Objective To explore the cognition impairment of Parkinson's disease (PD) subtypes and the risk factors for cognitive dysfunction.Methods Totally56 patients with PD and 30 control subjects were selected.All patients were assessed by Mini-Mental State Examination (MMSE),the Montreal cognitive assessment scale (MoCA),digital span test scale (DS),block design test scale (BD).The cognition impairment status of PD subtype was compared between the two groups.Risk factors for cognitive impairment in Parkinson's disease were analyzed by the multiple regression analysis.Results The scores (or accuracy) of visuospatial / executive function,memory,repeat,attention,animal naming,understanding,calculation,orientation in PD patients and control group were (29.84±13.78) and (44.63±10.95),(17.64±3.00) and (21.93±2.12),(1.64±0.90) and (2.40±0.62),64.29％ and 86.67％,(2.25±0.77) and (2.70±0.47),(2.66± 0.67) and (2.93±0.25),(3.98±1.17) and (4.93±0.25),(9.59±0.68) and (9.93±0.25) respectively,and there were statistically significant differences in above indictors between two groups (t=-5.080,-7.707,-4.571,-3.374,-2.710,-5.844,-3.367,respectively,x2 =4.86,all P＜0.05).Pearson correlation analysis showed that the cognitive function had a positive correlation with education status,had negative correlations with duration of disease,Unified Parkinson's Disease Rating Scale (UPDRS)-Ⅲ,Hoehn and Yahr (H-Y) staging,Hamilton Anxiety Scale (HAMA),Hamilton Rating Scale for Depression (HRSD) (MMSE:r=0.488,-0.682,-0.478,-0.465,-0.611,-0.538,respectively,all P＜0.05,MoCA:r=0.553,-0.583,-0.396,-0.384,0.499,-0.444,respectively,all P＜0.05),and had no correlation with gender,age,age of onset.Taking the duration of disease,education status,UPDRS-Ⅲ,H-Y grade,HAMA,HRSD as the independent variable,MMSE,MoCA as the dependent variable,multiple linear regression analysis showed that only the duration of disease and education status had effects on cognitive dysfunction in PD (MMSE:β=-0.042,0.196,both P=0.000,MoCA:β=-0.052,0.367,both P＜0.05).Conclusions The key points of cognition impairment in PD patients are the impairments in visuospatial/ executive function,memory,repeat,attention,animal naming,understanding,calculation and orientation.The duration of disease and low level of education are the risk factors for cognitive dysfunction in patients with Parkinson's disease.

Objective To explore the relationship between the concentrations of dust mite allergen and lipopolysaccharide (LPS) in the working place and home for the workers. Methods 30 workers were selected as the experimental group in June, 2005, divided into A and B groups, 15 workers of flour processing in A and 15 workers of leather processing in B respectively, at the same season, 20 teachers from a college were selected as the control group (C group). 360 dust specimen were collected, the concentrations of allergen Der p1, Der f1 were determined by ELISA and the LPS concentration were determined by a kinetic Limulus assay. Results Concentration of allergen Der p1 and Der f1 (median:335.1 and 118.8, 350.0 and 174.1, 31.1and 3.4, 81.5 and 9.6 ?g/g in different sites respectively) in A group was the highest, LPS in B group was the highest (median: 234, 268, 104, 54 EU/mg in different sites respectively). The concentration of allergen in the suspending dust was correlated with those in mattress and floor dust (rs=0.661, P

Objective To explore the clinical applications of the PCT and hs-CRP in inflammatory diseases.Methods The sera of 79 infectious patients were randomly collected.54 samples were bacterial infectious patients,25 samples were nonbacterial infectious patients.The serum levels of PCT and hsCRP in 79 infectious cases were detected by immune fluorescence and Immunoturbidimetry respectively.Results The positive rate of PCT in bacterial infectious patients were much higher than that in nonbacterial infectious patients (x2 =48.337,P ＜ 0.01),and the sensitivity was 92.5％,the specificity was 88.0％ ;The positive rate of hs-CRP in bacterial infectious patients were much higher than that in nonbacterial infectious patients (x2 =22.347,P ＜ 0.01),and the specificity was 88.9％,the specificity was 56.0％.The positive predictive value (88.9％) and negative predictive value (70.0％) of hs-CRP were lower than those of PCT (positive predictive values of 94.3％ and negative predictive value of 84.6％).Conclusion Theserum levels of PCT in inflammatory diseases were significantly increased with an high sensitivity and high specificity.Thus,it may be used as a new marker of inflammatory,which can be helpful in the early evaluation of infectious disease and the observation of curative efficacy.

Objective To study the effect of polysaccharide of Cipangopaludina chinensis on gray human cervix cancer cells(Hela cells) in vitro. Methods Polysaccharide of Cipangopaludina chinensis gray was extracted by water extraction. MTT assay and microscope were used to observe its effect on the growth inhibition of Hela cells at different concentrations of the polysaccharide. Results Polysaccharide of Cipangopaludina chinensis could more significantly inhibit the growth of Hela cells. Conclusion Polysaccharide of Cipangopaludina chinensis could inhibit human cervix cancer cells in vitro

Objective To observe the treatment effect and relapse prevention of cardioversion of atrial fibrillation with Wenxin granules and amiodarone. Methods Sixty patients of chronic atrial fibrillation were divided randomly into treatment of Wenxin granules and amiodarone group (group A) and treatment of simple amiodarone gorup(group B),Thirty cases in each group. All cases were treated with amiodarone by intravenous drip along with oral amiodarone. Thirty cases in group A were treated with Wenxin granules (9 grams,per day) additionally for 4 weeks. Results Patients in group A resumed normal sinus rhythm significantly higher than that in group B. The increasing relapse tendency of atrial fibrillation was higher in group B within 6 months. Conclusion Drug combination with Wenxin granules and amiodarone has better effect than simple amiodarone in the relapse prevention of cardioversion of atrial fibrillation.

Objectives The study aims were to analyze the clinical features and to explore the management of intrapancreatic pseudocysts after acute pancreatitis.Methods A retrospective study was conducted on 151 patients who received surgical treatment from Dec,2008 to Feb,2012 after acute pancreatitis.Based on CT/MRI findings and clinical data,there were 17 patients with intrapancreatic pseudocysts (11.3％).The clinical manifestations,diagnoses and treatments for these 17 patients were retrospectively analyzed,and the outcome after operations were followed.Results For the 17 patients,obstructive jaundice was present in 3 patients,pancreatic portal hypertension (PPH) in 7,pseudoaneurysm in 2.All 17 patients underwent operation.The surgical procedures included internal drainage (n=8),external drainage (n=5),distal pancreatic resection with splenectomy (n=3),and local resection (n=1).All patients recovered after the operations.Conclusions Intrapancreatic pseudocysts after acute pancreatitis had a high incidence of local complication.The diagnosis of these pseudocysts was difficult.The treatment should be performed early for those patients who had developed complications.

Proteins in coagulative and fibrinolytic systems were measured in 50 type 2 diabetic patients and 50 normal controls. Proteins related with fibrinolytic system in the diabetic patients were significantly higher than those in normal controls. The concentration of soluble thrombomodulin (sTM) was negatively correlated with the activity of protein C and positively correlated with plasmin-? 2 -antiplasmin complex in type 2 diabetic patients, suggesting that the increase of sTM is associated with hypercoagulability and enhanced fibrinolysis.

Objective To explore the influence of bacteria lipopolysaccharide on the cytokine production of flour mite allergic rhinitis patients. Methods 8 cases with perennial allergic rhinitis were selected. Then the heparin anticoagulation venous blood was taken from the same patient and was added to fetal calf serum,with ultimate volume 1 ml. The two experimental groups were added 10μg LPS and 1 μg LPS respectively, forming the high LPS dose group and low LPS dose group,while the control group was added nothing. Results The contents of IL-4、IL-5、IL-10、IL-12、IFN-γwere tested by enzyme-linked immunosorbent assay. The findings were that there was significant difference in the productive contents of IL-4、IL-5、IL-10、IL-12 and no significant difference in that of IFN-γ between the high LPS dose group and the low LPS dose group. When compared in two groups,significant difference was found in the productive contents of IL-4 ,IL-10 and IL-12 between the two experimental groups and between the high LPS dose group and the control group, while no obvious difference existed between the low LPS group and control group. However, significant difference was altogether found in the productive content of IL-5 when compared one with another among the three groups,and the result was that low dose of LPS obviously promoted the production of cytokine while high dose of LPS restrained the production of cytokine; no significant difference was found in the production of IFN-γ in all comparisons between the three groups. Conclusion This research, through the experiments in vitro, proved that LPS could effectively adjust the cytokine production of perennial flour mite allergic rhinitis patients and the balance of Th1/Th2 ,whose effect depends on the dose of LPS. This research suggested that the practical future in which special cytokine target treatment will be adopted on perennial flour mite allergic rhinitis patients.

Objective To investigate the effect of livzon weisanlian(bismuth agent 0.22g,bid+tinidazole,0.5g,bid+clarithromycin 0.25g,bid) for 7 days for eradication of H.pylori and infection of patients suffering from chronic gastritis and peptic ulcer in Huannai area.Methods Four pieces of gastric mucosa were taken out by gastroscopy,two pieces of them from gastric antrum 3～5cm near pylori and the others from gastric body.All of them were detected for H.pylori and biopsy and smear.If both the former and the one of the later are all positive the patient was diagnosed with H.pylori and treated with livzon weisanlian.Results The positive of CSG is 81.6%,CAG is 63%,GU is 90.8% and DU is 93.3%.Conclusion The positive rate of patients suffering from chronic gastritis and peptic ulcer in Huainan area is high.The eradicative rate with livzon weisanlian is also high and it shows that livzon weisanlian is feasible to treat chronic gastritis and peptic ulcer.