1.Effect of Short-chain Inulin Supplement on the Gut Microbiota in Mice Fed by High Fat Diet
Lu ZHANG ; Linkang ZHOU ; Rongyu LIAO ; Jiegen WU ; Li XU
Progress in Modern Biomedicine 2017;17(22):4201-4206,4258
Objective:Detect the gut microbiota change in mice caused by 10 % short-chain inulin addition in high fat diet condition.Methods:8-week-old male C57/B6J mice,5 mice were fed with high fat diets,5 mice were fed with high fat diets with 10 % short-chain inulin addition.Fed 8 weeks and then collected fresh feces.Detected the three main short chain fatty acids in fresh feces.Extracted gut bacteria genome DNA for 16S rRNA V4 region sequencing.Principal Coordinate Analysis (PCoA),Alpha diversity and LEfSe analysis were performed to detect gut microbiota changes induced by short chain inulin.Results:Gut bacterial DNA amount and SCFAs amount per gram feces increased.PCoA analysis demonstrated fecal microbiota from inulin and control group mice had distinctive different features and clustered well.Inulin group owned lower fecal microbiota diversity compared with control group.LEfSe analysis revealed that in family level,S24_7 increased,Deferribacteraceae,Lachnospiraceae and Ruminococcaceae decreased.PICRUSt predicted that 22 level 2 KEGG Orthology groups changed.Conclusions:Inulin addition altered the gut microbiota composition in mice in high fat diet condition and impact the gut microbiota gene function.
2.Effect of EP4 gene silencing on the growth and migration of papillary thyroid carcinoma K1 cells
Rongyu ZHONG ; Fen XU ; Heying AI ; Danli ZHOU ; Xiaoying YANG ; Liao SUN
The Journal of Practical Medicine 2018;34(5):702-706
Objective To investigate the effect of EP4 gene silencing on the growth and migration of K1 cells. Methods K1 cells with stable knockdown of EP4 were constructed with lentiviral vector. QRT-PCR and western blot analysis were used to detect the expression of EP4 mRNA and protein in K1 cells. CCK8 assay and flow cytometry were employed to measure cell viability and apoptosis. Transwell assay was applied to detect cell migration. Results Compared with the negative control group,the mRNA and protein expression of EP4 were sig-nificantly decreased in K1 cells with stable knockdown of EP4. Furthermore,shRNA-mediated silencing of EP4 gene remarkably suppressed cell viability and induced apoptosis of K1 cells.The migration of K1 cells with knock-down of EP4 was decreased compared with the negative control group. Conclusions EP4 gene silencing can in-hibit growth and induce apoptosis of K1 cells.Downregulation of EP4 can significantly reduce migration of K1 cells.