1.Determination of Bis-(5-formyl-furfuryl)-ether and 2,4-Dihydroxy-6-methoxy-3-methyl-acetophenone in the Root of Yuexiandaji(Euphorbia ebracteolata) by HPLC
Kuijun ZHAO ; Guojun XU ; Rongluan JIN
Chinese Traditional and Herbal Drugs 1994;0(02):-
An HPLC method for the quantitative analysis of bis (5-formyl-furfuryl ) -ether an d 2, 4-dihydroxy-6- methoxy-3-methyl- acetophenone in Chinese drug,Langduc (Euphorbia ebracteolata)was developed. The solvent system used was methanol-water-10% acid (45 : 55 : 2 ) (adjusted to pH 5.4 by 10% ammonia ) on ODS columm at 289nm.Tetrahydro palmatine was used as an internal standard. The operation can be completed in 30min.This method is sensitive,simple and accurate with good reproducibility.It can be applied to the quality control of the crude drug of E. ebracteolata.
2.Variations of Chemical Compositiion of Leech(Scalded)
Lifang LIU ; Rongluan JIN ; Guojun XU ; Zhibing WU ;
Chinese Traditional Patent Medicine 1992;0(12):-
Objective:To provide chemical basis for processing of leech through investigating the variations of composition of leech scalded with talc powder. Methods: Amino acids were determined by HPLC post column derivatization and steroids by GC MS. Results: The results showed the total contents of amino acids in scalded leech were reduced and the steroids of it were changed. Conclusion: The processing method of leech used at the moment has a great influence in chemical components of leech.