The present paper reported that a murine IgM monoclonal antibody named Np30, which was proved to be an internal image of a gut associated antigen molecule of S. japonicum in function , was labelled with HRP and used to detect the antibody response of individuals acutely and chronically infected with S. japonicum by means of Dot-ELISA, a direct detection assay. The results showed that the test employed in this study was statistically comparable in effect to conventional EL1SA for the diagnosis of schistosomiasis japonica, but much simpler in operation and easier for the field use.

Object To study the phsiochemical properties of storax ? cyclodextrin (? CD) inclusion complex Methods The inclusion complex was identified by the methods of TLC, X ray powder diffractometry and IR The solubility and dissolution rate of cinnamic acid in inclusion complex were investigated by HPLC Results The TLC showed that the main composition of storax had no change before and after being included by ? CD The spectra of X ray powder diffractometry and IR of the inclusion complex were remarkably different from those of storax and storax ? CD mixture It was shown a great improvement of the solubility and dissolution rate of cinnamic acid in the inclusion complex in 0 1 mol/L HCl, pH 6 6 and pH 7 5 phosphate buffer solution Conclusion The storax ? CD inclusion complex exhibits some new physical characteristics and its physiochemical properties are greatly changed comparing with those of storax

Objective To study the effect of rhu-IFN-? on immune state and vertical transmission in pregnant mice infected with Toxoplasma gondii. Methods Sixty pregnant BALB/c mice were randomly divided into three groups: a control group,infected group and treatment group. In the infected group and treatment group,each mouse was injected with T.gondii tachyzoites peritoneally on the day 8 of gestation. In the treatment group,each mouse was treated with 1 000 U rhu-IFN-? on the day 7,8,9 of gestation. Blood was collected from the tail veins of all the mice on the day 10,12 of gestation. The levels of blood CD4+ and CD8+ T cells were detected by flow cytometry. Meanwhile,on the day 12 of gestation,all mice were anatomized to observe live embryo rate and the infection status in fetal brain tissue. Results Compared with the control group,the levels of CD4+ T cells in the infected group and treatment group were low,and the levels of CD8+ T cells high on the day 10,12 of gestation,so the ratio of CD4+/CD8+ was inversed. However,compared with the infected group,the levels of CD4+ T cells in the treatment group were high and the levels of CD8+ T cells low on the day 10,12 of gestation,so the ratios of CD4+/CD8+ were high on the day 10,12 of gestation. Meanwhile,the live embryo rate was high and the infection rate of intrauterine embryonic low. Conclusion A proper dose of rhu-IFN-? could improve the function of immunity system and reduce the vertical transmission probability in pregnant mice infected with T.gondii.

In this study the ability of the monoclonal anti-idiotypic antibody NP30 was tested as a substitute of diagnostic antigen in detecting antibody of Schistosoma japonicum from human sera by use of ELISA. The results showed that the seropositive rate was 98% with NP30 in the group of acute infection, which was comparable to 94% with gut associated antigens (GAA)and 98% with the soluble egg antigens (SEA); 87% with NP30 in the group of chronic infection which was comparable to 86% with GAA but lower than that of 98% with SEA. The false positive rate was about 3% for all three diagnostic antigens. The results also showed that the geometric mean titer (GMT) of antibody to NP30 was higher than that to GAA but lower than that to SEA in the acute infection group and the GMT of antibody to NP30 was lower than both those to GAA and to SEA in the chronic infection group,suggesting that the antibody to NP30 appeared earlier and decayed more quickly during the process of infection. The authors suggested that NP30 could be used for the diagnosis of schistosomiasis japonica.

Object To investigate the release of pH dependent gradient releasing heart protecting musk pellets (GRHPMP) in vitro. Methods The pH dependent GRHPMP was prepared by coating with hydroxy propylmethy cellulose, Eudragit ○R L 30D 55 and Eudragit ○R L100/S100, repectively. The release of borneol and total ginsenoside from GRHPMP were determined according to method described in Chinese Pharmacopoeia (2000 ed) at simulated gastrointestinal pH conditions. Results The f 2 value of release data of borneol and total ginsenoside was 79 6. Conclusion The result suggested that, in vitro, the liposoluble borneol and watersoluble total ginsenoside could release simultaneously at a sustained rate.

Object To prepare Heart protecting Musk Pellet (HPMP). Methods The HPMP was prepared by using a centrifugal granulator. The effect of formulation and technology on the yield of pellets was investigated. The optimal conditions of technology were selected by the uniform design. Results The yields of core pellets were affected significantly by the rotating rate of plate, amount of adhesive agent, the added speed of adhesive agent and the conditions of nebulization. The spraying rate of binder solution and feeding rate of powder were found to have significant influences on pelletizing. The process parameters established by the uniform design were as follows: the ratio of fine intermediate product and MCC was 1∶1, the adhesive agent was 3% HPMC solution. The rotating rate of plate was 200 r/min, the blower rate was 10?20 L/min, the rate of air flow was 15 L/min, the spray air pressure was 0 5 MPa, the rotating rate of spray solution pump was 14 r/min and the rotating rate of powder feed machine was 18 r/min. Conclusion Under the optimal conditions, the pellets prepared by using centrifugal granulator have perfect shape and surface characteristics and the yield of 1 000 700 ?m pellets could reach to 90 4%.

