Objective: To investigate the expression ofVEGF-Aand the correlation with the sentinel lymph nodes lymphangiogenesis and its metastasis in non-small cell lung cance(rNSCLC).Methods: The expression of VEGF-A was detected in 65 patients with NSCLC by SP immunohistochemistay. The expression of LYVE-1 was detected by immunofluorescence. The new lymphatic vessel density was counted by LYVE-1 and analyzed with clinicopathologic parameters. Results: The positive rate of VEGF-A was 72.3%(47/65),which was positively correlated with sentinel lympha nodes metastasis(r=0.453,P

0.05).Conclusion:QDs show no effect on cytobiological behavior of living MG-63 cells.

Adult ; Anaphylaxis ; etiology ; Female ; Humans ; Male ; Semen ; immunology

BACKGROUND:After cerebral ischemia/reperfusion injury,as a executioner caspase, procaspase-3 and caspase-3-like activity increased significantly. We observe both the dephosphorylated and phosphorylated procaspase-3, and try to find out their variations during the processes of cerebral ischemia/reperfusion injury.OBJECTIVE:Observe the expression of procaspase-3 in hippocampus following transient forebrain ischemia.DESIGN: A randomized and controlled experiment.DEPARTMENT:Department of Biochemistry and Molecular Biology,Capital University of Medical Sciences.SETTING:Department of Hyperbaric Oxygen of Beijing Chaoyang Hospital Affiliated to Capital University of Medical Sciences.METHODS:Transient forebrain ischemia was induced by bilateral common carotid occlusion (BCCAO) for 20 minutes. Hippocampus was obtained at reperfusion time points of 6 hours, 12 hours,24 hours and 48 hours respec-tively after 20 minutes of BCCAO. Sham-operated group did not occlude bi-lateral common carotid, and hippocampus was obtained at reperfusion time point of 24 hours. Western Blotting was used to detect the level of procaspase-3.MAIN OUTCOME MEASURES:The comparison of total procaspase-3,dephosphorylated procaspase-3 and phosphorylated procaspase-3 level in hippocampus between each group.3 level: Total procaspase-3 level increased in hippocampus at reperfusion time points of 12 hours and 24 hours post-BCCAO (9 133.1 ±2 216.3,dephosphorylated procaspase-3 level increased in hippocampus at reperfusion time point of 24 hours post-BCCAO (7812.0±1625.1, 3825.8±155.6, P was not significant (P ＞ 0.05) as compared with the expression levels in sham-operated mice.CONCLUSION: Procaspase-3 is upregulated after ischemia/reperfusion.The increment of dephosphorylated form of procaspase-3 was higher than that of phosphorylated form of procaspase-3 upon cerebral ischemia/reperfusion injury, which indicates that cerebral ischemia/reperfusion injury possibly induced the dephosphorylation of procaspase-3 and promoted its transforming into activated form.

OBJECTIVE: Superoxide dismutase is one of the families of enzymes which is ubiquitous, and efficiently catalyzes the dismutation of superoxide anions.There are three superoxide dismutases in human brain that are of benefit to ischemic damage. In recent years, transgenic and knockout mutant mice has been widely used in the researches of superoxide dismutases for its protective effect in cerebral ischemia.DATA SOURCES: Using the terms "Chan PH and superoxide dismutase and cerebral ischemia", the relative articles published in the English from January 1991 to December 2003, were identified by searching the MEDLINE database with computer.STUDY SELECTION: The trials with transgenic and knockout mutant mice were identified preliminarily, then all remainders were searched in full content and were divided into intervention group and controlled group, the random cases were accepted, non-random excluded.DATA EXTRACTION: A total of 38 fully studies related to cerebral ischemia and superoxide dismutase were collected, among them, 13 random cases were accepted, and 25 excluded which included 15 repeated studies and 10 irrelevant studies.DATA SYNTHESIS: Reactive oxygen species involved in redox signaling pathways in ischemic cerebral, it can also cause oxidative damage of some macromolecules cell such as lipids, proteins and neucleic acid. In the ischemic brain tissue, overproduced oxygen radicals and consumption of superoxide dismutases lead to tissue damage. Copper-zinc superoxide dismutase, manganese superoxide dismutase and extracellular superoxide dismutase have the same function which can decompose superoxide anion radicals to form H2O2 that are scavenged further, thereby ameliorate edema formation,infarction and cell death, improve the function of ischemic cerebral tissue further.CONCLUSION: Increased oxygen free radicals is an important mechanism in ischemic cerebral damage, all three SOD can ameliorate cerebral ischemia. The protect effect of three superoxide dismutase is related to DNA repairing enzyme, transcription factor, apoptosis proteins and signal transduction pathway.

