Objective To investigate the different characteristics of vertebral basilar artery between isolated vertigo and dizziness with ischemic stroke history by magnetic resonance angiography (MRA) information,and find the independent risk factors of isolated vertigo.Methods From January 2015 to January 2016,isolated vertigo patients from our department were enrolled in study group (vertigo group),and dizzy patients with ischemic stroke history in control group (dizziness group).The patient's general information,and the relevant vascula data of the MRA were statistically analyzed to find the risk factors.Results A total of 118 patients with isolated vertigo was enrolled in the vertigo group,and 74 patients with dizziness after ischemic stroke were used as a control group.There were significant statistical differences in mean diameter of the right vertebral artery,rate of stenosis of basilar artery,vertigo history,the left vertebral artery hypoplasia,basilar artery skewing,and basilar artery hypoplasia between two groups (P ＜ 0.05).Multivariate logistic regression analysis showed that vertigo history (P =0.049,OR =3.822,95％ CI =1.004 ～ 14.548),the right vertebral artery hypoplasia (P =0.001,OR =6.083,95％ C1 =2.193 ～ 16.876),the left vertebral artery hypoplasia (P =0.006,OR =5.110,95 ％ CI =1.615 ～ 16.170),and mean diameter of the right vertebral artery (P =O.000,OR =3.143,95％ CI =1.724 ～ 5.730) were independent risk factors for isolated vertigo,and basilar artery skewing (P =0.018,OR =O.436,95％ CI =0.219 ～ O.866),and degree of basilar artery stenosis (P =0.006,0R =0.634,95％ CI =0.459 ～0.877) were the protective factors.Conclusions The right vertebral artery hypoplasia,the left vertebral artery hypoplasia,and mean diameter of the right vertebral artery are independent risk factors for isolated vertigo.Basilar artery skewing and degree of basilar artery stenosis may be the protective factors.

Atherosclerosis (AS) is a multi-factor disease,with complex etiology.It is associated with vascular abnormalities and changing vascular wall composition.AS mainly involve large and medium sized arteries,which may cause multiple organ lesions including heart and brain tissue ischemia and necrosis,myocardial infarction,stroke and other serious diseases.Its pathogenesis has not been finally clarified.This article gives review on pathological changes,etiology and mechanism of AS,as well as its imaging performance.

Objective To explore the clinical manifestation,imaging features and treatment of primary lacri-mal gland epithelial tumor.Methods The clinical data of 25 cases with primary lacrimal gland epithelial tumors were retrospectively studied.Results All of 25 primary lacrimal gland epithelial tumor cases received surgical treatment. Fourteen primary orbital tumors cases were male and 11 cases were female.The mean age was 44 years old (ranged 23 to 65).The mean hospital stay was 12d(ranged 7 to 20).Among 25 primary lacrimal gland epithelial tumor cases, 11 cases were benign tumors which included 4 inflammatory pseudotumor,11 pleomorphic adenoma.Fourteen cases were malignant tumors which included 4 malignant pleomorphic adenoma,6 adenoid cystic carcinoma and 4 adenocar-cinoma.After opeation,visual acuity improved in 9 cases,unchanged 10 cases,decreased 6 cases.The patients were followed up for 16 -48 months(mean 27 months).There were 4 malignant tumors recurrence after operation and received radical operation.While 2 patients were lost and 2 patients died of tumor metastasis,the other 21 patients survived with tumor -free.Conclusion Primary lacrimal gland epithelial tumors have different clinical and imaging appearances.Combination of ultrasound,CT and MRI is important to ascertain the character,range and degree of primary lacrimal gland epithelial tumors.Surgical excision is the main and effective treatment for primary lacrimal gland epithelial tumors,while gamma knife treatment is safe and effective for malignant,unresectable,recurrences tumors.

Objective: To determine the interfering effects of EphB4-targeted short interfering RNA (siRNA) on EphB4 mRNA expression and its effect on the growth of glioma U251 cell line. Method: EphB4-targeted siRNA was designed and synthesized, and then was transfected into U251 cells. The inhibition of EphB4 mRNA expression was detected by RT-PCR. The effect of EphB4-targeted siRNA on cell growth rate was measured by CCK-8 method. Cell apoptosis was tested by flow cytometry (FCM). Wound healing test was used to observe the migration ability of cells. The invasiveness of tumor cells was evaluated by counting the number of cells passing the Transwell membrane. Results: EphB4 mRNA transcription level was decreased by 75.0% after transfection of malignant glioma U251 cells with 100 nmol/L siRNA-EphB4. The inhibition of cell proliferation was in a dose-dependent manner. FCM analysis showed that cells were arrested at sub-G1 phase at different degrees and the migration capacity decreased after transfection with 100 nmol/L siRNA-EphB4 compared with the negative control. The number of cells permeating the matrigel membrane significantly were decreased in the siRNA-EphB4 transfection group compared with the control group. Conclusion: siRNA-EphB4 markedly targetes and knocks down EphB4 gene transcription. Down-regulation of EphB4 affects cell proliferation and induces apoptosis of cells. Transfection of siRNA-EphB4 into U251 cells inhibits the migration and invasion abilities of cells at various degrees. It indicates that silencing EphB4 expression might become a noval approach in the treatment of glioma.

