Objective To analyze and evaluate the impact of trusteeship on hospital management and operating status. Methods The state of affairs, technical merit and financial situation and other datawere invested and analyzed in some trust hospital in Xinjiang from 2007 to 2010. Results The number of outpatients, inpatients and surgeries compared before hosting increased by 30. 3%, 61.3% and 26. 3%, respectively. The average in hospital shortened 1.1 days. The growing medical expenditure of average outpatientand inpatient was controlled at a reasonable range. 33 new technologies were carried out after trusteeship. The total revenue in 2010 increased 54.2% compared to 2007, with the balance between revenue and expenditure. Conclusion Hospital trusteeship helped improve hospital operation and management. Measures for hospital trusteeship should be suited to local conditions and sensible.

Objective To investigate the biosynthesis of arbutin by suspension culture of crown gall of transgenic Panax quinquefolium. Methods Hydroquinone in methanol(60 mg/mL) was added to the medium of the crown gall of P.quinquefolium after precultured for 20 d,then they were co-cultured for another 60 h.The product was isolated and purified by column chromatography and its structure was identified by physicochemical properties and spectroscopic data.The dynamic curve of biotransformation was investigated by quantative analysis of arbutin with HPLC.Results The product could be obtained from both of the culture and medium,which was isolated and elucidated as 4-hydroxyphenyl-?-D-glucopyranoside(arbutin).After co-cultured for 60 h,the mole conversion ratio of hydroquinone is 88.4%,the product contents in culture and medium are 63.69 mg/flask and 1.86 mg/flask,respectively,and the excretion ratio of arbutin reaches the highest(2.92%).Conclusion It's the first time around the world that the crown gall of transgenic P.quinquefolium is used as a biotransformation system and arbutin which shows varied pharmacological activites have been got successfully.

Connexin (Cx) is the main component of gap junction channels, which is essential for intercellular communication between adjacent cells.Cx plays an important role in regulation of the growth, differentiation and apoptosis of cells and is closely related to tumor formation and development.The expression of Cx appears to be different in different stages of tumor development, and the regulatory mechanism of Cx is also involved in many aspects.In this paper, recent advances in the studies on Cx are reviewed, including the relationship between Cx and development of tumor, the basic mechanism of Cx, as well as its applications in antitumor-drug screening and tumor therapy.

Object To investigate the application of cell immobilization culture to producing ginkgolides in Ginkgo biloba L. Methods To immobilize the cells by polyaminoresin foam as support materials, and determine the effects of different factors. Results The results showed that 71% of immobilization ratio and 22 mg/cm 3 of cell density were obtained using P29 of high density, small bore diameter with pieces of 0.5 cm ? 0.5 cm ? 0.5 cm, 0.72 g of support matrices per bottle in 65 mL of MS medium solution supplemented with 2,4-D 8.0 mg/L+KT 0.04 mg/L+NAA 0.4 mg/L and the incoulum quantity was 200 g/L. Conclusion It shows that present method has some advantages, such as simpleness, low cost, high immobilization ratio.

Objective To investigate the biotransformation of furannoligularenone by cell suspension cultures of Nicotiana tabacum. Methods Furannoligularenone was added to the medium of the suspension cells of N. tabacum after precultured for 10 d, then they were co-cultured for another 4 d. The biotransformed products were detected with HPLC and isolated by various chromatographic methods. The chemical structures of biotransformed products were elucidated on the basis of their physicochemical pro-perties and spectroscopic data. Otherwise, the influence of co-cultured time on conversion ratio was investigated either. Results The substrate, furannoligularenone (Ⅰ), was successfully biotransformed by N. tabacum cultured cells. Two products, 3-oxo-eremophila-1, 7(11)-dien-12, 8-olide (Ⅱ) and 3-oxo-8-hydroxyeremophila-1, 7(11)-dien-12, 8-olide (Ⅲ) were obtained. After co-cultured for 5 d, the mole conversion ratio of the substrate reached the highest (53.1%). Conclusion It's the first report on biotransformation of furannoligularenone by N. tabacum cultured cells.

ObjectiveTo analyze the potential risk factors for relapse and development of epilepsy in patients with benign in-fantile convulsions associated with mild gastroenteritis (BICE).MethodsA total of 264 cases of BICE were recruited. Accord-ing to the frequency of convulsions, the patients were divided into single group (n=134, convulsion once), and multiple group (n=130, convulsions≥2 times). According to convulsion duration, the patients were divided into short-term group (n=186, con-vulsions duration <5 minutes) , and long-term group (n=78, convulsion duration≥5 minutes). The clinical data obtained during hospitalization and follow-up were analyzed.ResultsIn multiple group, 9.23% were relapsed and 6.15% developed epilepsy. In single group, 2.99% were relapsed and 0.75% developed epilepsy. There were signiifcantly different in the rate of relapses and development of epilepsy between two groups (P<0.05). In the long-term group, 12.82% were relapsed and 8.97% developed epi-lepsy. In the short-term group, 3.23% were relapsed and 1.08% developed epilepsy. There were signiifcantly different in the rate of relapses and development of epilepsy between the two groups (P<0.05).ConclusionsThere are the risks of relapse and development of epilepsy in BICE patients. Convulsions≥ 2 times and≥5 minutes may be the risk factors of relapse and devel-opment of epilepsy.

