Objective To explore the relationship between the inhibiting effect of arsenic trioxide(As 2O 3) on tumor and nm23 gene expression. Methods The human gastric carcinoma cell line was treated with As 2O 3. Then immunohistochemistry was used to detect nm23 protein expression. Results As 2O 3 up-regulated the expression of nm23 in gastric carcinoma cells. Conclusion The inhibiting effect of As 2O 3 on gastric carcinoma cells may be related with upregulation of nm23 expression, which decreases the gastric cancer cells metastatic ability.

Objective　To observe the effects of repeated subconvulsive electrical stimulations to hippocampus on rat's emotional behavior and spatial learning and memory. Mehtods　The hippocampus of Wistar rats were repeatedly stimulated by constant pulsating current with intratrain frequencies of 16 Hz, pulsating duration of 1 ms, train duration of 10 s and interstimulus interval of 7 min for 5 days. Results　The experimental rats displayed the long-term significant abnormalities of emotional behaviors, such as substantial changes of behavioral habits, hyperarousal, hypersensitive frightened reactions, anxiety-like behaviors, and transitory spatial learning and memory disorder. Conclusion　The stable and practical animal model satisfied most characteristics of posttraumatic stress disorder (PTSD).

There are also many unanswered questions on the duodenogastroesophageal reflux(DGER),though it has been studied for more than 100 years.Based on the references and our studies,we reviewed the bile' injury on esophageal mucosal epithelial cells,the diagnosis and treatment of bile reflux into esophagus.The relationships of the biles with Barrett's esophagus and esophageal adenocarcinoma were elucidated especially.

ObjectiveTo investigate the encountered life events in patients with tuberculosis within 1 year before the attack.MethodsThe encountered life events within 1 year before the attack were investigated in 85 patients with tuberculosis, the life event frequency (LEF) and life event intensity (LEI) were calculated respectively, and the life events were compared with those within 1 year in 55 healthy subjects.Results71 cases (83.5%) of the 85 patients with tuberculosis had encountered different life events within 1 year before the attack, which was significantly different from that in the control group (9 cases, 16.4%) (χ2=12.351,P<0.01 ). The LEF, negative LEF, average LEI, average negative LEI in the tuberculosis group before the attack were obviously higher than those in the control group (P<0.05～0.01), but there were no significant differences in the positive LEF and average positive LEI between the two groups (P>0.05).ConclusionThe encountered life events are closely correlated with primary tuberculosis, so more mental cares should be paid to the patients.

Alternative splicing of pre-messenger RNA (pre-mRNA) is a crucial mechanism for the diversity of the human transcriptome and proteome. Alternative splicing is a complex gene regulation process. Whole-transcriptome analysis shows that 95% of human exonic genes are alternatively spliced, involving various cis-acting elements and trans-acting factors. Any changes in any component or step may cause erroneous splicing events and lead to the occurrence of various related diseases. In addition to gene replacement therapy that directly changes the splicing results, RNA splicing modification is expected to become a new therapeutic strategy to alleviate or treat diseases by targeting and correcting abnormal pre-mRNA splicing. Splicing modification tools currently developed including RNA trans-splicing, antisense oligonucleotides, small interfering RNA, and small molecule drugs can correct abnormal splicing through different ways. This article reviews the resent progress of epigenetic regulation of pre-mRNA alternative splicing in recent years, and discusses the occurrence and regulation of alternative splicing, the types of diseases caused by related splicing defects, and the current-used tools for targeting and altering splicing. The importance of splicing modification strategies in the future treatment of human diseases is envisioned.

