzyme activity and oxidative damage. Male wistar rats were randomly divided into the normal group, model group, Fu-Zheng Hua-Y u (FZHY) group, XS group, polysaccharide group, total alkaloids group and the total glycosides group. HF rat model was estab-lished with the intraperitoneal injection of DMN. After modeling, FZHY solution (0.105 g·mL-1), XS decoction (1.610 g·mL-1), crude polysaccharides extract of XS decoction (35.420 mg·mL-1), total glycosides extract solution (25.725 mg·mL-1), and total alkaloids extract of XS decoction (0.196 mg·mL-1) were administered to corresponding treatment group by gavage once a day for 4 weeks, respectively. Rats of the normal group and model group were given equiva-lent normal saline by gavage once a day for 4 weeks. After 4-week drug administration, rats were killed to remove the liver. Automatic biochemical analyzer was used in the detection of serum parameters of liver function, including ALT, AST, TIBL and ALB. Serum SOD activity was detected by xanthine oxidase. GSH-PX activity was tested by DTNB reduction. Serum contents of MDA were measured by TBA. Pathological changes of the liver were observed with HE staining and masson staining. The results showed that compared with the model group, there was no signifi-cant differences between the total alkaloids group and the model group, but levels of serum ALT, AST and TBIL of other treatment groups were significantly decreased, and the serum ALB level was significantly elevated (P< 0.05 or P < 0.01). Compared with the model group, levels of serum SOD and GSH-PX of the FZHY group, XS group and total alkaloids group were significantly elevated (P < 0.01), and level of serum MDA was significantly reduced (P <0.05 or P< 0.01). Comparison among the polysaccharides group, total glycosides group, and model group showed no significant differences. It was concluded that crude polysaccharide and total glycosides fractions were effective parts of XS decoction for protecting liver and reducing enzyme activity. And total alkaloids fraction was the effective part for reducing oxidative damage.

AIM:To retrospectively analyze the lipid metabolism disturbance and subacute inflammation within the microenvironment of follicular fluid between Chinese polycystic ovarian syndrome ( PCOS ) patients and the controls . METHODS:Serum lipid indexes , steroid hormone levels , and inflammatory cell counts were analyzed .The inflammatory cytokine and apolipoprotein levels were detected in the serum and follicular fluid .The mRNA expression of apolipoproteins and cytokines in the granulose cells was determined by real-time PCR.RESULTS: PCOS patients showed typical obesity accompanied with hyperlipidemia and hyperandrogenemia .Significantly elevated inflammatory cell number and cytokine lev-els were detected in both serum and follicular fluid .The mRNA expression levels of the inflammatory cytokines and apoli-poproteins in the granulose cells from the PCOS patients were higher than those from the controls .CONCLUSION:Elevat-ed apolipoproteins reflect systematic hyperlipidemia in the follicular fluid .Serum lipids and cytokines penetrate follicle-ser-um barrier and get into follicle fluid .Meanwhile , increased intake of apolipoproteins or elevated synthesis of cytokines ( IL-1, IL-6 and TNF-α) by granulose cells could also be crucial to stabilize microenvironment of follicular fluid .Oocyte and subsequent embryos are sensitive to the originaal follicular environment .The lipid metabolism disturbance , inflammatory cell infiltration and hyperandrogenemia may possibly disturb oocyte developmental potential .

With the recent understanding of cancer biology, physiopathology, and cancer genetics& genomics, new treatments and methodologies are constantly developed, including on the development and application of molecular targeted drugs for cancer therapy have been widespread concerned.Antibody-based drugs targeting over-expressed oncogenes or other functional proteins in cancer with its high specificity, less side-effects and significant clinical efficacy became rapidly the hot-spot of anti-tumor drug research.Currently, there are already more than 20 of anti-tumor antibody-based drugs approved for clinical application, and achieved remarkable results.This article summarizes the development and current situation of monoclonal antibody-based drug research for cancer therapy, as well as their anti-tumor mechanism of action.At the same time summarizes the new advances in cancer research in the field of monoclonal antibody drugs made,and its prospects as well as shortcomings were outlined.