BACKGROUND:In early experiments, we prepared hydroxyapatite-coated mechanical heart valve embedded with col agen using impulse laser sediment method.
OBJECTIVE:To further analyze the histocompatibility and toxicity of hydroxyapatite-coated mechanical heart valve embedded with col agen.
METHODS:After passage, canine vascular endothelial cellsuspension was inoculated onto the hydroxyapatite-coated mechanical heart valve embedded with col agen. One group was inoculated in 5%CO2, 37 ℃ incubator for 3 weeks static culture, and the other group was inoculated in 5%CO 2 , 37 ℃ incubator for 3 weeks spinner culture. Scanning electron microscope was used to observe cellattachment on the material. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to the proliferative capacity of vascular endothelial cells cultured with the hydroxyapatite-coated mechanical heart valve embedded with col agen.
RESULTS AND CONCLUSION:During the spinner culture, adherent cells were found on the surface of mechanical heart valve, and the cells distributed evenly and confluent at 21 days to cover the surface of the material. The number of adherent cells in the spinner culture was higher than that in the static culture. The cells during the static culture were aggregated and distributed irregularly. The mechanical heart valve exhibited no effects on the proliferation of canine vascular endothelial cells which grew wel . These findings indicate that the hydroxyapatite-coated mechanical heart valve embedded with col agen exert no effect on proliferation of vascular endothelial cells, has no toxicity and has good biocompatibility.

Object To investigate the pharmacokinetic parameters of Musk Protecting Heart Pellets with pH-dependent gradient-release (MPHP-pH) and Musk Protecting Heart Pills (MPHP). Methods The cardiac muscle nutritional blood flow in rat was measured as effective index. Results It was one-compartment model when the rat was ig MPHP. The minimal effecting dose was 0.54 mg/kg, the present half-life, the elimination half-life, medicinal effect and the peak time of effective action were 0.53, 1.21, 3.48 and 1.13 h, respectively. The absorption half-life, elimination half-life and peak time of effective dose were 0.23, 1.47 and 0.88 h, respectively. Statistical moment analysis showed that the mean residence time mean residence time (MRT) of effective action were 5.05 h for MPHP-pH and 2.33 h for MPHP, the MRT of effective dose were 7.70 h for MPHP-pH and 3.21 h for MPHP. The relative bioavailability of effective dose for MPHP-pH was 104.03%. Conclusion MPHP has the characteristics of fast absorption, fast eliminationand short effective action time. Whereas MPHP-pH has the characteristics of fast absorption, protonged and relaxed effective action compared with MPHP.

Objective To observe the effect of self-developed simple arteriovenous haemostat on compression hemostasis of the puncture point after withdrawing needlesfor venipuncture. Methods 160 inpatients with transfusion in the inpatient area of Neurology Department who are admitted from April 2015 to June 2015 were selected as the subject of study, and they were randomly divided into the control group and the observation group, with 80 cases for each group. The patients in the control group weretreated with conventional compression hemostasis, and they were continuously compressed for 5 minutes, while the observation group was treated with the simple arteriovenous haemostat (patent number ZL 201520184173.3) to make compression hemostasis. The nurses operated the haemostat for about 30 to 50 seconds, without waiting beside the patients. The haemostat was removed after 5 minutes. The incidence of subcutaneous congestion and puncture point bleeding, as well as the nurses' time on the local compression hemostasis of the puncture point and the comfort level and satisfaction of patients were compared between the two groups. Results The control group was 5 cases (6.25％) of the puncture point bleeding, 9 cases (11.25％) of subcutaneous congestion, 48 cases (60.00％) of comfortable feeling, 22 cases (27.50％) of common feeling, 10 cases (12.50％) of discomfort, 47 cases(58.75％) of satisfaction, 24 cases (30.00％) of common satisfaction and 9 cases (11.25％) of dissatisfaction. The observation group was 0 case of the puncture point bleeding, 2 cases(2.50％)of subcutaneous congestion, 77 cases(96.25％)of comfortable feeling, 2 cases(2.50％)of common feeling, 1 case(1.25％)of discomfort, 70 cases(87.50％) of satisfaction, 8 cases (10.00％)of common satisfaction and 2 cases(2.50％)of dissatisfaction. The observation group was better than the control group in compression hemostasis time, subcutaneous congestion, punctures point bleeding and satisfaction and comfort level, and the difference was statistically significant (P < 0.05). Conclusions The strength, position and time of compression hemostasis by simple arteriovenous haemostat are controllable, visual and constant, which can achieve the effect of standard compression by nurses, and has the value to be popularized in clinical practice.