0.05). CONCLUSION: The established quality standard can be used for the quality control of Pyrus ussuriensis.

ObjectiveTo explore the method of preparing the tube from chitosan and study the biocompatibility between blood and chitosan. Methods3％ chitosan-based hydrolgels were pro-duced from acetic acid and chitosan. The needles coated with chitosan were submerged in NaOHaqueous solutions. The tubes of chitosan were taken off from the needle. The portosystemic shuntswere created using the tube of chitosan. This model without heparin was observed whether the intes-tinal congestion developed or not. ResultsIt was shown that the chitosan-based tube has blood in 8rats went back to veinal system. ConclusionIt was easy to prepare the tube from chitosan. Thetubes have an excellent biocompatibility with blood.

Objective To investigate the safety and efficiency of using Succinylcholine as premedication for non-urgency intubation in prematurity.Methods During Jan.to Jul.2007,in Ottawa General Hospital and Children's Hospital of Eastern Ontario NICU,the prematurity who were received non-urgency intubation were divided into 2 groups as premedication group(group A)and non-premedication group(group B).Premedications in group A were applied Atropine,Fentanyl and Succinylcholine;premedications in group B were applied Atropine and Fentanyl.The same procedures were done according to the protocol in 2 groups.The basical information,one attempt successful intubation,total attempts,Goldberg scores and side effects were compared between both groups.Results Forty-four cases were involved in the study,25 cases in group A,19 cases in group B,there was no significant difference in patients' basical information between both groups.One attempt successful intubation was 56.0%(14 cases)in group A,higher than that in group B [21.1%(4 cases)],which had significant diffe-rence between 2 groups(P0.05).Conclusions Atropine,Fentanyl and Succinylcholine are safe as the premedication used in prematurity,which can improve one attempt successful intubation avoiding glottal injury of repeating attempts.It also can helps young physician to be familiar with the intubation techniques,premedication is recommended to teaching hospital.

Objective To study the genes modulated during plasma cell development and their regulating network. Methods The changes of gene expression during differentiation from mature B cells to plasma cells were studied using cDNA microarray and RT PCR. Results The gene expression profile during plasma cell differentiation was established. During mature B cell plasma cell differentiation, numerous genes were modulated, including genes involving immunity, cell growth, survival, and signal transduction. The expressions of several molecules in the B cell antigen receptor (BCR) signaling pathways, such as Ig?, Ig?, Lyn, Syk, BLNK, SWAP 70, and SHP 1 were down regulated. The decreased expression of some of these molecules might be associated with the down regulation of the transcription factor BSAP. Conclusion During plasma cell differentiation, molecules involving BCR signaling pathways are down regulated.

Erectile dysfunction (ED) results from the interaction of many pathological factors. Studies show that a high incidence of ED is associated with chronic diseases of various systems, and its pathogenesis is not fully understood. This article outlines the progress in recent studies on the impact of chronic diseases on erectile function.
Chronic Disease ; Erectile Dysfunction ; etiology ; Humans ; Male ; Penile Erection ; physiology