After a description of the concept and meaning of big data, the application of big data in biomedical field was investigated, the changes in medical library functions were analyzed, the challenges to medical libraries were pointed in terms of their data, hardware infrastructures, software technologies, talents and service modes, and how to respond to the big data in medical libraries was proposed from the aspects of strengthening the external collabo-ration, co-developing cloud computing center, intensifying the construction of related organizations and talents, and changing the service modes.

Objective To investigate the incidence of abnormal thyroid function in pregnant women and explore the significance of thyroid function screening in early pregnancy. Methods We randomly selected 630 cases of pregnant women who received routine inspection in the obstetric clinic in our hospital, and gave them five items of thyroid function screening besides routine check at the same time. Then we statistically analyzed the test results, and followed up 61 cases of pregnant women with hypothyroidism or subclinical thyroid function (hypothyroidism) to learn the treatment and adverse pregnancy outcomes,so as tto explore the correlation between hypothyroidism, subclinical hypothyroidism and adverse pregnancy outcomes. Results In 630 cases of pregnant women,there were 8 cases of hyperthyroidism (1.3%),61 cases of hypothyroidism or subclinical hypothyroidism (9.7%),including 5 cases of hypothyroidism (0.8%), 56 cases of subclinical hypothyroidism (8.9%) .There was statistically significant difference in TSH and FT4 between hypothyroidism group and normal control group ( <0.01) .There was statistically significant difference in TSH,FT4 and TT4 between subclinical hypothyroidism group and normal control group ( <0.05) . In 61 cases of hypothyroidism and subclinical hypothyroidism, 39 patients received levothyroxine therapy,22 received no treatment. The incidence of adverse pregnancy outcomes in the treatment group was significantly lower than the untreated group ( <0.05) . Conclusion Thyroid function testing in 6-20 weeks in early pregnancy has important significance in timely founding gestational thyroid dysfunction, so it should be as routine examination project.

Objective To analyze the effect of Kangfuxin Liquidon on gingiva groove liquid interleukin 1β(IL-1β), prostaglandin E2 (PGE2), soluble adhesion molecule-1 (sICAM)in fixed orthodontic patients with gingivitis. Methods 96 cases of patients with fixed orthodontic gingivitis consult the draw method were divided into control group and experimental group, 48 cases in each group. The control group were treatedby gums clean, experimental group based on the control group were treattedby Kangfuxin Liquidon. The IL-1β, PGE2, sICAM-1 levels, periodontal status, the grade of swelling and pain, the clinical curative effect were compared between two groups. Results After treatment, the IL-1β, PGE2, sICAM levels of experimental group were lower than the control group (10.54±1.41) ng/L vs.(11.85±1.71)ng/L, (284.62±35.21) ng/L vs.(314.65±39.48)ng/L, (150.49±18.11) μg/L vs.(162.83±20.26) μg/L,the differences were statistically significant (P<0.05).The periodontal status, swelling and pain grading ofexperimental group were better than control group (P<0.05). Theeffective rate of experimental group was higher than the control group (95.83%vs.79.17%) (P<0.05). Conclusion Kangfuxin Liquidon can reduce fixed orthodontic patients with gingivitis gingiva groove IL-1β, PGE2, sICAM-1 levels, improve the periodontal status, relieve swelling and pain, improve the clinical curative effect.

Recurrence after surgery for common bile duct stones has not been solved properly,and recurrence of calculi often requires reoperation,which brings patients great pain and economic burden.Various risk factors may mediate the recurrence of common bile duct stones,including biliary tract infection,bile duct stenosis,cholangiectasis,cholestasis,Oddi sphincter dysfunction,abnormal bile composition,and surgical procedure.These risk factors are analyzed and related preventive measures are summarized.Current research findings show that prevention and treatment of risk factors for recurrence of calculi can reduce the recurrence rate of calculi and play an important role in preventing the recurrence of common bile duct stones.

OBJECTIVE:To enhance the competitive edge of Chinese pharmaceutical enterprises.METHODS:Patents problems confronted by Chinese pharmaceutical enterprises were analyzed in terms of the research and development and patent protection of the original new drugs,the administrative protection and patent protection of drugs,patent documentation in?formation,patent strategy and patent talents,etc.RESULTS&CONCLUSION:Chinese pharmaceutical enterprises should highlight the patent problems and apply patent strategy to achieve competitive advantage.