1.VGCC expression in facial nucleus motoneurons after facial nerve injury in adult rats
Lili CAO ; Rongcheng HU ; Zhenggen PIAO
Journal of Practical Stomatology 2017;33(2):162-167
Objective:To investigate the expression change of voltage-gated calcium channels(VGCC) in the facial nucleus motoneurons of adult rats after facial nerve injury.Methods:The facial motor nucleus was localized by retrograde labeling with a fluorescent dye,Dil,and identified by Nissl staining.The facial nerve injury model was established by amputation of the main trunk of left facial nerve.Exposure of the right facial nerve without nerve transection was used as the control.Rats were sacrificed at 3,7,14 and 28 days after injury respectively(n =10),the brainstem containing facial nucleus were collected,the expression of P/Q,N,L,R-type calcium channel α1A,α1B,α1C and α1E subunits was examined by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR).Results:Immunohistochemistry results show that whereas α1A,α1E subunits levels did not vary compared with control group 3,7,14,28 days after injury(P >0.05),α1B and α1C subunits immunoreactivity decreased in the motoneurons after injury,a sharp decrease was detected at 14 days after injury(P < 0.01),thereafter returned to the control level at 28 days after axotomy(P >0.05).The expression of α1B and α1C mRNA was down-regulated,especially 14 days after the injury(P <0.01),and then recovered to normal level at 28 days (P > 0.05).In addition,there was no significant difference of α1A and α1E subunits and their correspoding mRNA between operated group and control group at all time points(P > 0.05).Conclusion:VGCC is involved in facial nerve injury and down-regulation of N-type and L-type calcium channels may be one of the role.
2.Compliance and influence factors of standardized medication in patients with coronary artery disease
Yongcang HU ; Jianhua ZHANG ; Jiacai WANG ; Rongcheng LI ; Chao DING ; Yanyan CHEN ; Xiannan LI ; Yan XU
Chinese Journal of Primary Medicine and Pharmacy 2015;(10):1441-1443
Objective To evaluate the standardized drug treatment and its influence factors of patients,with coronary artery disease,in hospital and one year after discharge.Methods The study enrolled sequentially 165 patients who were firstly diagnosed of coronary artery disease,61 cases with stable angina,67 cases with unstable angi-na and 37 cases of acute myocardial infarction,by coronary artery angiography from 2010 to 2012.The standardized drug treatment and its influence factors of patients were analyzed at hospital and 1 year after discharge in the present study.Results Fifty five percent patients with coronary artery disease at hospital regularly took the four drugs,anti-platelet agents,statins,beta blockers and angiotensin converting enzyme inhibitors/angiotensin II receptor antagonist. The ratio decreased to forty five percent one year after discharge(χ2 =81.04,P <0.01).The reasons of the irregular medication taken were optional withdrawal(61%),following the doctors′advice(15%),economic hardship(20%) and the drugs′adverse reaction(4%).Conclusion The results of the present study showed that the rate of regular medication in patients with coronary artery disease is low in hospital and 1 year after discharge.The major reasons of the irregular medicine taken were the patients′optional withdrawal and the consciousness of second prevention was lack in doctors in our hospital.Therefore,the increase of the consciousness of regular standardized treatment in prima-ry care physicians and strengthen the management of the patients after discharge form hospital.
3.Correlation Study Between Blood Level of Big Endothelin-1 and Cardiac Remodeling in Patients With Hypertrophic Obstructive Cardiomyopathy
Limin LIU ; Rongcheng ZHANG ; Weixian YANG ; Rong LIU ; Jiansong YUAN ; Fenghuan HU ; Jia LI ; Fujian DUAN ; Shengwen LIU ; Changlin ZHANG ; Shubin QIAO
Chinese Circulation Journal 2017;32(1):58-62
Objective:To explore the relationship between big endothelin-1 (big ET-1) and cardiac remodeling in patients with hypertrophic obstructive cardiomyopathy (HOCM).
Methods:A total of 167 consecutive HOCM patients admitted in our hospital from 2015-01 to 2016-05 were enrolled. Blood levels of big ET-1 and NT-proBNP were measured;electrocardiogram(ECG), dynamicECG,echocardiography (UCG) and cardiovascular magnetic resonance (CMR) were examined for relevant statistical analysis.
Results:In all 167 HOCM patients, blood level of Big ET-1 was positively related to NT pro-BNP (r=0.35, P=0.000), left atrial (LA) diameter (r=0.169, P=0.019) and heart rate (r=0.141, P=0.037);negatively related to hemoglobin (r=-0.173, P=0.013) and the ratio of interventricular septum (IVS)/posterior wall of left ventricle (r=-0.165, P=0.017). Based on the finding of positive correlation between Big ET-1 and LA diameter, the patients were divided into 2 groups:Normal LA group, n=74 and Enlarged LA group, n=93. Compared with Normal LA group,Enlarged LA group had the higher blood level of Big ET-1 (P=0.001);increased left ventricular end-diastolic diameter (LVEDD) (P=0.024), thicker IVS (P=0.003), lower LVEF (P=0.001);enlarged LVED volume (P=0.002) and IVS (P=0.002);elevated ratio of atrial fibrillation(AF);more patients with moderate to severe mitral regurgitation (MR) and NYHA functional classIII/IV.
Conclusion: Blood level of Big ET-1 was positively related to NT pro-BNP and LA diameter in HOCM patients;the patients with enlarged LA had increased Big ET-1, more obvious cardiac remodeling and the higher incidence of AF occurrence which implied that Big ET-1 might play the role in cardiac remodeling in HOCM patients.
4.Application of Child-Turcotte-Pugh Scores in Predicting the Risk of Death for In-hospital Heart Failure Patients
Xuemei ZHAO ; Yuhui ZHANG ; Rongcheng ZHANG ; Yan HUANG ; Yiran HU ; Xiaoning LIU ; Mei ZHAI ; Yunhong WANG ; Tao AN ; Tianyi GAN ; Jian ZHANG
Chinese Circulation Journal 2016;31(7):668-672
Objective: Heart failure (HF) patients are usually associated with liver function impairment, Child-Turcotte-Pugh (CTP) scores can evaluate liver function, but its effect in HF patients has been unclear. We want to study the application of CTP scores in predicting the risk of death for in-hospital HF patients. Methods: A total of 1180 consecutive in-hospital HF patients were enrolled. According to CTP scores evaluated liver function at admission, the patients were divided into 3 groups: CTP grade A group, n=951, CTP grade B group, n=206 and CTP grade C group, n=23. The endpoint of this study was all-cause death. Results: There were 180 patients died at 1 year follow-up period, the in-hospital and 1 year mortalities were increased with the elevated CTP grades accordingly: for in-hospital mortalities in CTP grade A, B and C groups were (0.8%, 11.7% and 56.5%) respectively, P< 0.001; for 1 year mortalities were (9.6%, 34.5% and 78.3%) respectively, P< 0.001. Multivariable Cox regression analyses indicated that the higher CTP grades, the higher risk of in-hospital and 1 year mortalities in HF patients. The area under curve for CTP scores in predicting the in-hospital and 1 year mortalities were 0.88 and 0.74 respectively. Kaplan-Meier survival analysis presented that the patients with improved CTP scores from grade B or C to grade A at discharge had the higher 1 year survival rate than those without improvement, P=0.028.
Conclusion: CTP scores may independently predict the risk of death for in-hospital HF patients, the levels of CTP scores might be used for evaluating the efficacy of in-hospital treatment.
5. Root canal anatomy of maxillary second premolars at various ages observed by cone-beam CT
Rongcheng HU ; Wei XIE ; Yaqun HU ; Zhenggen PIAO
Chinese Journal of Stomatology 2019;54(11):733-738
Objective:
To observe the morphological changes of root canals in maxillary second premolars at various ages by using cone-beam CT (CBCT) in order to provide imaging and theoretical reference for clinical treatments.
Methods:
The digital CBCT data of the maxillary second premolars in 440 cases from the patients in Department of Stomatology, The First Affiliated Hospital, Jinan University during March 2011 and December 2017 were collected. The CBCT images were divided into 4 groups according to the patients′ ages: groups ≤20, 21-40, 41-60 and>60 years old, respectively. Changes of morphologies of root canals with aging including such parameters as types of the root canal, incidence of double root canals in single rooted teeth, distance between both root canal orifices of double rooted canals, and morphological change of the cross section of roots.