AIM: To explore the effect of collagen and C-erbB-2 protein on the adhesion and the proliferation in hepatocellular carcinoma cells.METHODS: Hepatocellular carcinoma cell line(HepG-2) identified to positive for C-erbB-2 gene was used to study the adhesion and the growth feature by the action of rat tail collagen and C-erbB-2 antibody.RESULTS: The action of rat tail collagen to potentiated the adhesion in HepG-2 cells was significantly but no proliferation effect was observed. C-erbB-2 antibody inhibited the adhesion and proliferation of HepG-2 cells and also abolished the potentiated effect of rat tail collagen on the adhesion in HepG-2 cells.CONCLUSIONS: The signaling transduction mediated by C-erbB-2 protein was correlated to the adhesion and the proliferation of HepG-2. The blockage of C-erbB-2 gene signal transduction may be a strategic target to the treatment of liver cancer in the future.

OBJECTIVE: To compare the results between auditory steady-state response (ASSR) and 40 Hz auditory event related potential (AERP), and explore the accuracy of hearing thresholds by using ASSR and AERP and the clinic forensic value. METHODS: Thirty seven ears were tested with pure-tone audiometer, 40Hz AERP and ASSR, respectively. All the volunteers in our study were awake during 40 Hz AERP test and ASSR test. RESULTS: Thresholds acquired with ASSR and 40Hz AERP test had a close correlativity and showed higher than those acquired with PTA test. There was no significant difference between the accuracy of ASSR and 40Hz AERP in estimating pure-tone thresholds. CONCLUSION After determining the correct value, ASSR can be used directly to evaluate hearing loss objectively.
Acoustic Stimulation ; Adult ; Audiometry, Evoked Response ; Audiometry, Pure-Tone/methods* ; Auditory Threshold ; Evaluation Studies as Topic ; Evoked Potentials, Auditory, Brain Stem/physiology* ; Female ; Hearing Loss, Sensorineural/physiopathology* ; Humans ; Male ; Middle Aged ; Predictive Value of Tests ; Sleep/physiology* ; Wakefulness ; Young Adult