Objective To explore the essential of cerebroprotein hydrolysate injection in the manage-ment of epidemic encephalitis B.MethodsTwo hundred and thirty-two patients with epidemic ence-phalitis B were randomly divided into two groups,one hundred and twenty-six patients in treating group,and one hundred and six patients in controlled group.Beside the conventional and symptomatic therapy,the patients in treating group received the management of cerebroprotein hydrolysate injection 10 mL+5% glucose solution 250 mL,iv drip,qd for 5 d and those in controlled group received the management of interferon?1b 1 MIU,sc,qd for 5 d.ResultsThe total clinical effective rates in treating group and controlled group were 94.6% and 82.6% respectively,there were remark-ably differences between two groups(P

Objective To observe the effects of total paracentesis with normal saline intravenous infusion on portal hemodynamics in refractory ascites of liver cirrhosis.Methods One hundred and forty-two cirrhotic patients with tense ascites were divided into two groups at random.Eighty-seven patients (group A) were treated by total paracentesis with normal saline intravenous infusion beside conventional therapy and diuretics;55 patients (group B) were treated with conventional therapy and diuretics.Results The total effective rate in ascites was 67.8% in group A and 23.6% in group B,P

This guidelines summarize and evaluate all available evidence-at the time of the writing process-on a particular issue , with the aim of assisting physicians in selecting the best management strategies for an individual pa-tient with given condition , taking into account the impact on outcome , as well as the risk-benefit ratio of particular diagnostic or therapeutic means. Guidelines and recommendations should help physicians to make decisions in their daily practice. However , the final decisions concerning an individual patient must be made by the responsible physi-cian(s). A great number of guidelines have been issued in recent years by the European Society of Cardiology (ESC) , as well as by other societies and organizations. ESC guidelines represent the official position of the ESC on a given topic and are regularly updated.

Objective Dilated cardiomyopathy (DCM) is generally considered to be accompanied by both left and right ventricular dysfunction,but most studies only analyze the left ventricular function. In this study, we evaluated the effect of arotinolol on right ventricular function in patients with DCM. Methods Right ventricular ejection fraction (RVEF) and right ventricular diameter (RVD) were measured by two-dimensional echocardiography (2-DE) in 33 DCM patients; RVEF measured by first-pass radionuclide angiography (FPRA) was compared with that by 2-DE. Results The treatment with arotinolol for one year resulted in a reduction in the right ventricular diameter (baseline, 23.0 ± 8.3 mm vs after one-year treatment, 20.7 ± 5.4 mm; P=0.004 ) and an associated increase in ejection fraction (baseline, 36.9 ± 10.3% vs after one-year treatment, 45.8 ± 9.6%; P ＜ 0.001 ); there is a high correlation between the 2-DE method and radionuclide ventriculographic method. The correlation coefficient is 0.933 (P＜0.001). Conclusion Arotinolol therapy could not only improve left ventricular function, but also improve right ventricular function in DCM patients.

Objective: To evaluate the role of histologicalpathology in the diagnosis of periprosthetic joint infection. Methods: A total of 145 cases of joint arthroplasty during October 2017 and October 2018 from Beijing Jishuitan Hospital were collected. There were 23 cases of infection, including knee joint arthroplasty (12 cases) and hip arthroplasty (11 cases). There were 17 females and 6 males. Patients′ age ranged from 39 to 76 years (mean 63 years). The infection was diagnosed if there were >5 neutrophils per high power field in at least 5 high power field. The permanent sections were examined twice separately by two pathologists, and the interval time of histologic examination was at least two weeks. Sensitivity (SE), specificity (SP), positive predictivevalue (PPV), and negative predictive value (NPV) were calculated. The consistency evaluation of histologic examination of two pathologists was calculated by Kappa analysis. Results: The neutrophil cells could locate scattered or focally in the synovium tissue of periprosthetic joint infection. Somewhere, the infiltration of vessel and the perivascular distribution could also exist. Opportunity coincidence rate between two pathologists was 91.3% (Kappa=0.817). The results showed that SE was 60.9%, SP was 100.0%, NPV was 93.1%, PPV was 100.0%. Conclusions The presence of polymorphonuclear cells in histologic examination is correlated with infection. There was high consistency between histologic examination and clinical diagnosis of joint arthroplasty.

Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins. Here, we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla var. yunnanensis. Among them, a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides, respectively. These UDP-glycosyltransferases showed astonishing substrate promiscuity, resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds. A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments, and a mutant UGT91AH1^Y187A with increased catalytic efficiency was obtained. The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes, and 2'-O-rhamnosylation appeared to correlate with strong antifungal effects. The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents.