Objective: To observe the effect of valsartan and amlodipine on the reduction of blood pressure in aged hyperpietic patients,as well as on the improvement of left ventricular diastolic function,endothelium function and insuline resistence. Methods: We divided 40 cases of aged hypertension patients(1 to 2 grade) into Valsartan and Amlodipine groups at random,giving valsartan 80-160 mg or amlodipine 5-10 mg,respectively,for 12 weeks as a treating period.We measured the blood pressure twice a week,observed the differences of blood pressure and heart rate before and after the treatment,as well as the changes of insuline sensitivity,left ventricular diastolic function and endothelium vascular disatolic function. Results and Conclusion: Both valsartan and amlodipine can effectivly reduce the blood pressure of aged hypertension patients(l to 2 grade).They can also improve the diastolic function,insuline resistance and endothelium dependent vascular diastolic function.

Objective To observe the effectiveness and safety of endovascular stent assisted angioplasty for symptomatic intracranial arteriostenosis.Methods 4 patients with symptomatic intracranial arteriostenosis were treated with endovascular angioplasty using balloon expandable stents. Therapeutic effects were evaluated by clinical manifestations, digital subtraction angiography (DSA) and transcranial Dopple (TCD) before and after the operation. Follow up was made for 7~10 months.Results Technical success was achieved in all patients, with no complication of cerebral embolism and hemorrhage except one of transient seizure during operation. Angiographic results immediately after stenting suggested that the residual postoperation stenosis was 8%(0%~17%), with significant reduction from preoperation stenosis of 75%(70%~80.7%). No recurrent TIA and stroke occurred and no restenosis was found with serial TCD in all 4 patients during follow up of 7~10 months.Conclusions Endovascular stenting is a safe and effective therapeutic tool for symptomatic intracranial arteriostenosis.

Objective To investigate the effectivety and safety of carotid angioplasty and stenting (CAS) with cerebral protection fitler for elder and high risk patients with symptomatic carotid stenosis.Methods 25 patients with symptomatic carotid stenosis over 60 years old at high risk were treated with CAS. Results The procedures were technically successful in all patients. All the degrees of stenosis after CAS were less than 20%. Clinical symptoms disappeared. Heart rate and blood pressure were decreased differently in 24 cases.None of them experienced serious clinical complications. Conclusion CAS with cerebral protection fitler are safe and effective in treatment of elder and high risk of patients with symptomatic carotid stenosis.

Objective To evaluate the immediate results and the 6 month angiographic restenosis rate after repeat percutaneous intervention for in stent restenosis (ISR) Methods The acute and 6 month follow up outcomes of 156 patients with ISR who were treated by repeat PTCA or stenting were analyzed in this retrospective study Quantitative coronary angiography analyses (QCA) were performed before and after repeat intervention for in stent restenosis and on a average 6 month coronary angiogram to assess the recurrent restenosis rate Results All the patients were treated successfully Balloon angioplasty was performed in 134 patients (85 9%) and repeat stenting in 22 patients (14 1%) The six month follow up coronary angiogram showed the recurrent restenosis rate was 24 3% The recurrent restenosis rate was higher in 40 diffuse ISR lesions than that in 96 focal ISR lesions: 45% vs 18%, P75% was also the risk factor of recurrent restenosis Conclusion For the most patients with ISR, repeat PTCA or intracoronary stenting seems to be an effective and safe method The overall restenosis rate after repeat intervention for ISR is the same as that of initial intracoronary stenting

Objective To analyze the effect and safety of cerebral protection filter in carotid artery and stenting(CAS) for the treatment of severe symptomatic carotid artery stenosis.Methods Elective CAS with the use of cerebral protection filters were undergone in 26 consecutive patients with severe carotid artery stenosis.The filter devices were advanced through lesions and opened distal to the lesions followed by CAS.The incidence of cerebral ischemic events and the debris collected in the filters were observed and analyzed.Results In all the patients,it was feasible and successful to position the filters devices in the internal carotid artery.After the filter devices were positioned,CAS were performed successfully in all the 26 patients.Thrombus or plague debris were found in 14 out of 26 retrieved filters(54%).There was no death or symptomatic cerebral thromboembolic event during the CAS procedure.Asymptomatic cerebral embolization lesions were detected in 3 patients by the means of diffusion-weighted magnetic resonance imaging after 1-6 days of the CAS.No major cerebral or cardiovascular ischemic events were recorded in all patients during their follow up period of 6-12 months.Conclusion Carotid artery stenting is an effective microinvasive therapy for severe carotid artery stenosis which effect and safety can be enhanced with the use of cerebral protection filter.

Objective To study the glutamine combined with early enteral nutrition’s effects on severe acute pancreatitis’systemic inlfammation. Methods 150 Cases with severe acute pancreatitis were divided into total parenteral nutrtion group(TPN group), early enteral nutrition group(EEN group) and Glutamine and early enteral nutrition group(G+EEN group) according to their therapeutic methods. The curative effects, APACHEⅡscore and liver and kidney function were compared after treatment. Inlfammatory cytokines of hs-CRP, TNF-α, IL-1β, IL-6, IL-8 and IL-10 before and after treatment were detected and compared. Results The efifciency rate in G+EEN group was signiifcantly better than that in TPN and EEN group(P<0.05), with lower APACHEⅡscore and better liver and kidney function. The level of hs-CRP, TNF-α, IL-1β, II-6 and IL-8 after treatment in G+EEN group were signiifcantly lower than that in TPN and EEN group(P<0.05), except IL-10, which was signiifcantly higher than that in TPN and EEN group (P<0.05). Conclusion Glutamine combined with early enteral nutrition could signiifcantly ameliorate severe acute pancreatitis’systemic inlfammation, its curative effects is better than early enteral nutrition and total parenteral nutrition.

