Objective To investigate the effect of hypertonic saline on TNF-а, IL-1β, S-100β level following focal cerebral ischemia-reperfusion injury in rats and explore the mechanisms involved. Methods Ninety rats were randomized equally into 4 groups,namely the pseudo-operated group (A group),untreated IR injury group (B group),C group (4.2% hypertonic saline) and D group (7.5% hypertonic saline). Cerebral ischemia was induced by middle cerebral artery occlusion for 2 h followed by administration of the corresponding treatments in group C and D. After 22 h of reperfusion , TNF-а, IL-1β, S-100βexpression in the ischemic brain tissue were measured by enzyme-linked immunosorbent assay (ELISA). Results After hypcrtonic saline treatment, the two saline-treated groups showed significant reduction in TNF-а、IL-1β、S-100β levels , but were still higher than pseudo-operated group(A group). Compared with B group, significant difference can be seen among C and D group. Conclusion Hypcrtonic saline can reduce cerebral TNF-а、IL-1β、S-100βlevel of isehemia-reperfusion injury.

To evaluate the effects of esmolol hydroehloride on hemodynamies during tracheal intubation. Method: One hundred patients undergoing elective cerebral, thoracic or abdominal operations, were randomly divided into three groups: Group A, Group B and Group C, which were injected I. V. esmolol 1.0mg/kg, 2.0mg/kg and normal saline 10ml respectively before induction of anesthesia. SP, DP, HR and RPP were recorded immediately before administration, before intubation and 1,2,3,5 and 10 min after intubation. Result: HR in Group A and Group B was signifcantly slower than in Group C, especially 3 min and 10 min after intubation(P

Objective: To investigate the effect of propofol on ischemia reperfusion injury in isolated rat heart with the modified Langredorff model. Method: Thirty rats were divided equally into five groups at random. Rat hearts were perfused with Krebs-Henseleik(K-H)buffer for 70 min at a constant pressure of 7.84kPa and constant temperature of 37℃ in control group (group C)and in the other four groups,a three-phase protocal was performed: (1)20-minute preperfusion, (2)30-minute global normothermic(37℃)ischemia, (3)30-minute reperfusion. Treatment with 50?mol/L propofol(group P1),25?mol/L propofol(group P2), 90?g/ml intralipid (group IN )dissolved in K H buffer started 10 minutes before ischemia and throughout the experiment. Only K-H buffer was perfused in the ischemia-reperfusion group(group I-R). The heart rate(HR),left ventricular pressure (IVP)and it's first derivative(?dp/dtmax) and coronory flow (CF)were recorded at the tenth and twentieth minute of preperfusion, and the 30th minute of reperfusion. Creatin kiuase (CK)activity was measured in the coronory effluent at the 30th minute of reperfusion. Result: After 30-minute reperfusion, recovery of hears treated with propofol were better than that of group I-R and group IN,indicated by better contractivity, higher coronory flow and lower CK level (P

Two glycan peptides, GLSP2 and GLSP3 were obtained from the hot-water extract of thefruit-body of Ganoderma lucidum. Gel chromatography and HPLC showed that they are bothhomogeueous polysaccharides,with molecular weights of 12800 aud 14100 respectively. Throughthe procedures of total acidic hydrolysis, periodate oxidation, Smith degradation, and spectraldetermination, GLSP2 proved to be a glycan peptide with ? (1→3 ) (1→6 ) and (1→4 )linkage, 26.6% of which is peptide; while GLSP3 proved to be a glycan peptide with ?- (1→6 ) and (1→4 ) linkage, aud the sugar residue ratio in these two bonds is 1∶1. The chain ofGLSP3 has branches, and peptide takes 12.3% 0f it. Basic ?-elimination reaction iudicatedthat the sugar residues in the two glycan peptides are linked with O-bond to serine and threonine on the peptide chain.

Objective To evaluate the imaging features of acute necrotizing encephalopathy caused by children A H1N1 influenza.Methods CT and MRI brain imaging data of three children acute necrotizing encephalopathy caused by A H1N1 influenza virus and proved by clinical and laboratory investigation were analyzed.All the three children got CT and MRI scan because of coma while in hospital.Results All cases represented multifocal damage,and some were symmetrical.Bilateral thalamencephalons were involved in the all cases.Bilateral grey and white matters of frontal lobe,temporal lobe,parietal lobe and occipital lobe,and hippocampi,putamina,external capsule,brain stem,bilateral cerebellum,could be seen multifocal damage,and some were symmetrical.All the cases showed brain swelling in varying degrees.These region presented low density in CT,hypointensity on T1 weighted series and hyperintensity on T2 weighted series and FLAIR series in MRI.Conclusion The imaging features of acute necrotizing encephalopathy caused by children A H1N1 influenza represented multifocal and symmetrical damage and brain swelling.It may be characteristic that bilateral thalamencephaloas were involved.The patient was serious,and fatality rate was high.So we should pay much attention to acute necrotizing encephalopathy caused by children A H1 N1 influenza.