Purpose To study the cytopathologic features of CT-guided percutaneous lung biopsy samples and to evaluate the role of cytopathology in the diagnosis and staging of lung carcinomas, as compared to histopathology. Methods Four-hundred twenty-five specimens were collected by CT-guided percutaneous lung biopsy which were also confirmed by histological diagnosis. Direct smears were performed for each case. Cytological and histological examination was carried out. Results The sensitivity, specificity, false positive rate, false negative rate and accuracy of cytopathology in diagnosing lung carcinomas by CT-guided percutaneous lung biopsy was 86. 6% (264/305), 100% (120/120), 0 (0/120), 13. 4% (41/305), 90. 4% (384/425), respectively. Overall 51. 1%(135/264) of the cases were precisely typed, including 77. 6% (83/107) of adenocarcinoma, 76. 9% (40/52) of squamous cell car-cinoma and 75. 0% (9/12) of small cell carcinoma. Conclusions Cytopathology of CT-guided percutaneous lung biopsy specimens is sensitive and accurate for diagnosing pulmonary carcinomas. In some cases, the lung carcinoma can be precisely typed. Therefore, it is useful for diagnosing and staging lung carcinomas.

Since the concept of "artificial intelligence" was introduced in 1956,it has led to numerous technological innovations in human medicine and completely changed the traditional model of medicine.In this study,we mainly explain the application of artificial intelligence in various fields of medicine from four aspects:machine learning,intelligent robot,image recognition technology,and expert system.In addition,we discuss the existing problems and future trends in these areas.In recent years,through the development of globalization,various research institutions around the world has conducted a number of researches on this subject.Therefore,medical artificial intelligence has attained significant breakthroughs and will demonstrate wide development prospection in the future.

Background Identifying and testing of pain is very necessary for the care and decrease of the suffering of experimental animal in medical experiment.Effective method for testing the pain and distress status of experimental animal with eye disease is still absent in China.Objective This pilot study was to establish an evaluating system for assessing the pain and distress status of bullous keratopathy rabbit model.Methods This study was approved by the Ethic Committee of Beijing University First Hospital.Twelve healthy New Zealand rabbits were selected in this experiment.Bullous keratopathy model was established in the left eyes of 9 rabbits by scraping corneal endothelium as the experimental team,and other 3 rabbits were served as the control team.The cornea lesion was examined by manipulate slit lamp,and the central cornea thickness (CCT) was measured by ultrasound biomicroscopy (UBM).Weight+20 Indexes For Pain and Distress Status Referring Guidelines for Pain and Distress in Laboratory Animals made by International Animal Care and Use Committee (IACUC) were assessed and measured as well.Results Corneal edema and opacity were obvious 1 day after surgery.Corneal bullous appeared from the third day after surgery,and cornea erosion was seen at the location of bullous breakage.The corneal lesions remained until 14 days after surgery.CCT value was (1468±100),(2313±588),(2391±271) and (2362±151) μm,respectively in day 1,3,7 and 14 after the establishment of models,which showed significant increase in comparison with the preoperative CCT (390±6)μm (all P=0.000).However,no significant difference was seen in the CCT between day 3,7 and 14 (P＞0.05).Body weight of the rabbits was (3.29±0.20),(3.20-0.17),(2.77±0.25) and (3.10±0.30)kg respectively in day 1,3,7 and 14 after operation,with significant decrease in comparison with the pre-operative weight (3.52-0.18)kg in the experimental team (P=0.008,0.007,0.003,0.004).The scores for pain and distress status of all rabbits in pre-operation of the experimental team and in the control team were zero,and the score was 7 (7,7),11 (10,12),9 (8,10),9 (9,9)in day 1,3,7 and 14 in the experimental team after surgery,with the highest score in day 3,which was bullous and bullous breakage duration.Seven of twenty indexes,including the reduce of diet and drinking,self-imposed isolation/hiding,grinding teeth,aggression,deceased movement,abnormal posture,vocalization occurred in the model animals after surgery.Conclusions Weight+20 Indexes For Pain and Distress Status is an effective,impersonal and quantitative method for observing and evaluating the pain and stress status in bullous keratopathy rabbit.

OBJECTIVE:To introduce high-value drug insurance access mechanism in Australia,and to provide suggestion for improving orphan drug medical insurance access in China. METHODS:By literature study and policy analysis,access principle, risk-sharing mechanism and restricted use principle of high-value drug insurance access in Australia were summarized and analyzed, so as to evaluate the effects of high-value drug insurance access mechanism. RESULTS & CONCLUSIONS:The inclusion of high-value drug in Australian National Formulary considers not only the cost-effectiveness ratio,but also the drug’s clinical effica-cy,irreplaceability and the social principles;risk-sharing mechanism and restricted use principle achieve a good balance between the accessibility of patient’s medication and the sustainability of health insurance fund;remarkable results are achieved in guarantee-ing patient’s medication and strengthening basic medical insurance. It is recommended to perfect orphan drugs medical insurance ac-cess standard,introduce risk-sharing mechanism and standardize the use of orphan drugs and reimbursement in China.

Objective To investigate the microstructure characteristics in human kidney with the WD cepstrum of ultrasonic backscattering. Methods By using this method, an analysis of the backscattering signals of normal and pathological human kidney in vitro was made and the mean spacing of the scatterers of biological soft tissues was estimated, then compared the means of this new approach with the means of the AR cepstrum. Results The results show that the mean spacings of the scatterers of the two different kidney tissues are noticeably different, which reveals the fact that the WD cepstrum works more effective than the AR cepstrum in showing the features of the microstructure of soft tissues. Conclusion The WD cepstrum is an effective mean to analyze the ultrasonic scattering signals and determine the features of the mean spacing of the scatterers of soft tissues. This method provides more available clinical diagnosis and improves the results of mean spacing of the scatterers of soft tissues.

Air ; analysis ; Air Pollutants, Occupational ; analysis ; Chromatography, Gas ; methods ; Hydrocarbons, Chlorinated ; analysis