Objective To establish a method for content determination of Buddleoside in Compound Yinpujiedu Tablet. Method HPLC method, Agilent Eclipse XDB-C18 column (4.6 mm?250 mm, 5 ?m) was used, mobile phase was methanol-4% glacial acetic acid (47∶53), and the detecting wavelength was set at 334 nm. Result The standard curve was linear within the range of 2.24～44.8 ?g. The average recovery and RSD were 97.8% and 1.52%, respectively. Conclusion This method is accurate, reliable, and can provide a scientic index for quality control of Compound Yinpujiedu Tablet.

Objective? ?To?analyze?the?risk?factors?of?delirium?in?patients?in?cardiac?surgery?intensive?care?unit?(CSICU).? Methods? A?prospective?observational?study?was?performed.?Patients?admitted?to?CSICU?of?Fujian?Medical?University?Union?Hospital?from?March?to?August?in?2017?were?enrolled.?The?combination?of?the?Richmond?agitation?sedation?scale?(RASS)?and?the?ICU-confusion?assessment?method?(CAM-ICU)?were?used?to?evaluate?delirium.?The?patient?was?assessed?on?the?second?day?after?CSICU?admission,?twice?a?day,?the?evaluation?was?stopped,?and?the?follow-up??observation?was?terminated?after?the?patient?was?discharged?from?CSICU.?The?patients?were?divided?into?two?groups?according?to?whether?delirium?occurred?in?CSICU.?The?general?and?clinical?treatment?data?(including?condition,?operation,?anesthesia?and?CSICU?treatment)?of?the?two?groups?were?compared.?The?related?factors?of?delirium?were?identified?by?univariate?analysis?and?multifactor?Logistic?regression?analysis.? Results? A?total?of?318?cases?were?included?in?this?study.?Among?them,?93?cases?had?delirium?and?the?incidence?of?delirium?was?29.2%.?It?was?shown?by?univariate?analysis?that?age,?history?of?hypertension,?type?of?surgery,?surgical?procedure,?American?Society?of?Anesthesiologists?(ASA)?anesthesia?classification,?usage?of?propofol,?plasma?transfusion,?red?blood?cells,?platelet?transfusion,?blood?loss,?operative?time,?cardiopulmonary?bypass?(CPB)?time,?myocardial?block?time,?acute?physiology?and?chronic?health?evaluation?Ⅱ?(APACHEⅡ),?duration?of?mechanical?ventilation,?the?length?of?intensive?care?unit?(ICU)?stay,?postoperative?usage?of?diazepam,?midazolam,?fentanyl,?morphine,?chlorpromazine,?etc.?which?were?related?to?delirium,?and?occupation?? (on-the-job?or?self-employed),?medical?insurance?(city?or?provincial?medical?insurance),?education?(primary?to?junior?high?school,?high?school?or?above)?could?reduce?the?risk?of?delirium.?Colinearity?diagnosis?was?performed?on?variables?with?statistically?significant?differences,?and?variables?with?variance?expansion?factor?(VIF)?

Objective To explore the application effect of SCD EXPRESS pressure system in preventing postoperative deep venous (DVT) thrombosis in patients with malignant tumors.Methods Management group of DVT prevention was founded.The operative procedure of SCD EXPRESS pressure system was developed and all people received training.120 patients in Nantong Tumor Hospital from June 2014 to June 2015 were selected and were assigned to the observation group and the control group according the order of admission,with 60 cases in each group.Patients in the control group received routine preventive nursing measures,while patients in the observation group received SCD EXPRESS pressure system to prevent DVT based on the routine measures.The incidence rate of DVT,the time that treatment and care spend,daily cost,average hospitalization time and patient satisfaction degree were compared between two groups.Results One week after surgery,in the intervention group,the incidence rate of DVT was 3.3％,and it was 15.0％ in the control group (x2=4.22,P＜ 0.05).In the intervention group,the time that treatment and care spend was (6.73± 1.04) h and the average hospitalization time was (10.71±1.05) d;while in the control group,they were [(15.57±2.52) h,(17.00± 1.80) d].The satisfaction degree in the intervention group was (93.50±5.50) points and it was higher than that in the control group (84.90 ± 6.40) points (t =17.761,17.876,5.600;P ＜ 0.05).The daily cost in the intervention group was (624.38±85.21) yuan,and it was (593.62±71.74) yuan in the control group (t =-1.76,P＞ 0.05).Conclusions SCD EXPRESS pressure system can effectively prevent the formation of postoperative DVT in patients with malignant tumors;save the time that treatment and nursing spend;shorten the average confinement;improve patient satisfaction;and will not increase the daily cost of treatment.

Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins. Here, we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla var. yunnanensis. Among them, a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides, respectively. These UDP-glycosyltransferases showed astonishing substrate promiscuity, resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds. A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments, and a mutant UGT91AH1^Y187A with increased catalytic efficiency was obtained. The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes, and 2'-O-rhamnosylation appeared to correlate with strong antifungal effects. The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents.