Objective To study the effects of ethanol extract from Java Brucea Fruit on platelet-derived growth factor receptor ?(PDGFR?) mediated cell migration and to obtain the valuable messages on the characteristics of its active ingredient.Methods PAE cells was transfected with the vector expressing human PDGFR? with Transfectom 2000;After screening by G418 resisitance,RT-PCR was used to monitor the expression of PDGFR? in the cells;Wound healing of the cells was used to examine the lowest consistency of PDGFBB and inhibitory effect of ethanol extract of Java Brucea Fruit on cell migration after restoring 24 h.Results Human PDGFR? was stably expressed in PAE cells transfected with the expressing vector.The lowest consistency of exogenous PDGFBB which promoted PDGFR? mediated cell migration was 10 ?g?L-1.70% ethanol extract of Java Brucea Fruit which strongly inhibited PDGFR? mediated cell migration was dose-dependent(P10 ?g?L-1) mainly caused the death of the cell.Conclusion Ethanol extract of Java Brucea Fruit has a strongly inhibitory effect on the PDGFR? mediated cell migration which could play a major role in its effects against metastasis of malignant tumor,the active ingredients of it could be more dissolvable in the 70% ethanol.

Objective To study antioxidant activity of extracts from Perinereis aibuhite.Meth- ods The extracts from Perinereis aibuhite were purified by using Sephadex LH-20 and its an- tioxidant activities were determined by the method of DPPH.Results and Conclusion The ex- tracts from Perinereis aibuhite showed strong free radical scavenging activity,and it was identified as a group of low molecular weight peptides by qualitative analyzing method.

Objective To prepare active components from extract of Arca subcrenata Lischke and analyze its antioxidative activity.Methods The antioxidative component(P3)from Arca subcrenata Lischke was isolated by chromatography on Superdex-75 column followed by a Sephadex LH-20 column,and antioxidative activities were assayed using potassium ferricyanide and DPPH methods,respectively.The characters of this component were determined by ninhydrin reagent,anthrone reagent,Coomassie Brilliant Blue G250 reagent as well as thin-layer chromatography.Results and Conclusion A component with strong antioxidative activity was isolated and identified as glycosylated peptide.

Perilla frutescens callus were induced from leave explants on MS medium suplemented with NAA and 2,4-D. The Rosmarinic acid (RosA) content of dried callus was 0.85%. The RosA was extracted from the callus with 80% alcohol and purified through extraction with ethyl acetate and a Sephadex LH-20 column chromatography. The parity of the final product was 95% as analyzed by HPLC RosA could inhibit the growths of Escherichia colt, Staphylococcus aueris and Rhizotonia with MICs of 300, 400, 800?g/mL, respectively.

Objective To develop a high-definition video display system for medical endoscope based on system on chip (SoC),and meet the requirement of high-resolution and real-time video display.Method A CMOS camera was used for video data capture.A SoC chip,integrated with a dual-core ARM Crotex-A9 processor and a field programmable gate array (FPGA),was employed as the kernel of the system.The HPS part of the SoC was used to build an embedded system to realize human-computer interaction,and the FPGA part was used to store and cache video data.The HPS and FPGA part were connected through a high-performance ARM AMBA AXI bus bridge broadband system,so as to achieve encoding of the cached video data and real-time display on screen.Results A high-definition video display system was built based on SoC.This system can achieve capture,processing and realtime display of high-definition video,as well as video freeze function.Conclusions The experimental results indicate that this system is feasible and effective,and possesses the advantages of customizability,multiple-exploitation and high performance of real-time video display.

Objective To study an image display method for portable medical endoscopes using intelligent mobile devices for solving the problem of lacking medical resources in remote areas and carrying inconvenience of endoscopic workstations.Method An high-definition camera was employed for image data acquisition,which was driven by the built-in Video4Linux drive program of a embedded Linux system.A wireless network card was used to establish a point-to-point network,and to build the LAN server.The mobile devices could acquire the collected image data through accessing the local area network,which could realize the real-time display on the LCD screen.Results The resolution of a collected image was 1 024×768 pixels,the bit depth was 24 bits,the frame rate was 30 frame/s,and the actual average transmission speed was about 2 MB/s.Conclusions The proposed method is effective and feasible.The collected image is clear,and the system has advantages of simple structure,low cost and easy to carry,which can save the manufacturing cost and meet the conditions of usage in remote areas.