BACKGROUND: Studies showed cellular adhesion molecule and neurotrophic factor secreted from olfactory ensheathing cells (OECs) could protect the spinal neurons and promote the regeneration of spinal axon. OBJECTIVE: To compare the competence to repair spinal cord injury between olfactory mucosa OECs and olfactory bulb OECs. DESIGN, TIME AND SETTING: Randomized control animal experiment was performed in the central laboratory of Xidian Group Hospital between June 2007 and June 2008. MATERIALS: Twelve male SD rats were randomized selected and divided into experiment group (n=6, 23 months old) and control group (n=6, 3 months old). They were used for in vitro culture and purification of OECs; other 30 SD rats were randomized into three groups of 10 rats each: neonatal rat olfactory bulb OECs transplantation group, normal olfactory mucosa OECs transplantation group and blank control group.METHODS: Spinal cord injury models were produced in 30 rats, which were transplanted with the neonatal rat or SD rat OECs cultured in vitro. No transplant was given in the control group. MAIN OUTCOME MEASURES: At 4 and 8 weeks postoperation, the Basso, Beattie, Bresnahan (BBB) score for nerve function, the evoked potential of legs and the histopathological diversify of injured spinal cord. RESULTS: Seven rats died dudng the experiment process, and the death rate was similar between groups. At 4 and 8 weeks postoperation, there was no significant difference in the BBB scores between neonatal rat olfactory bulb OECs transplantation group and normal olfactory mucosa OECs transplantation group (P > 0.05), which were both significantly higher than blank control group (P < 0.001); the BBB scores in two transplantation groups were higher at 8 weeks than at 4 weeks (P < 0.01 ). At 4 weeks postoperaUon, no animal was shown to elicit motion evoked potential, but it was present in two transplantation groups at 8 weeks, with no significant difference between two groups (P > 0.05). The blank control group had still no motion evoked potential (P < 0.001 ). At 8 weeks postoperation, more cell infiltrations were found in the injured spinal cord of two transplantation groups, while few in the control group.CONCLUSION: Both OECs dissociated from olfactory bulb and olfactory mucosa have the same ability to repair the injured spinal cord, and their effect is similar.

BACKGROUNO:Some experiments have proved that some traditional Chinese medicines (TCM) have good biocompatibility when they touch and interact with human body tissues, body fluid or blood.Therefore,the physicochemical properties and functions of biomaterials can be improved by compounding it with some TCM.OBJECTIVE:To evaluate the biocompatibility of gelatin-bletilla porous material carrying TCM.DESIGN:Single sample experiment.SETTING:Department of Acupuncture & Moxibustion and Orthopaedics.Hubei College of Traditional Chinese Medicine. MATERIALS:This experiment was carried out in the Department of Orthopaedics,Tongji Medical College,Huazhong University of Science and Technology between February and April 2005.Twelve mice,male and female in half,weighing 18-24 g, and 6 Japanese big-eared rabbits,weighing 2.8 to 3.0 kg,were chosen for this experiment.They were raised at (18±1)℃ in separate cages.METHODS: Spongy gelatin-bletilla porous materials carrying extract of Coptidis and Radix Salviac Militiorrhizae was prepared by freeze-drying method.The acute systemic toxicity test:The 12 mice were divided into experimental group and control group. Mice in the two groups were intraperitoneally injected with leaching liquor and normal saline,respectively (the dose was 50 mL/Kg),then the general state and body mass of mice were recorded at the 4th,24th,48th and 72nd hours after injection, respectively.The porous materials were implanted in dorsal muscle of rabbits.Two rabbits were sacrificed at the 1st, 2nd and 6th weeks after operation seperately,and tissue reaction of biomaterial in vivo was assessed by gross observation and histological examination. MAIN OUTCOME MEASURES: Gross observation and histological examination of tissue reaction of biomaterials in vivo.RESULTS: Twelve mice and six big-eared rabbits were involved in final analysis.The acute systemic toxicity test:Mice of two groups had good status,normal activity and appetite,stable respiration:and no abdominal irritation,exhaustion, cyanosis or death was found.Body mass of mice of two groups presented tendency of increase at the 24th,48th and 72nd hours after injection,and there were no significant differences between two groups (P＞0.05).The implantation test:①Gross observation:The samples were tightly bounded with muscular fibrous tissues at postoperative 1 week.They were connected with muscular fibrous tissues and could not be easily separated at postoperative 4 weeks.Gelatin-bletilla porous materials carrying TCM could hardly been seen at postoperative 8 weeks.②Histological observation:There were a lot of infiammatory cells and mesenchymal stem cells around the samples at 1 week after the implantation of gelatin-bletilla porous materials carrying TCM; Some materials were degraded and infiammatory cell infiltration was reduced around the implanted materials at 4 weeks; The inflammatory cells were further reduced and the materials were absorbed and replaced by muscular tissues at 12 weeks.CONCLUSION:Gelatin-bletilla porous materials carrying TCM is safe and reliable for application in wound healing,skin and muscle tendon tissue engineering.

Objective To observe the distribution of circum-patella nerve fibers in the soft tissue to provide experimental evidence,which is significant in denervation for Total Knee Arthroplasty (TKA).Methods Patella specimens were collected from 4 cadavers (2 cases of diabetic foot,lcase of lower extremity arterial occlusive,and 1case of car accident),all 4 of which were resected soft tissue with a dimension of 0.5cm × 0.5cm and full depth thickness around patella more than 0.5cm for histology and morphology observation.The nerve fibers histology and morphology were observed in all resected specimens with HE staining and silver-gilt glycine staining in the same field of microscopic vision.Results Anatomy found that the vascular network form skins directly involved in the patella nourish hole area and in the 10,2,4,7 clock point have found that blood vessels into the patella.There have a large number of nerve fibers near to the patella under the microscope,but there were no significant difference in the nerve fibers region distribution of all specimens.There were some into patella nerve fiber paths in side of patella soft tissue,which lied in 7,11 and 13 clock point,but outside no this phenomenon.The distribution of circum-patellar nerve fibers were described as distribution of regional concentration,which lied in much more 5,6,7 clock points and 10,11,12,1,2,clock points,in which the quadriceps tendon and patellar tendon have more than the others.In the 13 clock point,the fascia and periosteum of nourish hole area were also found in a large number of nerve fibers,and there were laminar distribution in different soft tissue layers,which were collected much more in synovial layer,fat pad,tendon near to patella.Conclusion There are much more nerve fibers near to the patella and some into patella nerve fiber paths in the medial side and nourish hole area.Nerve fibers distribution of circum-patella can be described as laminar distribution and regional concentration ,which is more in the centre,bottom more than top,outside more than inside,the bipolar more than the others.The patella denervation operation by reducing the number of peripheral nociceptors to achieve desensitization is feasible in TKA.

Objective To investigate the etiology, diagnosis and treatment of pneumocystis cari-nii pneumonia (PCP) at early stage after orthotopic liver transplantation (OLT). Method The clinical data of 276 patients undergoing OLT were retrospectively analyzed and those of 6 cases complicated with PCP were summarized from January 2005 to December 2005. Results The morbidity of PCP in our group was 2.17% and occurred in early stage after OLT. The average time on set was (11.17?2.50) days and uneasily controlled hypoxemia was the main manifestation. The diagnosis of PCP was established by clinical symptoms, chest X-ray, chest CT, pneumocystis carinii PCR detection and visualization of PC encysts from sputum samples under a microscope. SMZco was the initial choice of treatment combined with immunosuppressive regimen regulation. Five cases were cured and 1 case died from septic shock. Conclusion PCP can occur among OLT patients in the early stage, and aggressive early diagnosis and treatment were critical to improve the prognosis.

Objective: To establish an HPLC fingerprint spectrum of Mongolian medicine Rubus sachalinensis. Methods: An HPLC was performed on the column of phenomsil ODS (250 mm × 4. 6 mm,5 μm) with the mobile phase of 0. 3% phosphoric acid-MeOH with gradient elution at a flow rate of 1. 0 ml·min-1 ,the detection wavelength was 290 nm, the column temperature was 25 ℃and the injection volume was 20 μl. Totally 7 batches of Rubus sachalinensis from different habitats were analyzed. Results:The RSD of relative retention time of the common peaks of Rubus sachalinensis was less than 3%, and that of relative peak areas was below 3%as well, which were in accordance with the requirements of fingerprint. Conclusion:The established HPLC fingerprint has promising accuracy, repeatability and stability, which can be used as one basis for the quality control of Rubus sachalinensis.