Objective To investigate the effects of Krüppel like factor 4 (KLF4)on matrix metalloproteinase 9 (MMP9)ex-pression in hepatocellular carcinoma(HCC).Methods A total of 50 primary hepatocellular carcinoma samples and their correspond-ing adjacent tissues specimens were collected.The expression of KLF4 and MMP9 were detected by IHC,Western blot and qRT-PCR.After KLF4 gene was transfected into hepatocellular carcinoma cell line (HepG2 cell line),the expressions of KLF4 and MMP9 were conformed by qRT-PCR and Western blot.Migration and invasion of HepG2 cell line transfected by KLF4 were detec-ted by wound-healing assay and invasion assay.Results Compared to corresponding adjacent tissues,The expression of KLF4 was significantly lower in HCCs(P <0.05),and MMP9 expression was remarkably higher in HCCs(P <0.05).KLF4 over-expression inhibited the expression of MMP9 on the protein and mRNA levels.Wound-healing assay and invasion assay confirmed that KLF4 regulated cell invasion and migration through regulating MMP9 expression.Conclusion KLF4 showed low expression in HCCs,and MMP9 was overexpressed.Up-regulation of KLF4 could decrease the expression of MMP9 in HepG2 cell line,which inhibited inva-sion and migration.

In 2012, the National Central Cancer Registry (NCCR) of China collected cancer registration information for the year 2009 from local cancer registries and analyzed it to describe the incidences and mortalities of cancers in China. Based on the data quality criteria from NCCR, data from 104 registries covering 85,470,522 people (57,489,009 in urban areas and 27,981,513 in rural areas) were checked and evaluated. The data from 72 registries were qualified and accepted for the cancer registry annual report in 2012. The total cancer incident cases and cancer deaths were 244,366 and 154,310, respectively. The morphologically verified cases accounted for 67.23%, and 3.14% of the incident cases only had information from death certifications. The crude incidence in the Chinese cancer registration areas was 285.91/100,000 (317.97/100,000 in males and 253.09/100,000 in females). The age-standardized rates for incidences based on the Chinese standard population (ASRIC) and the world standard population (ASRIW) were 146.87/100,000 and 191.72/100,000, respectively, with a cumulative incidence of 22.08%. The cancer mortality in the Chinese cancer registration areas was 180.54/100,000 (224.20/100,000 in males and 135.85/100,000 in females). The age-standardized rates for mortalities based on the Chinese standard population(ASRMC) and the world standard population (ASRMW) were 85.06/100,000 and 115.65/100,000, respectively, and the cumulative mortality was 12.94%. Lung cancer, gastric cancer, colorectal cancer, liver cancer, esophageal cancer, pancreatic cancer, encephaloma, lymphoma, female breast cancer, and cervical cancer were the most common cancers, accounting for 75% of all cancer cases. Lung cancer, gastric cancer, liver cancer, esophageal cancer, colorectal cancer, pancreatic cancer, breast cancer, encephaloma, leukemia, and lymphoma accounted for 80% of all cancer deaths. The cancer registration's population coverage has been increasing, and its data quality is improving. As the basis of the cancer control program, the cancer registry plays an important role in directing anticancer strategies in the medium and long term. Because cancer burdens are different in urban and rural areas in China, prevention and control efforts should be based on practical situations.
China ; epidemiology ; Female ; Humans ; Incidence ; Male ; Neoplasms ; epidemiology ; mortality ; Registries

Objective: To estimate the cancer incidence and mortality in Jiangsu, 2013. Methods: Up to June 2016, registration data in 2013 from 35 cancer registries in Jiangsu was collected by Jiangsu Provincial Central Cancer Registry. All data were checked and evaluated by the method and criteria of data quality control making by the National Central Cancer Registry, data from 34 registries' data were eligible and accepted as pooled data. Stratified by urban and rural, gender, age and cancer site, crude incidence/mortality rate, age-specific incidence/mortality rate, age-standardized incidence/mortality rate by Chinese standard population, age-standardized incidence/mortality rate by world standard population, composition, cumulative incidence/mortality rate (0-74 age years old) and the 10 leading cancers were calculated. Cancer incidence and mortality were estimated by using provincial population of Jiangsu in 2013. Chinese population census in 2000 and World Segi's population were used to age-standardize incidence and mortality. Results: All 34 cancer registries (10 in urban and 24 in rural areas) covered a total of 38 938 470 population (19 682 921 in males and 19 255 549 in females), accounted for 51.12% of whole provincial population in 2013. It was estimated that new cancer cases and cancer deaths were 232.6 thousands (136.7 thousands in males and 95.9 thousands in females) and 155.3 thousands (99.7 thousands in males and 55.6 thousands in females), respectively. The crude incidence rate in Jiangsu in 2013 was 305.22/100 000, age-standardized incidence rate by Chinese standard population and by world standard population were 184.04/100 000 and 181.16/100 000, respectively, with cumulative incidence rate (0-74 age years old) of 21.38%. The crude mortality rate was 203.94/100 000, age-standardized mortality rate by Chinese standard population and by world standard population were 116.28/100 000 and 115.12/100 000, respectively, with cumulative mortality rate (0-74 age years old) of 13.11%. Cancers of lung, esophagus, stomach, liver, colorectum and female breast were the most common cancers in Jiangsu, accounting for about 71% of all new cancer cases in 2013. Lung cancer, esophageal cancer, stomach cancer, liver cancer and colorectal cancer were the leading cause of cancer death, accounting for about 74% of all cancer deaths. Conclusion The crude incidence and mortality rate of cancer in Jiangsu Province were at a high level. For the most common cancers such as lung, esophagus, stomach, liver, colorectum and female breast cancer, effective prevention and control activities should be strengthened.