Objective To investigate the comparison of blood uric acid and bilirubin levels on patients with acute cerebral infarction and the clinical curative effect of Donhong injection.MethodsA total of 68 patients with acute cerebral infarction from our hospital neurology, according to different therapeutic methods were divided into control group and experimental group with 34 cases in each group, two groups were treated by conventional treatment, patients in the control group were treated by blood flux injection 400 mg+5% glucose injection 250 mL intravenous drip, once a day;patients in the experimental group were treated by Dan red injection 40 mL+5% glucose injection 250 mL intravenous drip, once a day,2 groups of patients were treated for 2 cycles,a cycle with 7 d.Compared the clinical efficacy, blood uric acid, bilirubin, blood homocysteine, hyperensitive C-reactive protein, serum inhibition C and adverse reactions in the two groups after treatment.ResultsCompared with the control group, the experimental group clinical total effective rate was higher after treatment (P< 0.05).Compared with the control group, the experimental group blood uric acid, blood homocysteine, hypersensitive C-reactive protein, serum inhibition C levels were lower (P< 0.05), bilirubin level was higher(P< 0.05).Adverse reactions compared between the two groups, there was no statistically significant difference.ConclusionDonhong injection in the treatment of acute cerebral infarction clinical curative effect is distinct, dacrease blood uric acid level and increase bilirubin level, improve endothelial cell damage, protect the brain tissue.

Objective To study mechanism and clinical meaning of endothelin(ET) and neuron specific enolase (NSE) in patients with acute stroke.Methods The contents of ET and NSE in blood and cerebrospinal fluid were measured by RIA in patients with acute stroke, and correlation analysis of the contents of ET and NSE with different lesion area,severe degree and different period of the disease was performed.Results The contents of ET and NSE in acute cerebral hemorrhage and cerebral infarction groups were significantly higher than those in control group ( P

Acupuncture Therapy ; Adult ; Bulbo-Spinal Atrophy, X-Linked ; therapy ; Humans ; Male

Objective That decline of estradiol in serum of postmenopausal women can result in dry eye has been accepted at present. However, the change of estradiol in tear of postmenopausal women has been not reported ever. This study was to discuss the relationship between dry eye syndrome and estradiol in tears and serum of postmenopausal women. Methods Total 62 eyes of 62 women were collected in this trial. The aim of this trial was informed orally and the informed consent was obtained from all the subjects. The 32 right eyes were from menopausal women for more one year and 30 right eyes were from premenopause women. The dry eye was diagnosed based on the abnormalities of tear break-up time (BUT), Schirmer I test, corneal fluorescence and subjective symptom of patients. The periphery blood specimen was collected from all the subjects, and tear specimen was obtained and quantified using filter paper. Estradiol contents in tears and serum were determined by chemiluminescence method. The bulbar conjunctiva was obtained by impression cytology, and the microstructure of conjunctiva was observed under the scanning electron microscopy. Results In comparison to the premenopause group, the content of estradiol in tears and serum in menopause group was significantly reduced(73. 50 ±24.47 vs 17. 43 ± 10. 92) (U = -6. 396, P =0. 001). In the premenopause group, the estradiol content between tear and serum showed the positive correlation (r = 0. 838, P = 0. 001) . However, no statistical correlation was found in estradiol content between tears and serum in menopause group(r =0. 127, P = 0. 487). The maicrostructure changes were seen in the dry eyes of postmenopausal women, and decrease of goblet cell and loosen of intercellular junction were found under the electron microscope. Conclusion The content of estradiol in tears in menopause group is significantly lower than that in the premenopause group. Estradiol content in tears and serum of the postmenopausal women showes insignificant correlation. This result indicates that the topical substitute therapy with estradiol is possible in dry eye.

Abstract Tibet Plateau is one of the most prevalent areas of brick-tea type fluorosis in China. Effective management of brick-tea type fluorosis is a key point and a difficulty in endemic disease prevention and treatment in Tibet. Brick-tea type fluorosis is a human health concern that occurs under specific natural environments and social humanistic conditions in Tibet. Recently, the prevalence of brick-tea type fluorosis has been effectively reduced in the Tibet Plateau; however, there are still challenges for the overall brick-tea type fluorosis control, and the endemic status remains more severe than other regions endemic for brick-tea type fluorosis in China. Previous studies have shown that intake of high-fluoride brick-tea, dietary habits of drinking tea and specific natural environments are strongly associated with brick-tea type fluorosis in the Tibet Plateau. This review summarizes the advances in the epidemiological characteristics and influencing factors of brick-tea type fluorosis, so as to provide insights into the development of the long-term control strategy for brick-tea type fluorosis in the Tibet Plateau.