Objective To study the significance of level changes of serum hydrogen sulfide(H2 S) and interleukin-6(IL-6)in children with hand,foot and mouth disease(HFMD).Methods Nity-two cases with HFMD were enrolled and divided into severe HFMD group(48 cases)and common HFMD group(44 cases).Forty-six healthy children were enrolled as healthy control group.The serum H2 S and IL-6 were detected.Results Acute phase:compared to the healthy control group[H2 S(55.76 ±7.80)μmol /L,IL-6 (61.31 ±13.43)ng /L],the level of serum H2 S significantly reduced and the level of IL-6 significantly increased in severe HFMD group[H2 S(21.72 ±7.52)μmol /L;IL-6(131.33 ±17.82)ng /L]and common HFMD group[H2 S(31.86 ±8.41 )μmol /L;IL-6(95.48 ±15.07)ng /L](P ﹤0.01 ),and there was signifi-cantly difference between the severe HFMD group and common HFMD group(P ﹤0.01 ).Recovery phase:compared to the healthy control group,the serum H2 S level[(34.54 ±13.21 )μmol /L]was lower and IL-6 [(92.73 ±15.25)ng /L]was higher in severe HFMD group(P ﹤0.01 ).The serum H2 S level was negatively correlated with IL-6 in severe HFMD group and common HFMD group(r =-0.31 ,P ﹤0.01 ;r =-0.45, P ﹤0.01 ).Conclusion The serum H2 S and IL-6 participate in the pathological process of HFMD,and the level change can be used as one of indicators of early diagnosis.

Objective To investigate the effects of zoledronic acid on the expression of HIF-1α and VEGF in osteosarcoma LM8 cell line under hypoxic condition. Methods The hypoxic culture model was established. After LM8 cells were treated with zoledronic acid, semi-quantitative PCR was used to assess the expression of HIF-1α and VEGF mRNA. The expression of HIF-lct and VEGF protein was de-tected by immunohistochemical staining and ELISA respectively. Results Compared with cells in normoxic conditions, cells in the hypoxic environment and cells treated with zoledronic acid in the hypoxic condition did not show a significant change in the mRNA level of HIF-1α(P >0. 05). However, the protein expression of HIF-1α was markedly decreased in the cells treated with zoledronic acid in the hypoxic envi-ronment. In contrast, both mRNA and protein expression levels of VEGF were down-regulated in the zoledronic acid treatment hypoxic group (P <0.05). Conclusion Under hypoxic conditions in vitro, zoledronic acid inhibited the expression of HIF-1α protein, which decreased VEGF mRNA level and protein expression in osteosarcorna LM8 cell line.

OBJECTIVETo investigate the influence of 2,3-dihydrocyclopenta coumarins to the growth of transgenic hairy root of Polygonum multiflorum and the accumulation of active ingredient stilbene glucoside in the culture.

METHODIn different culture time, 2,3-dihydrocyclopenta coumarin compounds were administrated to the hairy roots. The yield of stilbene glucoside and the growth of the hairy roots were determined after some period of co-culture. Through the investigation of the different concentrations of the 2,3-dihydrocyclopentacoumarin, and sub-culture times, the optimal experimental conditions were established.

RESULTThe optimal administration time of the 2,3-dihydrocyclopentacoumarin was on the fourth day, the optimal concentration of 2,3-dihydrocyclopentacoumarin was 0.025 g x L(-1). Under this condition, the growth of the hairy root greatly increased. Comparing with control group, the accumulation of stilbene glucoside was increased about four times.

CONCLUSION2, 3-dihydrocyclopentacoumarins could promote the hairy hair root growth and stimulate the stilbene glucoside accumulation.

Coumarins ; pharmacology ; Glucosides ; metabolism ; Plant Roots ; drug effects ; growth & development ; metabolism ; Polygonum ; drug effects ; growth & development ; metabolism ; Stilbenes ; metabolism

Objective To observe the effects of comprehensive treatment of aerobic exercise and medicine on the levels of serum immunoglobulins and cytokines in rats with chronic abacterial prostatitis (CAP).Methods Forty healthy adult male SD rats were randomly divided into the normal control group,CAP model control group,exercise treatment group,medicine treatment group and exercise and medicine comprehensive treatment group,8 cases in each group.The CAP rat model was prepared by the injection of Xiaozhiling,and after 7 d of rat model construction,the exercise treatment group and exercise and medicine comprehensive treat ment group conducted once swimming exercise at fixed time every day,6 d per week for 4 continuous weeks;the medicine treatment group and exercise and medicine comprehensive treatment group were given Qianlieshutong Capsule aqueous solution for gavage treatment every day;the normal control group,model control group and exercise treatment group performed gastric gavage with sterile normal saline.On 14,28 d of the treatment process the rats in each group were sacrificed.The indicators such as serum immunoglobulins and cytokines in rats were detected by using ELISA.Results On 14 d,compared with the normal control group,the levels of serum IgG,IgA,IgM,TNF-α,IL-1β and IL-6 in each experimental group were increased significantly (P＜ 0.05),while which had no statistically significant difference between the exercise treatment group and model control group and between the comprehensive treatment group and medicine treatment group(P＞0.05);on 28 d,the levels of IgG,IgM,TNF-α,IL-1β and IL-6 in the exercise treatment group were significantly lower than those in the model control group(P＜0.05),the levels of IgG,IgA,TNF-α,IL-1β and IL-6 in the comprehensive treatment group were significantly lower than those in the medicine treatment group (P＜0.05),moreover were close to those in the normal control group(P＞0.05).Conclusion Aerobic exercise can reduce the levels of serum immunoglobulins and cytokines in CAP rats,and the effect is more obviousin CAP rats treated by comprehensive therapy combined with medicine.