1.Expression of MRP1 and CD34 in colorectal carcinoma tissue and its clinical significance
Wenzeng ZHAO ; Ronghong LIU ; Jianbing WANG ; Rong QI ; Hongmei XIE ; Lixian ZHANG ; Shunmao MA ; Bin CAO
Chinese Journal of Primary Medicine and Pharmacy 2012;19(20):3041-3043
ObjectiveTo explore the expression of MRP1 and CD34 in colorectal carcinoma tissue and the relationship with clinicopathological factors.MethodsImmunohistochemical streptavidin-perosidase method was used to examine the expression of MRP1 and CD34 in 53 cases with colorectal carcinoma and normal colorectal tissue.The correlation between the levels of MRP1and CD34 expression and clinicopathological factors were analyzed.ResultsThe positive expression rates of MRP1 in the carcinoma group and normal colorectal tissue group were 49.1% and 15.1% respectively,and there was a significant difference of the positive expression between the two groups( x2 =14.029,P < 0.01 ).The expression of MRP1 had no correlation with the degree of differentiation,the depth of invasion,the metastasis of lymph node and all the other clinicopathological factors ( P > 0.05 ).CD34 value in the carcinoma group and normal colorectal tissue group were ( 35.63 ± 12.23 ) MVD/HP and ( 6.12 ± 0.97) MVD/HP,respectively,and there was a significant difference between the two groups (t =17.565,P < 0.01 ).CD34 was not correlated with age,sex,tumor size,localization of the primary tumor ( P > 0.05 ),but correlated with Dukes staging,lymph node metastasis,differentiation of the tumor,depth of invasion( all P < 0.05).ConclusionThe overexpression of MRP1 and CD34 protein may involve in colorectal carcinogenesis;MRP1 may involve in the primary multidrug resistance in colorectal carcinoma.; CD34 may involve in the colorectal carcinoma invasion and metastasis.Investigating the expression of MRP1 and CD34 in colorectal carcinoma simultaneously can provide new referential indexes for the treatment and prognosis of colorectal carcinoma.
2.Mouse-adapted SARS-CoV-2 replicates efficiently in the upper and lower respiratory tract of BALB/c and C57BL/6J mice.
Jinliang WANG ; Lei SHUAI ; Chong WANG ; Renqiang LIU ; Xijun HE ; Xianfeng ZHANG ; Ziruo SUN ; Dan SHAN ; Jinying GE ; Xijun WANG ; Ronghong HUA ; Gongxun ZHONG ; Zhiyuan WEN ; Zhigao BU
Protein & Cell 2020;11(10):776-782
Adaptation, Physiological
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Adenosine Monophosphate
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administration & dosage
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analogs & derivatives
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pharmacology
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therapeutic use
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Administration, Intranasal
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Alanine
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administration & dosage
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analogs & derivatives
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pharmacology
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therapeutic use
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Animals
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Betacoronavirus
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genetics
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physiology
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Chlorocebus aethiops
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Coronavirus Infections
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drug therapy
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virology
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Disease Models, Animal
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Female
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Host Specificity
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genetics
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Lung
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pathology
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virology
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Male
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mutation, Missense
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Nasal Mucosa
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virology
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Pandemics
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Pneumonia, Viral
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drug therapy
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virology
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RNA, Viral
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administration & dosage
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genetics
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Turbinates
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virology
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Vero Cells
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Viral Load
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Virus Replication