Anticoagulants ; adverse effects ; pharmacology ; Blood Coagulation Disorders ; chemically induced ; Drug Interactions ; Humans ; Warfarin ; adverse effects ; pharmacology

We studied the regulatory effects of the estragen receptorbeta (ERbeta) gene on the downstream estrogen signal transfection pathway in colon cancer cells and the possible mechanisms involved. A human ERbeta gene recombinant expression plasmid, pEGFP-C1-ERbeta, was constructed and transfected into the Caco-2 colon cancer cell line, a line with low ERbeta gene expression. The expression of ERbeta mRNA and protein was detected 72 h after transfection. RT-PCR was used to examine the expression levels of the progesterone recepror (PR) gene containing the classic estrogen response element (ERE), the C-fos oncogene containing the AP-1 site (a non-classical ER binding site), the epigenetic modifying genes, such as Dnmt1, Dnmt3a, Dnmt3b, and histone methyltransferase (HMT), and the human mismatch repair gene hMLH1. Methylation-specific PCR was used to detect the changes in the methylated sites of the CpG islands in the promoters of the ERbeta, PR, and C-fos genes. The results indicated that the human ERbeta gene recombinant expression plasmid pEGFP-C1-ERbeta was successfully constructed and transfected into Caco-2 cells. As compared with the control group, the mRNA and protein expression of ERbeta gene was increased significantly 72 h after the transfection of pEGFP-C1-ERbeta into the Caco-2 cells. As compared with the control group, the mRNA expression of the PR, C-fos, Dnmt3a and Dnmt3b genes was increased significantly 72 h after the transfection of pEGFP-C1-ERbeta into the Caco-2 cells, but the mRNA expression of the Dnmt1, HMT, and hMLH1 genes decreased significantly (P<0.05). As compared with the control group, different degrees of demethylation occurred in the promoters of the ERbeta, progesterone receptor (PR), and C-fos oncogene 72 h after the transfection of pEGFP-C1-ERbeta into the Caco-2 cells. The methylation index of the estrogen signal transfection pathway in Caco-2 cells was decreased significantly following the expression restoration of ERbeta gene (P<0.05). It is concluded that the restoration or up-regulation of the ERbeta gene in Caco-2 cells may significantly activate the expression of the related target genes in the downstream estrogen signal transfection pathway and may result in the demethylation changes of the pathway. During the process, the expression level and activity of the epigenetic modifying genes and the human mismatch repair gene have changed simultaneously. The regulatory effect of the ERbeta gene on the estrogen signal transfection pathway to a certain extent partly involves demethylation.

Objective: To investigate the neural connections between Shenmen (HT7)-heart and the brain by observing the tracing viruses co-labeled brain nuclear groups after injection of the pseudorabies viruses (PRV), the reverse transsynaptic virus tracer carrying different fluorescent protein genes, into the myocardium and Shenmen (HT7) point, respectively.Methods: Pseudorabies virus 531 (PRV531) carrying the green fluorescent protein gene and pseudorabies virus 724 (PRV724) carrying the red fluorescent protein gene were injected into the left ventricular wall and Shenmen (HT7) point area of the left forelimb of six C57BL/6 mice, respectively. After 120 h, whole brain tissue was extracted under 4％ paraformaldehyde perfusion to prepare brain sections. Neuronal co-labeling with the tracing viruses was observed under fluorescence microscopy. Results: Co-labeled signals from the mouse ventricular wall and Shenmen (HT7) point region were found at all levels of the mouse central nervous areas, such as the cerebral cortex, hypothalamus, midbrain, pons, and medulla oblongata. The number of co-labeled neurons was higher in the primary motor area, the hypothalamic paraventricular nucleus, the subceruleus nucleus, and the paramedian reticular nucleus. Conclusion: There is a neural connection between Shenmen (HT7), the heart, and the brain, which may be most closely related to the autonomic nervous system.

Objective To evaluate the efficiency of glycogen phosphorylase BB (GPBB) and heart-type fatty acid binding protein (H-FABP) in the early diagnosis of neonatal myocardial injury (NMI). Methods The GPBB and H-FABP levels in the blood were detected at 3, 6, 12 and 24 h after onset of the NMI. The GPBB and H-FABP levels were compared among NMI, Non-NMI and control groups. The NMI diagnosis sensitivity and specificity for GPBB, H-FABP, GPBB combined with H-FABP were compared. The relationships of GPBB, H-FABP levels with the risk factors were analyzed. Results The GPBB and H-FABP levels of NMI group were higher than those of Non-NMI and control groups (P < 0.01). The diagnosis sensitivity of four time points (3, 6, 12 and 24 h) of combined GPBB and H-FABP were higher than those of the GPBB and H-FABP (P < 0.05). The GPBB and H-FABP were negatively correlated with blood glucose level, positively correlated with asphyxia degree and septicemia degree. Conclusion The combined application of GPBB and H-FABP can improve sensitivity in the early diagnosis of NMI.