Spinosyns are secondary metabolites from the aerobic fermentation of saccharopolyspora spinosa Spinosyn is a kind of broad spectrum insecticide, which has contact and ingestion toxicity on insects On lepidopteran insects, spinosad (a mixture of spinosyn A and spinosyn D) is one of the most selective compounds ever discovered In this paper, the structure, biosynthesis, property and process of spinosyns are reviewed

Objective To investigate dynamic changes and clinical significance of the high-sensitive Creactive protein(hs-CRP) level in acute cerebral infarction patients with different etiological types.Methods 136 patients with acute cerebral infarction were recruited.These patients were classified into five subtypes based on Trial of Org 10172 in Acute Stroke Treatment(TOAST) criteria.Serum hs-CRP levels on the 1st,3rd,5th,7th,14th day after onset from patients and 42 healthy controls were measured with immunoturbidimetry.The neurologic impairmentscore was determined with NIHSS.Results Serum hs-CRP levels is higher on the 1th,3rd,5th,7th,14th day than that of the control group ( (4.26 ± 1.31 ),( 12.57 ± 6.29 ),( 10.23 ± 4.49 ),(7.54 ± 2.33 ),(4.25 ± 1.77) mg/Land (2.56 ± 0.86) mg/L,t = 7.89,10.26,10.99,13.55,5.97,P ＜ 0.05 ).Among 5 subtypes,serum hs-CRP was the highest in large-artery atherosclerosis group after acute ischemic stroke,and cardioembolism group was the next.Serum hs-CRP reached the highest on three or five days after disease onset and decreased slowly.High levels of hs-CRP in large-artery atherosclerosis group indicated severe neurologic functional impairment and worsen prognosis.Conclusions ACI is closely related to serum hs-CRP level,which can be used as an subjective index for severity and prognosis with the lasting,high levels of hs-CRP levels predict poor prognosis.

Objective:The roles of HMGA2 in maintaining malignant phenotypes of the osteosarcoma U2OS cells was studied to explore the possibilities for it to be developed as a target for gene therapy.Methods:U2OS cells were stably transfected with a DNA based shRNA expression vector which targeted to HMGA2.The expression of HMGA2 mRNA was proved by RT-PCR;Cell growth,migration and apoptosis were determined with CCK8,hoechst33342 staining and Boyden ventricle,respectively.The mRNA levels of Caspase 3 and Caspase 9 were determined by real time quantitative RT-PCR.Results:The transfection with shRNA expression vector significantly decreased HMGA2 mRNA levels of U2OS cells.Cell growth and migration were decreased,but apoptosis and the mRNA levels of Caspase 3 and Caspas 9 were increased following the decrease of HMGA2 mRNA.Conclusion:The abnormal expression of HMGA2 plays an important role in maintaining the malignant phenotypes of U2OS cells.Gene therapy targeted to HMGA2 could be helpful in the treatment of human osteosarcoma.

Objective To express and purify recombinant protease with thrombolysis activity of Perinereis aibuhitensis Grube and study on its characterization.Methods pMAL-PPA was in- troduced into E.coli DH5?to construct engineering bacteria and overexpression of the prote- ase of fused with maltose binding protein(MBP-PPA)was achieved with IPTG induction. The fusion protein was purified by affinity chromatography on amylose-resin column fol- lowed by chromatography on a DEAE-sepharose FF column.PPA cut with Factor Xa was assayed using casein plates supplied with plasminogen.Results Engineering bacteria express- ing maltose binding protein-thrombolytic protease of P.aibuhitensis were constructed and overexpression of MBP-PPA was achieved with IPTG induction.A recombinant fusion pro- tein of 51kD was purified,and PPA cut down from the fusion protein had a plasminogen-acti- rating activity.The protease showed a good thermal stability with an optimal pH of 8.0. This enzyme was also relatively stable in a pH range of 6.0～9.0 and still active after stored in organic solvents for 20d.Conclusion PPA was verified as a plasminogen activator,and might be a new thrombolytic medicine in the future.