Objective To study the relationship between P53 and carcinogenesis of epithelial ovarian cancer. Methods Immunohistochemistry was used to evaluate the expression level of P53 protein in 74 cases of epithelial ovarian cancer. Results The expression level of P53 protein was higher in G1 and G2 groups (90.62%) than in G3 group (66.67%, P 0.05). Conclusion P53 may play a role in the pathogenesis of epithelial ovarian cancer.